Human papillomavirus (HPV) ve Gebelik; prevalans ve tanı yöntemleri

Objective & Hypothesis:OBJECTIVE: To investigate the prevalence of Human Papillomavirus (HPV) and type detection of Human Papillomavirus as the most important causative agent of cervical cancer by PCR in pregnant women applying to Obstetrics and Gynecology outpatient clinic in Gazi Hospital.

📊

Key Findings & Data:MATERIAL AND METHOD: Two hundred asymptomatic pregnant women applying to Obstetrics and 102 asymptomatic women applying to Gynecology outpatient clinic were included.

💡

Conclusion & Impact:However, asymptomatic but HPV positive patients were directed to clinicians for follow-up and treatment.

🔬Design:Research Article

📍Scope:Comprehensive Sample

⚙️Variables:Domain Parameters

Abstract

OBJECTIVE: To investigate the prevalence of Human Papillomavirus (HPV) and type detection of Human Papillomavirus as the most important causative agent of cervical cancer by PCR in pregnant women applying to Obstetrics and Gynecology outpatient clinic in Gazi Hospital. MATERIAL AND METHOD: Two hundred asymptomatic pregnant women applying to Obstetrics and 102 asymptomatic women applying to Gynecology outpatient clinic were included. HPV DNA was extracted from cervical smear samples by using phenol chloroform isoamyl alcohol and amplified by MY09/11 primers. Specific primers were used for type 16/18 detection. RESULTS: Five (2.5%) of 200 pregnant women were HPV DNA (+), two (1%) of which were type 16,two (1%) of which were type 18 and one(0.5%) of which was type 11.Two(2%) of 102 women in contol group were positive, both of which were type 16. CONCLUSION: HPV prevalence in pregnant women is lower than indicated in the literature. The probable reason may be common monogamy in Turkey and the non-smoking, young patient profile with high social and economic level in our study group. However, asymptomatic but HPV positive patients were directed to clinicians for follow-up and treatment.

Özet

AMAÇ: Gazi Üniversitesi Tıp Fakültesi Kadın Hastalıkları ve Doğum polikliniğine başvuran gebelerde serviks kanseri etiyolojisinde çok önemli rolü olan Human papillomavirus (HPV)’un Polimeraz Zincir Reaksiyonu (PCR) yöntemiyle tespiti ve tip tayini amaçlanmıştır. GEREÇ ve YÖNTEM: Rutin kontrol için Gazi Üniversitesi Tıp Fakültesi Kadın Hastalıkları ve Doğum polikliniğine başvuran asemptomatik 200 gebe ve 102 kontrol hastasından alınan sürüntü örneklerinden fenol kloroform izoamilalkol yöntemiyle DNA izolasyonu yapılmış ve MY 09/11 primerleri ile HPV DNA amplifikasyonu yapılmıştır. Tip 16 ve 18 tayini için özgül primerler kullanılmıştır. BULGULAR: Çalışmaya alınan 200 gebenin beşinde (%2.5) HPV DNA pozitif bulunmuştur. Pozitif örneklerin ikisi (%1) tip 16, ikisi (%1) tip 18, biri (%0.5) tip 11 olarak tespit edilmiştir. Yüziki kişilik kontrol grubunda iki (%2) hastada HPV DNA pozitif bulunmuştur. Pozitif örneklerin her ikisi de tip 16 olarak tespit edilmiştir SONUÇ: Sonuçlarımız literatürdeki oranlara göre düşüktür. Bunun nedeni Türkiye’de tek eşliliğin yaygın olması ve hasta grubumuzun yüksek sosyoekonomik düzeye sahip sigara içmeyen kadınlardan oluşması olabilir. Bununla birlikte, asemptomatik olduğu halde virus tesbit edilen hastalar tedavi ve takip açısından yönlendirilmişlerdir.

🔖

Cite This Article

BOZDAYI, G., DİNÇ, B., ROTA, S., BİRİ, A., BAYRAMOĞLU, Ö., GÜRSOY, R. (2009). Human papillomavirus (HPV) ve Gebelik; prevalans ve tanı yöntemleri. GORM:Gynecology Obstetrics & Reproductive Medicine, Cilt: 15 - Sayı: 1, 8–11. https://ajindex.com/articles/227558

📄 Full Text PDF Viewer

Open in New Tab ↗

↗New Tab

📄

PDF görüntüleyici hazırlanıyor...

References (15)

Hagensee M, Slavinsky J, Goffga C, Suros J, Kissinger P, Martin D. Seroprevalence of Human Papillomavirus Type 16 in Pregnant Women. Obstetrics and Gynecology 1999;94:653-658
Sampson H, Gravett B: Other infectious conditions in pregnancy, In James DK, Steer PJ, Weiner CP, Gonik B. (Ed), High Risk Pregnancy. 1999. Second Edition
Eppel W, Worda C, Frigo P, Ulm M, Kucera E, Czerwenka K. Human Papillomavirus in the cervix and placenta. Obstetrics and Gynecology 2000;96:337-341
Mayeaux EJ. Reducing the economic burden of HPV-related diseases. J Am Osteopath Assoc 2008;108 (4 Suppl 2):52-7
Armbruster-Moroes E, Ioshimoto LM, Leao E, Zugaib M. Presence of Human Papillomavirus DNA in amniotic fluid of pregnant women with cervical lesions. Gynecol Oncol 1994;54:152-8
Chan PK, Chang AR, Tam WH, Cheung JLK, Cheng AF. Prevalence and Genotype Distribution of Cervical Human Papillomavirus Infection: Comparison Between Pregnant Women and Non-Pregnant Controls. Journal of Medical Virology 2002;67:583-588
Ruffin MT, Bailey JM, Roulston D et al. Human papillomavirus in amniotic fluid. BMJ Pregnancy Childbirth 2006; 6: 28
Beumer KR,Tyring S. Human Papillomavirus and human disease. Am J Med. 1997;102:9-15
Kemp EA, Hakenewerth AM, Laurent SL, Gravitt PE, Stoerker J. Human Papillomavirus prevalence in pregnancy. Obstet Gynecol 1992;79:649-56
Smith EM, Ritchie JM, Yankowitz J, Wang D, Turek LP, Haugen TH. HPV prevalence and concordance in the cervix and oral cavity of pregnant women. Infect Dis Obstet Gynecol 2004; 12: 45-56
Gajewska M, Wielgos M, Kaminski P et al. The occurance of genital types of Human papillomavirus in normal pregnancy and in pregnant renal transplant recipients. Neuro Endocrinol Lett 2006; 27(4): 529-34
Takakuwa K, Mitsui T, Iwashita M et al. Studies on the prevalence of Human papillomavirus in pregnant women in Japan. J Perinat Med 2006; 34(1): 77-9
Fife KH, Katz BP, Roush J, Handy VD, Brown DR, Hansell R. Cancer associated Human Papillomavirus types are selectively increased in the cervix of women in the first trimester of pregnancy. Am J Obstet Gynecol. 1996; 174:1487-93
Fife KH, Katz BP, Brizendine EJ, Brown DR. Cervical Human Papillomavirus deoxyribonucleic acid persists throughout pregnancy and decreases in the postpartum period. Am J Obstet Gynecol 1999; 180:1110-14
Morrison EA, Gammon MD, Goldberg GL, Vermund SH, Burk RD. Pregnancy and cervical infection with Human Papillomaviruses. Int