Journals / Flora İnfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi / 2000 / Cilt: 5 - Sayı: 2

The evaluation of the Staphylococcus aureus strains by using arbitrarily primed-polymerase chain reaction and plasmid profile analysis techniques

Metisilin dirençli Staphylococcus aureus'ların arbitrarily primed PCR ve plazmid profil analizi ile değerlendirilmesi

Pages
104–109
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It was found that as nosocomial pathogens group I and II (77%) are frequently encountered for AP-PCR, while group A for PPA (73%) dominated.

📌 3 Key Takeaways

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Objective & Hypothesis:A total of 30 methicillin resistant Staphylococcus aureus (MRSA) strains were isolated as nosocomial pathogens from patients hospitalized in General Surgery, Orthopedics and Reconstructive Surgery Departments from June 1997 through December 1997.
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Key Findings & Data:These strains were evaluated by moleculer typing methods using arbitrarily primed-polymerase chain reaction (AP-PCR) and plasmid profile analysis (PPA).
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Conclusion & Impact:It was found that as nosocomial pathogens group I and II (77%) are frequently encountered for AP-PCR, while group A for PPA (73%) dominated.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Metisilin direnci · Staphylococcus aureus · Rastgele genişletilmiş polimorfik DNA tekniği

Abstract

A total of 30 methicillin resistant Staphylococcus aureus (MRSA) strains were isolated as nosocomial pathogens from patients hospitalized in General Surgery, Orthopedics and Reconstructive Surgery Departments from June 1997 through December 1997. These strains were evaluated by moleculer typing methods using arbitrarily primed-polymerase chain reaction (AP-PCR) and plasmid profile analysis (PPA). By using AP-PCR method, these isolates on the basis of their band patterns were divided into seven groups (I through VII) in which each group had reciprocal number of isolates by thirteen, ten, two, two, one, öneand one, respectively. By PPA, these isolates were grouped into three divisions as group A, B and C and each was represented by twentytwo, three and five isolates respectively. It was found that as nosocomial pathogens group I and II (77%) are frequently encountered for AP-PCR, while group A for PPA (73%) dominated.

Özet

Bu çalışmada; hastanemizin Genel Cerrahi, Ortopedi ve Travmatoloji ile Plastik ve Rekonstrüktif Cerrahi kliniklerinde yatarak tedavi gören 30 hastadan Haziran-Aralık 1997 tarihleri arasında hastane infeksiyonu etkeni olarak izole edilen ve tanımlanan 30 metisiline dirençli Staphylococcus aureus (MRSA) izolatı "arbitrarily primed-polymerase chain reaction (AP-PCR)" ve plazmid profil analizi (PPA) yöntemleriyle moleküler olarak tiplendirilmiştir. Hastalardan infeksiyon etkeni olarak izole edilen 30 MRSA izolatı AP-PCR yöntemi ile bant paternlerine göre yedi grupta (1. grupta: 13,11. grupta: 10, 111. grupta: 2, IV. grupta: 2, V. grupta: I, VI. grupta: 1 ve VII. grupta: I), PPA yöntemi ile ise plazmid profiline göre üç grupta (A grubunda: 22, B grubunda: 3, C grubunda: 5) toplanmıştır. Çalışma sonucunda ilgili kliniklerdeki hastalardan elde edilen izolatların, özellikle 1. ve II. grup ile A grubunda (sırasıyla AP-PCR %77, PPA %73) yer aldığı belirlenmiş ve bu izolatların hastanemizde hastane infeksiyonlarına en sık neden olan kökenler olduğu saptanmıştır.

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Cite This Article

KISA, Ö., BAŞUSTAOĞLU, A., ÖZYURT, M., HAZNEDAROĞLU, T. (2000). The evaluation of the Staphylococcus aureus strains by using arbitrarily primed-polymerase chain reaction and plasmid profile analysis techniques. Flora İnfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi, Cilt: 5 - Sayı: 2, 104–109. https://ajindex.com/articles/219551

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