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Journals / Flora İnfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi / 1999 / Cilt: 4 - Sayı: 4

Determination of TT virus (TTV) prevalence in blood donors in Istanbul

İstanbul' da kan donörlerinde TT virüsü (TTV) prevalansının araştırılması

Semra TUNÇBİLEK
Diler COŞKUN
Fuat ÇETİNKAYA
Nedret HIZEL
Pelin TAHTAKILIÇ
Pages
273–277
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Besides epidemiological studies, it's also necessary to determine the relation of TT virus with hepatic and extra hepatic diseases.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, TT virus-DNA was detected in the sera of 200 blood donors in İstanbul, 68 (34%) from Haydarpaşa Numune Hospital and 132 (66%) from Red Crescent Zeynep Kamil blood centers, by semi-nested polymerase chain reaction method.
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Key Findings & Data:A total of 20 pools, each containing 10 serum samples were prepared.
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Conclusion & Impact:Besides epidemiological studies, it's also necessary to determine the relation of TT virus with hepatic and extra hepatic diseases.
🔬Design:Lab & Field Experiment
📍Scope:Comprehensive Sample
⚙️Variables:Karaciğer hastalıkları · Kan vericileri · Polimeraz zincir reaksiyonu

Abstract

In this study, TT virus-DNA was detected in the sera of 200 blood donors in İstanbul, 68 (34%) from Haydarpaşa Numune Hospital and 132 (66%) from Red Crescent Zeynep Kamil blood centers, by semi-nested polymerase chain reaction method. A total of 20 pools, each containing 10 serum samples were prepared. Eight pools were positive by initial screening, among which 9 individual samples were found to contain TTV-DNA by further analysis. This is the first study which determines the prevalence of TT virus in our country. Besides epidemiological studies, it's also necessary to determine the relation of TT virus with hepatic and extra hepatic diseases.

Özet

Bu çalışmada 68 (%34)'i Haydarpaşa Numune Hastanesi Kan Merkezi'nden, 132 (%66)'si Kızılay Zeynep Kamil Kan Merkezi'nden olmak üzere, İstanbul'da toplam 200 kan donöründe "semi-nested" polimeraz zincir reaksiyonu yöntemi ile TT virüsü-DNA'sı araştırılmıştır. Onar serum örneği içeren 20 havuz hazırlanmış, bunların sekizinde pozitif sonuç alınmış ve bu havuzların içeriğinde yer alan serumlar tek tek açıldığında, toplam dokuz (%4.5) serumun TTV-DNA'sı içerdiği belirlenmiştir. Bu, ülkemizde kan donörlerinde TT virüsü prevalansının araştırıldığı ilk çalışmadır. Bir yandan epidemiyolojik çalışmalar sürdürülürken, bir yandan da TT virüsünün hepatik ve ekstrahepatik hastalıklarla ilişkisinin belirlenmesi gerekmektedir.

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Cite This Article

TUNÇBİLEK, S., COŞKUN, D., ÇETİNKAYA, F., HIZEL, N., TAHTAKILIÇ, P. (1999). Determination of TT virus (TTV) prevalence in blood donors in Istanbul. Flora İnfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi, Cilt: 4 - Sayı: 4, 273–277. https://ajindex.com/articles/224377

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