Cell death due to methamphetamine in primary oligodendrocyte culture

Conclusions: These results suggest that neurotoxic effects that are observed in chronic mediamphetamine users may be partly due to the toxic effect of methamphetamine on oligodendrocyte.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: The neurotoxic effect of methamphetamine which is a psyhcostimulant drug has been established by in vitro and in vivo studies.

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Key Findings & Data:For this purpose cultures have been exposed to the various concentrations of methamphetamine (10-1000 $\mu$M with various incubation time (24-96 hours).

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Conclusion & Impact:Conclusions: These results suggest that neurotoxic effects that are observed in chronic mediamphetamine users may be partly due to the toxic effect of methamphetamine on oligodendrocyte.

🔬Design:Research Article

📍Scope:Comprehensive Sample

⚙️Variables:Apoptoz · Hayvanlar · yenidoğan

Abstract

Objective: The neurotoxic effect of methamphetamine which is a psyhcostimulant drug has been established by in vitro and in vivo studies. In this study, the cytotoxic effect of mediamphetamine on oligodendrocytes has been investigated. Material and Method: The cytotoxic effect of metamphetamine was determined by lactate dehydrogenase (LDH) test in neonatal Wistar rat oligodendrocyte cultures. The apoptosis inducing effect of methamphetamine was evaluated by apostain immunfluorescence staining method. For this purpose cultures have been exposed to the various concentrations of methamphetamine (10-1000 $\mu$M with various incubation time (24-96 hours). Results LDH measures show that mediamphetamine leads dose and time-dependent oligodendrocyte death. Apostain method points that methamphetamine results in apoptotic oligodendrocyte death especially at low (10 $\mu$M and high (1000 $\mu$M doses. Conclusions: These results suggest that neurotoxic effects that are observed in chronic mediamphetamine users may be partly due to the toxic effect of methamphetamine on oligodendrocyte.

Özet

Amaç: Psikostimülan bir ilaç olan metamfetaminin nörotoksik etkisi in vitro ve in vivo çalışmalarla kanıtlanmıştır. Bu çalışmada ise metamfetaminin yenidoğan Wistar sıçan oligodendrosit kültürlerinde sitotoksik etkisi araştırıldı. Gereç ve Yöntem: Metamfetaminin sitotoksik etkisi laktat dehidrogenaz (LDH) testiyle, apoptoz indükleyici etkisi ise apostain immünfloresan boyama yöntemiyle araştırıldı. Bu amaçla kültürler değişen konsantrasyonlarda (10-1000 $\mu$M arası) ve değişik enkübasyon sürelerinde (24-96 saat arası) metamfetamiııe maruz bırakıldı. Bulgular: LDH ölçümleri metamfetaminin doza ve zamana bağımlı olarak oligodendrosit ölümüne yol açtığını göstermektedir. Apostain yöntemi ise metamfetaminin düşük (10 $\mu$M) ve yüksek (1000 $\mu$M) dozlarda daha belirgin apoptoza neden olduğunu işaret etmektedir. Sonuç: Bu sonuçlar kronik metamfetamin kullanıcılarında gözlenen nörotoksik etkilerin kısmen bu maddenin oligodendrositler üzerine toksik etkisine de bağlı olabileceğini düşündürmektedir.

Keywords

Apoptoz Hayvanlar yenidoğan Sıçan Wistar Metamfetamin Modeller hayvan teorik Sitotoksisite immünolojik Apoptosis Animals Newborn Rats Methamphetamine Models Animal Theoretical Cytotoxicity Immunologic

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Cite This Article

GENÇ, K. K., GENÇ, Ş., SÖNMEZ, Ü., YILMAZ, O., SÖNMEZ, A., TUĞYAN, K., ERGÜR, B., BULDAN, Z. (2002). Cell death due to methamphetamine in primary oligodendrocyte culture. Dokuz Eylül Üniversitesi Tıp Fakültesi Dergisi, Cilt: 16 - Sayı: 3, 141–147. https://ajindex.com/articles/213397

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