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Journals / Flora İnfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi / 2001 / Cilt: 6 - Sayı: 3

Resistance of hospital originated, ESBL (+) 50 Klebsiella pneumoniae strains to some antibiotics and the importance of the distance between discs in determining ESBL

Hastane kökenli GSBL yapan 50 Klebsiella pneumoniae suşunun bazı antibiyotiklere direnç oranları ve GSBL belirlenmesinde disklerarası mesafenin önemi

Şükran YAVUZDEMİR
A. Derya AYSEV
Haluk GÜRİZ
Pages
196–200
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Of 28 strains 10 (30.9%) were resistant to cefoxitin.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Resistance to some antibiotics were investigated by Kirby-Bauer disc-diffusion method in extended spectrum beta-lactamase (ESBL) positive 50 Klebsiella pneumoniae strains isolated from patients.
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Key Findings & Data:ESBL activity was found to be positive in 39 (78%), and 46 (92%) strains when the distance between disc centers were 30 and 20 mm respectively.
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Conclusion & Impact:Of 28 strains 10 (30.9%) were resistant to cefoxitin.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Klebsiella pneumoniae · Antibiyotikler · Mikrobiyal duyarlılık testleri

Abstract

Resistance to some antibiotics were investigated by Kirby-Bauer disc-diffusion method in extended spectrum beta-lactamase (ESBL) positive 50 Klebsiella pneumoniae strains isolated from patients. ESBL activity was found to be positive in 39 (78%), and 46 (92%) strains when the distance between disc centers were 30 and 20 mm respectively. Synergy was observed at 16 mm (center to center) in 4 (8%) strains. Resistance to amikacine 25 (50%), gentamicine 33 (66%), netilmicine 39 (78%), tobramycine 43 (86%), trimethoprim/sulfamethoxazole 36 (72%), tetracycline 28 (56%), ciprofloxacine 14 (28%), ofloxacine 14 (28%), norfloxacine 14 (28%), cefepim 18 (36%), ticarcillin/clavulanic acid 38 (76%), piperacillin/tazobactam 14 (28%) and meropenem 3 (6%) were found respectively. All strains were susceptible to imipenem and colistine. Of 28 strains 10 (30.9%) were resistant to cefoxitin.

Özet

Hastanede yatan hastaların çeşitli klinik örneklerinden soyutlanan, genişlemiş spektrumlu beta-laktamaz (GSBL) yapan 50 Klebsiella pneumoniae suşunun bazı antibiyotiklere duyarlılıkları Kirby-Bauer disk-difüzyon yöntemiyle araştırıldı. GSBL varlığı çift disk sinerji testi disk merkezleri arası uzaklık 30 mm ve 20 mm olarak uygulandığında sırasıyla 39 (%78) ve 46 (%92) suşta pozitif bulundu. Dört (%8) suşta ise yalnızca uzaklık 16 mm'de iken sinerji gözlendi. Dirençli suş sayısı ve oranları sırasıyla amikasin 25 (%50), gentamisin 33 (%66), nedimisin 39 (%78), tobramisin 43 (%86), trimetoprim/sulfametoksazol 36 (%72), tetrasiklin 28 (%56), siprofloksasin 14 (%28), ofloksasin 14 (%28), norfloksasin 14 (%28), sefepim 18 (%36), tikarsilin/klavulanik asit 38 (%76), piperasilin/tazobaktam 14 (%28) ve meropenem 3 (%6) olarak belirlendi, imipenem ve kolistine direnç belirlenmedi. Sefoksitine ise 28 suştan 10 (%30.9)'u dirençliydi.

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Cite This Article

YAVUZDEMİR, Ş., AYSEV, A. D., GÜRİZ, H. (2001). Resistance of hospital originated, ESBL (+) 50 Klebsiella pneumoniae strains to some antibiotics and the importance of the distance between discs in determining ESBL. Flora İnfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi, Cilt: 6 - Sayı: 3, 196–200. https://ajindex.com/articles/215373

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References (12)

  1. Podschun R, Ullman U. Klebsiella spp. as nosocomialpathogens: Epidemiology, taxonomy, typing methodsand pathogenicity factors. Clin Microb Rev 1998
  2. Swenson JM, Hindler JA, Peterson LR. Special phe-notypic methods for antibacterial resistance. In: MurrayPR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH (eds).Manual of Clinical Microbiology. 7thed. WashingtonDC, 1999:1569. 3.Quinn JP. Clinical significance of extended-spectrum be-ta-lactamases. Eur J Clin Microbiol Infect Dis 1994
  3. 13(Suppl 1):39-42. 4.Bush K. Is it important to identify extended-spectrum be-ta-lactamase-producing isolates? Eur J Clin Microb InfectDis 1996
  4. Gazouli M, Kaufmann ME, Tzelepi E, Dimopoulou H,Paniara O, Tzouvelekis LS. Study of an outbreak of ce-foxitin-resistant Klebsiella pneumoniae in a general hos-pital. J Clin Microb 1997
  5. Sanders CC, Barry AL, Washington JA, et al. Detectionof extended-spectrum-ß-lactamase-producing membersof the family Enterobacteriaceae with the vitek ESBLtest. J Clin Microb 1996
  6. Gülay Z, Abacıoğlu YH, Yuluğ N. Çift disk sinerji yönte-minde diskler arası uzaklığın sonuca etkisi. İnfeksiyonDerg 1995
  7. National Committee for Clinical Laboratory Standards(NCCLS). Performance Standards for Antimicrobial Sus-ceptibility Testing
  8. Ninth Informational Supplement. M2,1999. 9.Gülay Z, Amyes SGB, Yuluğ N. Genişletilmiş spektrumlubeta-laktamaz tanımlanmasında çift disk sinerji testi veizoelektrik odaklama yöntemi ile elde edilen sonuçlarınkarşılaştırılması. İnfeksiyon Derg 1999
  9. Jarlier V, Nicolas MH, Fournier G, Philippon A. Exten-ded broad spectrum ß-lactamases conferring transferableresistance to newer ß-lactam agents in Enterobacteriace-ae: Hospital prevalence and susceptibility patterns. RevInfect Dis 1988
  10. Legrand P, Fournier G, Bure A, et al. Detection of Ex-tended broad-spectrum beta-lactamases in Enterobacteri-acea in Four French Hospitals. Eur J Clin Microbiol In-fect Dis 1989
  11. Bauernfeind A, Phillippon A, Redjeb SB, Fournier G,Hassen AB. New plasmid-borne ß-lactamases. Epidemi-ology of extended spectrum ß-lactamases. Infection1989
  12. Gür D. Gram-negatif bakterilerde yeni genişlemiş spekt-rumlu beta-laktamazlar. Eraksoy H, Yenen OŞ (editörler).İnfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Kitabı. No-bel Tıp Kitabevleri, 2000:265-70. 14. Gültekin M, Öğünç D, Günseren F, Çolak D, Kırbaş İ,Mamıkoğlu L. Hastane infeksiyonu etkeni Klebsiellapneumon