Journals / GORM:Gynecology Obstetrics & Reproductive Medicine / 2009 / Cilt: 15 - Sayı: 2

Human Papilloma Virüsün Rutin Jinekolojik Kontrole Gelen Kadınlarda Sitolojik ve Klinik Olarak Değerlendirilmesi

Cytologic and Clinical Evaluation of Human Papillomavirus in Women Underwent Routine Gynecologic Examination

Pages
200915294–200915299
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

CONCLUSION: Pap smear examination is a value of detection of cellular changes belonging Human Papillomavirus and if in this examination it is need to, these patients refer to Human Papillomavirus DNA screening it would be more cost effective.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:OBJECTIVE: To determine the frequency of Human Papillomavirus infection in routine gynecologic examination and to detect whether there is a relationship between the presence of Human Papilloma Virus and cytologic and gynecological findings.
📊
Key Findings & Data:Conventional Pap smears of 400 patients were stained by Papanicolaou technique and examined cytologically.
💡
Conclusion & Impact:CONCLUSION: Pap smear examination is a value of detection of cellular changes belonging Human Papillomavirus and if in this examination it is need to, these patients refer to Human Papillomavirus DNA screening it would be more cost effective.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

OBJECTIVE: To determine the frequency of Human Papillomavirus infection in routine gynecologic examination and to detect whether there is a relationship between the presence of Human Papilloma Virus and cytologic and gynecological findings. STUDY DESIGN: Four hundred women applying to Gynecology outpatient clinic for routine gynecologic examination were included. Conventional Pap smears of 400 patients were stained by Papanicolaou technique and examined cytologically. HPV DNA was detected by in-house PCR . Also HPV positive samples were evaluated for HPV 16 and 18 by same method. RESULTS: Eleven (2.75%) of 400 women were HPV DNA (+). Two of which were type 16 and 18 and one of which was type 18. Nuclear membrane irregularity, hyperchromatism, koilocytotic cell, vacuolisation and post partum period corralated with HPV infection (p <0.05). CONCLUSION: Pap smear examination is a value of detection of cellular changes belonging Human Papillomavirus and if in this examination it is need to, these patients refer to Human Papillomavirus DNA screening it would be more cost effective.

Özet

AMAÇ: Rutin jinekolojik incelemede Human Papilloma Virus (HPV) sıklığını belirlemek ve bu virüs ile hücresel değişiklikler, sitolojik ve jinekolojik bulgular arasında ilişki olup olmadığını değerlendirmek. GEREÇ VE YÖNTEM: Rutin jinekolojik muayeneleri için Ji ne - koloji kliniğine başvuran dörtyüz hasta çalışma kapsamına alınmıştır. Bu hastalara ait konvansiyonel serviko-vajinal yaymalar Papanicolaou yöntemine göre boyanmış ve sitolojik olarak incelenmiştir. HPV DNA Polimeraz Zincir Reaksiyonu (PCR) ile değerlendirilmiştir. HPV pozitif örnekler ise yine aynı yöntemle HPV tip 16 ve 18 açısından incelenmiştir. BULGULAR: HPV DNA 400 hastanın 11 (%2.75)'inde saptan - mış tır. HPV pozitif hastalar HPV 16 ve 18 açısından PCR ile değerlendirildiğinde ise 2 hastada HPV 16 ve 18 birlikte bulunurken 1 hastada sadece HPV 18 saptanmıştır. HPV ile çekirdek zar düzensizliği, hiperkromatizm, koilositotik hücre, vakuolizasyon ve post partum dönemde bulunma arasında anlamlı ilişki saptanmıştır (p<0.05). SONUÇLAR: Pap simir incelemesinin HPV'a ait hücresel değişikliklerin tesbiti için değer taşıdığı ve eğer simir taramasında şüphelenilirse hastanın HPV DNA taramasına yönlendirilmesinin maliyet açısından da yararlı olacağı düşüncesindeyiz.

🔖

Cite This Article

ÖZ, Z. S., DEMİREZEN, S., KUZEY, G. M., BEKSAÇ, M. S., USTAÇELEBİ, Ş. (2009). Human Papilloma Virüsün Rutin Jinekolojik Kontrole Gelen Kadınlarda Sitolojik ve Klinik Olarak Değerlendirilmesi. GORM:Gynecology Obstetrics & Reproductive Medicine, Cilt: 15 - Sayı: 2, 200915294–200915299. https://ajindex.com/articles/227050

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (1)

  1. Jenkins D. Diagnosing Human Papillomaviruses: Recent advances. Curr Opin Infect Dis 2001, 14:53-62 2. Burd E.Human Papillomavirus and Cervical Cancer. Clin Microbiol Rev 2003,Jan:1-17 3. Ergunay K, Misirlioglu M, Firat P et al. Detection and typing of human papilloma virus by Polymerase chain reaction and hybridization assay in cervical samples with cytological abnormalities. Mikrobiyol Bul 2008;42: 273-282 4. Hoory T, Monie A, Grawitt P, Wu TC. Molecular epidemiology of human papillomavirus. J Formos Med Assoc 2008; 107:198-217 5. Howley PM: Papillomavirinae: The viruses and Their Replication. In: Fields BN, Knipe DM, Howley PM (Eds): Fundamental Virology. 3th ed. Philadelphia, JB Lippincott Company, 1996;947-978 6. Gammoh N, Grm HS, Massimi P, Banks L: Regulation of Human papillomavirus type 16 E7 activity through direct protein interaction with the E2 transcriptional activator. J Virol 2006; 1787-1797 7. Flores E, Allen-Hoffmann L, Lee D, Lambert P. The Human Papillomavirus type 16 E7 oncogene is required for the productive stage of the viral life cycle. J Virol 2000; 74:6622-6631 8. Stanley M. Human Papillomavirus and cervical carcinogenesis. Best Pract Res Clin Obstet Gynecol 2001;15: 663-676 9. Andersson S, Mints M, Sallström J, Wilander E. The relative distribution of oncogenic types of human papillomavirus in benign, pre malignant and malignant cervical biopsies A study with human papillomavirus deoxyribonucleic acid sequence analysis. Cancer Detect Prev 2005; 29: 37-41 10. Guner H, Tiftik Z, Erdem M, Edalı N. The incidence of Human Papillomavirus infection in Turkish women. Anatolian J Gynecol Obst 1992; 2:178-181 11. Ozcelik B, Serin IS, Gokahmetoglu S, Basbug M, Erez R. Human papillomavirus frequency of women at low risk of developing cervical cancer: a preliminary study from a Turkish university hospital. Eur J Gynaecol Oncol 2003;24:157-159 12. Ozturk S, Kaleli I, Kaleli B, Bir F. Investigation of human papillomavirus DNA in cervical specimens by hybrid capture assay. Microbiyol Bul 2004; 38;223-232 13. Winer RL, Lee SK, Hughes JP, Adam DE, Kiviat NB and Koutsky LA. Genital Human Papillomavirus Infection: Incidence and Risk Factors in a Cohort of Female University Students. Am J Epidemiol 2003;157:218-226 14. Goodman MT, Shvetsov YB, McDuffie K et al. Prevalence, Acquisition, and Clearance of Cervical Human Papillomavirus Infection among Women with Normal Cytology: Hawaii Human Papillomavirus Cohort Study. Cancer Res 2008;68(21):8813-24] 15