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Journals / Etlik Veteriner Mikrobiyoloji Dergisi / 2020 / Cilt: 31 - Sayı: 2

An evaluation of the effects of medium pH on the viability of the HepG2 cell line

HepG2 hücre hattının canlılığı üzerine medium pH'sının etkisinin incelenmesi

Neşe GÜVENALP
Dilek GÜVENÇ
Pages
107–114
Publication Date
2020-12-31
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

At pH 6.8 and 6.6, HepG2 cell viability was not significantly different from the control group (pH 7.2) (p>0.05) but there was a significant decrease at pH 6.4, 6.2 and 6.0 (p 0.05), and decreased at pH 7.6 and 7.8 (significant at pH 7.8; p

📌 3 Key Takeaways

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Objective & Hypothesis:Cancer cells are characterized by increased glucose uptake and the production of lactate, which leads to acidification of the tumor microenvironment.
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Key Findings & Data:In this study, we investigated the effects of medium pH manipulation on the proliferation and viability of human hepatocellular carcinoma (HepG2) cells in vitro.
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Conclusion & Impact:At pH 6.8 and 6.6, HepG2 cell viability was not significantly different from the control group (pH 7.2) (p>0.05) but there was a significant decrease at pH 6.4, 6.2 and 6.0 (p 0.05), and decreased at pH 7.6 and 7.8 (significant at pH 7.8; p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Alkali ortam · HepG2 · mikro-çevre

Abstract

Cancer cells are characterized by increased glucose uptake and the production of lactate, which leads to acidification of the tumor microenvironment. This acidification facilitates the development of invasiveness and metastasis. In this study, we investigated the effects of medium pH manipulation on the proliferation and viability of human hepatocellular carcinoma (HepG2) cells in vitro. HepG2 cells were grown in media with pH ranging from 6.0 to 8.5 for 24 h. The cells were then subjected to WST-1 and trypan blue exclusion assays to evaluate viability and cell proliferation, respectively. At pH 6.8 and 6.6, HepG2 cell viability was not significantly different from the control group (pH 7.2) (p>0.05) but there was a significant decrease at pH 6.4, 6.2 and 6.0 (p 0.05), and decreased at pH 7.6 and 7.8 (significant at pH 7.8; p

Özet

Kanser hücreleri, tümör mikroçevresinin asitleşmesine neden olan artmış glikoz alımı ve laktat üretimi ile karakterizedir. Asidik mikro-çevre kanser hücrelerinin invazyon ve metastaz gelişimini kolaylaştırır. Bu çalışmada medium pH değişiminin, in vitro insan hepatoselüler karsinom (HepG2) hücrelerinin proliferasyon ve canlılığına etkisinin değerlendirilmesi amaçlanmıştır. HepG2 hücreleri pH değeri 6.0 ila 8.5 arasında değişen medium ortamlarında 24 saat boyunca inkübe edildi. Daha sonra hücre canlılığı ve proliferasyon değerlendirmeleri için sırasıyla WST-1 ve tripan mavisi hücre canlılık testleri uygulandı. HepG2 hücre canlılığında, medium pH’sı 6.6 ve 6.8 olan gruplar ile kontrol grubu (pH 7.2) karşılaştırıldığında önemli bir farkın bulunmadığı (p> 0.05), ancak pH 6.4, 6.2, 6.0'da önemli oranda azalmanın olduğu saptandı (p 0.05), pH 7.6 ve 7.8'de azalmanın bulunduğu ve bu azalmanın pH 7.8’de önemli olduğu saptandı (p

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Cite This Article

GÜVENALP, N., GÜVENÇ, D. (2020). An evaluation of the effects of medium pH on the viability of the HepG2 cell line. Etlik Veteriner Mikrobiyoloji Dergisi, Cilt: 31 - Sayı: 2, 107–114. https://ajindex.com/articles/218134

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References (8)

  1. Alfarouk KO, Stock CM, Taylor S, Walsh M, Muddathir AK, Verduzco D, Bashir AH, Mohammed OY, Elhassan GO, Harguindey S, Reshkin SJ, Ibrahim ME, Rauchet C. (2015). Resistance to cancer chemotherapy: failure in drug response from ADME to P-gp. Cancer Cell Int. 15,71 DOI: 10.1186/s12935-015-0221-1.
  2. Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. (2018) Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin 68, 394-424 DOI: 10.3322/caac.21492. Chen M, Chen C,Shen Z, Zhang X, Chen Y, Lin F, Ma X, Zhuang C, Mao Y, Gan H, Chen P, Zong X, Wu R. (2017). Extracellular pH is a biomarker enabling detection of breast cancer and liver cancer using CEST MRI. Oncotarget. 8, 45759- 45767 DOI: 10.18632/oncotarget.17404. Chen Y, Chen CH, Tung PY, Huang SH, Wang SM. (2009). An acidic extracellular pH disrupts adherens junctions in HepG2 cells by Src kinases-dependent modification of E-cadherin. J Cell Biochem,851-859
  3. DeBerardinis RJ, Sayed N, Ditsworth D, Thompson CB. (2008). Brick by brick: metabolism and tumor cell growth. Curr Opin Genet Dev. 18, 54-61 DOI: 10.1016/j.gde.2008.02.003 Estrella V, Chen T, Lloyd M, Wojtkowiak J, Cornnell HH, Ibrahim-Hashim A, Bailey K, Balagurunathan Y, Rothberg JM, Sloane BF, Johnson J, Gatenby RA, Gillies RJ et al. (2013). Acidity generated by the tumor microenvironment drives local invasion. Cancer Res. 73, 1524-1535 DOI: 10.1016/j.gde.2008.02.003 Feng Q, Shen Y, Fu Y, Muroski ME, Zhang P, Wang Q, Xu C, Lesniak MS, Li G, Cheng Y.(2017). Self-assembly of gold nanoparticles shows microenvironment-mediated dynamic switching and enhanced brain tumor targeting. Theranostics, 1875-1889 DOI: 10.7150/thno.18985. Fukamachi T, Ikeda S, Wang X, Saito H, Tagawa M, Kobayashi H. (2013). Gene expressions for signal transduction under acidic conditions. Genes, 65-85
  4. Gatenby RA, Gillies RJ. (2008). A microenvironmental model of carcinogenesis. Nat Rev Cancer, 56-61
  5. Gatenby RA, Gillies RJ.( 2004). Why do cancers have high aerobic glycolysis? Nat Rev Cancer 4, 891-899
  6. Gatenby RA, Gawlinski ET, Gmitro AF, Kaylor B, Gillies RJ.( 2006). Acid-mediated tumor invasion: a multidisciplinary study. Cancer Res, 5216-5223
  7. Hamaguchi R, Okamoto T, Sato M, Hasegawa M, Wada H. (2017). Effects of an alkaline diet on EGFR-TKI therapy in EGFR mutation-positive NSCLC. Anticancer Res, 37, 5141-5145
  8. Hao G, Xu ZP, Li L. (2018). Manipulating extracellular tumour pH: an effective targ