SavedAdd your journal

Journals / Flora İnfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi / 2001 / Cilt: 6 - Sayı: 1

Investigation of extended spectrum and chromosomal beta-lactamases in nosocomial gram-negative bacteria

Nozokomiyal gram-negatif bakterilerde genişlemiş spektrumlu ve kromozomal beta-laktamaz varlığının araştırlması

Özlem TÜNGER
Sivrel Ayşe ARISOY
Beril ÖZBAKKALOĞLU
Hörü GAZi
Pages
37–41
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Rates of stabile derepressed mutant was also determined 20.0% in Citrobacter spp, 14.3% in Enterobacter spp. and 12.1% in P. aeruginosa.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Determination of resistance rates due to extended spectrum beta-lactamases (ESBL) and inducible beta-lactamases in nosocomial gram-negative bacteria is important both for the successful management of the patient and the survey of antibiotic resistance patern within the hospital.
📊
Key Findings & Data:IBL activity was detected as 48.5% in P. aeruginosa and 19.0% in Enterobacter spp. while there was ESBL activity in 41.7%, 13.4%, 4.8% of K. pneumoniae, E. coli and Enterobacter spp, respectively.
💡
Conclusion & Impact:Rates of stabile derepressed mutant was also determined 20.0% in Citrobacter spp, 14.3% in Enterobacter spp. and 12.1% in P. aeruginosa.
🔬Design:Survey & Empirical Study
📍Scope:Comprehensive Sample
⚙️Variables:Mikrobiyal duyarlılık testleri · İlaç direnci · mikrobiyal

Abstract

Determination of resistance rates due to extended spectrum beta-lactamases (ESBL) and inducible beta-lactamases in nosocomial gram-negative bacteria is important both for the successful management of the patient and the survey of antibiotic resistance patern within the hospital. In this study ESBL and inducible beta-lactamase activity in two hundred thirty-nine, gram-negative bacteria isolated from nosocomial infections in Celal Bayar University Hospital during four years were examined. ESBL and inducible beta-lactamase activity were investigated by double disk synergy and direct induction tests, respectively. The bacteria resistant to broad spectrum cephalosporins, aztreonam, cefoxitin, beta-lactamase inhibitors and sensitive to imipenem were accepted as stabile derepressed mutant. IBL activity was detected as 48.5% in P. aeruginosa and 19.0% in Enterobacter spp. while there was ESBL activity in 41.7%, 13.4%, 4.8% of K. pneumoniae, E. coli and Enterobacter spp, respectively. Rates of stabile derepressed mutant was also determined 20.0% in Citrobacter spp, 14.3% in Enterobacter spp. and 12.1% in P. aeruginosa.

Özet

Hastane infeksiyonu etkeni gram-negatif bakterilerde genişlemiş spëktrumlu beta-laktamaz (GSBL) ve indüklenebilir beta-laktamaz (İBL) üretimine bağlı antibiyotik direnç oranlarının belirlenmesi hem tedavi başarısı hem de hastane direnç paterninin bilinmesi açısından önemlidir. Bu çalışmada, Celal Bayar Üniversitesi Hastanesi'nde 4 yıllık süre içinde soyutlanan 239 nozokomiyal gram-negatif bakteride GSBL ve indüklenebilir beta-laktamaz varlığı araştırılmıştır. GSBL saptamak için çift disk sinerji, İBL saptamak için direkt indüksiyon testi kullanılmıştır. Geniş spektrumlu sefalosporinler, aztreonam, sefoksitin ve beta-laktamaz inhibitörlerine dirençli, imipeneme duyarlı bakteriler ise stabil dereprese mutant olarak kabul edilmiştir. Klebsiella pneumoniae kökenlerinin %41.7'si, Escherichia coli kökenlerinin %13.4'ü ve Enterobacter spp. kökenlerinin %4.8'inde GSBL, Pseudomonas aeruginosa kökenlerinin %48.5'i, Enterobacter spp. kökenlerinin %19,0'unda İBL üretimi saptanmış, Citrobacter spp. kökenlerinin %20.0'si, Enterobacter spp. kökenlerinin %14.3'ü, P. aeruginosa kökenlerinin % 12. 1'inin ise stabil dereprese mutant olduğu belirlenmiştir.

🔖

Cite This Article

TÜNGER, Ö., ARISOY, S. A., ÖZBAKKALOĞLU, B., GAZi, H. (2001). Investigation of extended spectrum and chromosomal beta-lactamases in nosocomial gram-negative bacteria. Flora İnfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi, Cilt: 6 - Sayı: 1, 37–41. https://ajindex.com/articles/216144

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (13)

  1. Livermore DM. Beta-lactamases in laboratory and clini-cal resistance. Clin Microbiol Rev 1995
  2. Bush K, Jacoby GA, Medeiros AA. A functional classifi-cation scheme for beta-lactamases and its correlationwith molecular structure. Antimicrob Agents Chemother1995
  3. Katsanasis GP, Spargo J, Ferraro MJ, Sutton L, JacobyGA. Detection ofKlebsiella pneumoniaeand Escheric-hia coli strains producing extended spectrum beta-lacta-mases. J Clin Microbiol 1994
  4. Sanders CC, Sanders WE. Beta-lactam resistance ingram-negative bacteria: Global trends and clinical im-pact. Clin Infect Dis 1992
  5. Quinn JP. Clinical significance of extended-spectrumbeta-lactamases. Eur J Clin Microbiol Infect Dis 1994
  6. 13(Suppl 1):39-42. 6. Jarvis WR. Preventing the emergence of multidrug-resis-tant microorganisms through antimicrobial use controls:The complexity of the problem. Infect Control Hosp Epi-demiol 1996
  7. 5 7. Garner JS, Jarwis WR, Emory TG, Horan TC, HughesJM. CDC definitions for nosocomial infections. Am J In-fect Control 1988
  8. Koneman EW, Allen SD, Jonda WM, SchreckenbergerPC, Winn WC. Color Atlas and Textbook of DiagnosticMicrobiology, 5thed. Philadelphia: JB Lippincott Co,1997. 9. National Committee for Clinical Laboratory Standards:Performance standards for antimicrobial disk susceptibi-lity tests, 9thed. Approved Standard M100-S9. NationalCommittee for Clinical Laboratory Standards, Pennsyl-vania, 1999. 10. Legrand P, Fournier G, Bure F, et al. Detection of exten-ded spectrum beta-lactamases in Enterobacteriaceae infour French hospitals. Eur J Clin Microbiol Infect Dis1989
  9. Philippon A, Labia R, Jacoby G. Extended spectrumbeta-lactamases. Antimicrob Agents Chemother 1989
  10. Sirot J. Detection of extended spectrum plasmid medi-ated beta-lactamases by disk diffusion. Clin Microbiol andInfect 1996
  11. 2(Suppl 11):35-9. 13. Bal Ç. Beta-laktamaz testleri ve rutinde kullanımları. TürkMikrobiyoloji Cemiyeti Antibiyotik Duyarlılık Testleri’ninStandardizasyonu (ADTS) Çalışma Grubu. AntibiyotikDuyarlılık Testlerinin Standardizasyonu Toplantısı 11-12Nisan 1997, İstanbul. 101-111. 14. Gülay Z, Abacıoğlu YH, Yuluğ N. Çift disk sinerji yönte-minde diskler arası uzaklığın sonuca etkisi. İnfeksiyonDerg 1995
  12. Akata F. Gram-negatif bakterilerde beta-laktamaz tiplerive antibiyogramdan beta-laktamaz tipini tayin etmedekullanılabilecek yöntemler. İnfeksiyon Derg 1997
  13. Jacoby GA. Genetics