Journals / Turkish Journal of Veterinary and Animal Sciences / 2000 / Cilt: 24 - Sayı: 5

Analysis of Fluids of Hydatid Cysts from Sheep by SDS-PAGE, and Determination of Specific Antigens in Protein Structure by Western Blotting

Pages
493–500
Publication Date
2000-05-01
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

After putting these research results into practice, there will be no need to import expensive ready-made kits for the diagnosis of hydatid disease in humans.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Protein bands of hydatid fluids of sheep liver were determined by SDS-PAGE and Western blotting, and this was followed by the determination of specific bands of hydatid cysts for sheep and humans.
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Key Findings & Data:For diagnostic purposes, 30 positive and 20 negative sheep sera, 1 commercially obtained non-infected sheep serum, and 10 posi- tive and 10 negative human sera were used in this experiment.
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Conclusion & Impact:After putting these research results into practice, there will be no need to import expensive ready-made kits for the diagnosis of hydatid disease in humans.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Hydatid cyst · SDS-PAGE · Western blotting

Abstract

Protein bands of hydatid fluids of sheep liver were determined by SDS-PAGE and Western blotting, and this was followed by the determination of specific bands of hydatid cysts for sheep and humans. For diagnostic purposes, 30 positive and 20 negative sheep sera, 1 commercially obtained non-infected sheep serum, and 10 posi- tive and 10 negative human sera were used in this experiment. According to our results, the specific protein band for hydatid dis- ease in sheep was determined as 116 kDa, while the specific protein bands for hydatid disease in humans were determined as 68 and 8 kDa. We determined that the diagnosis of hydatid disease in sheep and humans is possible with the use of the purified specific proteins obtained in this study. After putting these research results into practice, there will be no need to import expensive ready-made kits for the diagnosis of hydatid disease in humans.

Özet

Protein bands of hydatid fluids of sheep liver were determined by SDS-PAGE and Western blotting, and this was followed by the determination of specific bands of hydatid cysts for sheep and humans. For diagnostic purposes, 30 positive and 20 negative sheep sera, 1 commercially obtained non-infected sheep serum, and 10 posi- tive and 10 negative human sera were used in this experiment. According to our results, the specific protein band for hydatid dis- ease in sheep was determined as 116 kDa, while the specific protein bands for hydatid disease in humans were determined as 68 and 8 kDa. We determined that the diagnosis of hydatid disease in sheep and humans is possible with the use of the purified specific proteins obtained in this study. After putting these research results into practice, there will be no need to import expensive ready-made kits for the diagnosis of hydatid disease in humans.

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Cite This Article

BURGU, A., DOĞANAY, A., GÖNENÇ, B., SARIMEHMETOĞLU, H. O., KALINBACAK, F. (2000). Analysis of Fluids of Hydatid Cysts from Sheep by SDS-PAGE, and Determination of Specific Antigens in Protein Structure by Western Blotting. Turkish Journal of Veterinary and Animal Sciences, Cilt: 24 - Sayı: 5, 493–500. https://ajindex.com/articles/398063

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