Journals / Turkish Journal of Veterinary and Animal Sciences / 2002 / Cilt: 26 - Sayı: 5

Application of Western Blotting for the Immunodiagnosis of Fasciola hepatica in Cattle Using Excretory/Secretory Antigens

Pages
1061–1065
Publication Date
2002-05-01
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The bands obtained from the sera of positive and negative groups were compared and the specific protein bands for F. hepatica infection were 36, 29 and 17 kDa.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, protein bands of excretory/secretory antigens of Fasciola hepatica were determined using SDS-PAGE and Western blotting.
📊
Key Findings & Data:After examining the organs and faecal samples of these cattle for Fasciola hepatica and other helminths, serum samples were divided into two groups as positive (10 cattle) and negative (5 cattle) for F. hepatica.
💡
Conclusion & Impact:The bands obtained from the sera of positive and negative groups were compared and the specific protein bands for F. hepatica infection were 36, 29 and 17 kDa.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Cattle · Fasciola hepatica · SDS-PAGE

Abstract

In this study, protein bands of excretory/secretory antigens of Fasciola hepatica were determined using SDS-PAGE and Western blotting. Blood and faecal samples were obtained from cattle brought to Kazan slaughterhouse. After examining the organs and faecal samples of these cattle for Fasciola hepatica and other helminths, serum samples were divided into two groups as positive (10 cattle) and negative (5 cattle) for F. hepatica. The sera of these two groups were tested using Western blotting. The bands obtained from the sera of positive and negative groups were compared and the specific protein bands for F. hepatica infection were 36, 29 and 17 kDa.

Özet

In this study, protein bands of excretory/secretory antigens of Fasciola hepatica were determined using SDS-PAGE and Western blotting. Blood and faecal samples were obtained from cattle brought to Kazan slaughterhouse. After examining the organs and faecal samples of these cattle for Fasciola hepatica and other helminths, serum samples were divided into two groups as positive (10 cattle) and negative (5 cattle) for F. hepatica. The sera of these two groups were tested using Western blotting. The bands obtained from the sera of positive and negative groups were compared and the specific protein bands for F. hepatica infection were 36, 29 and 17 kDa.

🔖

Cite This Article

SARIMEHMETOĞLU, H. O. (2002). Application of Western Blotting for the Immunodiagnosis of Fasciola hepatica in Cattle Using Excretory/Secretory Antigens. Turkish Journal of Veterinary and Animal Sciences, Cilt: 26 - Sayı: 5, 1061–1065. https://ajindex.com/articles/220643

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...