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Journals / Turkish Journal of Veterinary and Animal Sciences / 2001 / Cilt: 25 - Sayı: 6

Comparison and Evaluation of the Passive Haemagglutination Test and ELISA in Routine Screening of Contagious Equine Metritis (CEM) in Mares with Endometritis or Infertility

N. Yakut ÖZGÜR
Pages
989–994
Publication Date
2001-06-01
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, the PHA test was recommended for routine screening of CEM because of its easy handling and rapidness.

📌 3 Key Takeaways

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Objective & Hypothesis:One hundred and twenty thoroughbred breeder mares with endometritis or infertility were investigated for the detection of antibodies to Taylorella equigenitalis by the passive haemagglutination (PHA) test and ELISA between 1997 and 1999 in the Marmara region and the two tests were compared.
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Key Findings & Data:Fifty-eight (48.33 %) of 120 sera were found to be positive by the PHA test and 52 (43.33%) by ELISA. 22 of the sera were found to be positive by the PHA test reacted at 1/64 titer, 27 at 1/128, 7 at 1/256 and 2 at 1/512 titer.
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Conclusion & Impact:In conclusion, the PHA test was recommended for routine screening of CEM because of its easy handling and rapidness.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Taylorella equigenitalis · Contagious equine metritis · passive haemagglutination test

Abstract

One hundred and twenty thoroughbred breeder mares with endometritis or infertility were investigated for the detection of antibodies to Taylorella equigenitalis by the passive haemagglutination (PHA) test and ELISA between 1997 and 1999 in the Marmara region and the two tests were compared. Fifty-eight (48.33 %) of 120 sera were found to be positive by the PHA test and 52 (43.33%) by ELISA. 22 of the sera were found to be positive by the PHA test reacted at 1/64 titer, 27 at 1/128, 7 at 1/256 and 2 at 1/512 titer. Sera from 44 mares were positive in both tests. Amongst these, 14 sera were positive in the PHA test and 8 sera were positive in ELISA. The difference between the PHA test and ELISA positivity was 8% and this was not statistically significant. In conclusion, the PHA test was recommended for routine screening of CEM because of its easy handling and rapidness.

Özet

One hundred and twenty thoroughbred breeder mares with endometritis or infertility were investigated for the detection of antibodies to Taylorella equigenitalis by the passive haemagglutination (PHA) test and ELISA between 1997 and 1999 in the Marmara region and the two tests were compared. Fifty-eight (48.33 %) of 120 sera were found to be positive by the PHA test and 52 (43.33%) by ELISA. 22 of the sera were found to be positive by the PHA test reacted at 1/64 titer, 27 at 1/128, 7 at 1/256 and 2 at 1/512 titer. Sera from 44 mares were positive in both tests. Amongst these, 14 sera were positive in the PHA test and 8 sera were positive in ELISA. The difference between the PHA test and ELISA positivity was 8% and this was not statistically significant. In conclusion, the PHA test was recommended for routine screening of CEM because of its easy handling and rapidness.

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Cite This Article

ÖZGÜR, N. Y. (2001). Comparison and Evaluation of the Passive Haemagglutination Test and ELISA in Routine Screening of Contagious Equine Metritis (CEM) in Mares with Endometritis or Infertility. Turkish Journal of Veterinary and Animal Sciences, Cilt: 25 - Sayı: 6, 989–994. https://ajindex.com/articles/227876

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