Journals / Flora İnfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi / 1997 / Cilt: 2 - Sayı: 3

Bacitracin and PYR tests for the diagnosis of group A beta haemolytic streptococci in throat cultures

Boğaz kültürlerinde üreyen A grubu beta hemolitik streptokokların tanısında basitrasin ve PYR testleri

Pages
176–182
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In throat cultures, unless a special request is recommended, only group A streptococci are investigated.

📌 3 Key Takeaways

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Objective & Hypothesis:In throat cultures, unless a special request is recommended, only group A streptococci are investigated.
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Key Findings & Data:In accordance with that possibility, performing the routinely used bacitracin and a more rapid PYR test on human blood and sheep blood containing culture media, specificity and sensitivity of those tests were investigated, The highest sen¬sitivity (96.2%) and specificity (97.7%) was found as belonging to PYR test applied on sheep blood containing culture media.
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Conclusion & Impact:On the other hand, in accordence with the cost, using sheep blood containing culture media and performing bacitracin test, was found as the most suitable procedure.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In throat cultures, unless a special request is recommended, only group A streptococci are investigated. In this study, our aim was to determine the routine culture and test procedures of a laboratory for throat cultures with minimum cost and reliable results. Although not recommended, in our country in most of the laboratories, for throat cultures human blood containing culture media are used. In accordance with that possibility, performing the routinely used bacitracin and a more rapid PYR test on human blood and sheep blood containing culture media, specificity and sensitivity of those tests were investigated, The highest sen¬sitivity (96.2%) and specificity (97.7%) was found as belonging to PYR test applied on sheep blood containing culture media. On the other hand, in accordence with the cost, using sheep blood containing culture media and performing bacitracin test, was found as the most suitable procedure.

Özet

Özel istek belirtilmedikçe tüm boğaz kültürlerinde yalnızca A grubu beta hemolitik streptokoklar aran¬maktadır. Çalışmamızda bir kültür laboratuvarında boğaz kültürü sonuçlarının güvenilir ve minimum maliyet¬le verilebilmesi için boğaz kültürlerinin rutin ekim ve test koşullarını belirlemeyi amaçladık. Boğaz kültürleri¬nin ekimi için önerilmediği halde ülkemizde pek çok laboratuvarda kullanılan insan kanlı besiyerlerinde ve kul¬lanılması önerilen koyun kanlı besiyerlerinde, rutinde uygulanan basitrasin ve daha hızlı sonuç veren PYR test¬leri uygulanarak bu testlerin özgüllük ve duyarlılıkları araştırıldı. Koyun kanlı besiyeri kullanıldığında ve PYR testi uygulandığında hem duyarlılık (%97.7) hem de özgüllüğün (%96.2) yüksek olduğu bulundu. Maliyet anali¬zi gözönüne alındığında ise en uygun yöntemin koyun kanlı besiyeri kullanılarak basitrasin testi uygulanması olduğu saptandı.

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Cite This Article

ERDİNÇ, F. Ş., ÇEVİK, M. A., KINIKLI, S., ACAR, N., ORAL, B. (1997). Bacitracin and PYR tests for the diagnosis of group A beta haemolytic streptococci in throat cultures. Flora İnfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi, Cilt: 2 - Sayı: 3, 176–182. https://ajindex.com/articles/224415