SavedAdd your journal

Journals / Bezmiâlem Science / 2021 / Cilt: 9 - Sayı: 1

Gemifloksasinin 4-bromometil-7-metoksikumarin Belirteci Kullanılarak Farmasötik Preparatlarda HPLC ile Analizi

A Liquid Chromatographic Analysis of Gemifloxacin in Pharmaceutical Preparations Using 4-bromomethyl-7- methoxycoumarin Reagent

Cem ÖNAL
Pages
41–45
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Sonuç: Geliştirilen metod, ilaç maddesin farmasötik preparatlardaki analizine başarıyla uygulanmştır.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Amaç: Bu çalışmada gemifloksasinin 4-bromometil-7- metoksikumarin belirteci ve dibenz-18-taç-6 eter katalizör varlığında farmasötik preparatlarda yüksek performanslı sıvı kromatografisi ile analizi gerçekleştirilmiştir.
📊
Key Findings & Data:Yöntemler: Türevlendirme işlemi sonucu oluşan bileşiğin eksitasyon dalga boyu λext. =325 nm ve emisyon dalga boyu λem. =390 nm olarak bulunmuştur.
💡
Conclusion & Impact:Sonuç: Geliştirilen metod, ilaç maddesin farmasötik preparatlardaki analizine başarıyla uygulanmştır.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Amaç: Bu çalışmada gemifloksasinin 4-bromometil-7- metoksikumarin belirteci ve dibenz-18-taç-6 eter katalizör varlığında farmasötik preparatlarda yüksek performanslı sıvı kromatografisi ile analizi gerçekleştirilmiştir. Yöntemler: Türevlendirme işlemi sonucu oluşan bileşiğin eksitasyon dalga boyu λext. =325 nm ve emisyon dalga boyu λem. =390 nm olarak bulunmuştur. Optimum reaksiyon şartları dikkatlice çalışıldı. Kromatografik ayırmalar, 250x4,6 mm, 5 µm I.D C18 kolonda, asetonitril-0,05 M sulu amonyum asetat (pH=5,0) (70:30, v/v) mobil fazında, 1 mL/dak akış hızında gerçekleştirilmiştir. Bulgular: Kalibrasyon eğrisi 10-200 ng.mL-1 aralığında doğrusal bulunmuştur. Ortalama geri kazanım %100,32 ve bağıl standart sapma değerleri %2’nin altında bulunmuştur. Sonuç: Geliştirilen metod, ilaç maddesin farmasötik preparatlardaki analizine başarıyla uygulanmştır.

Özet

Objective: In this study, analysis of gemifloxacin in pharmaceutical preparations was performed in the presence of 4-bromomethyl-7- methoxycoumarin reagent and dibenzo-18-crown-6 ether catalyst, by high-performance liquid chromatography. Methods: The excitation wavelength of the compound formed as a result of the derivatization process was found as λext. =325 nm and the emission wavelength as λem. =390 nm. Optimum reaction conditions were carefully studied. Chromatographic separations were performed in a 150 cm x4.6 mm, 5 µm I.D C18 column, and the mobile phase consisting of acetonitrile: 0.05 M aqueous ammonium acetate (pH=5.0) (70:30, v/v) under flow rate of 1.0 mL/min. Results: The calibration curve was found to be linear in the range of 10-200 ng.mL-1. Average recovery was 100.32% and relative standard deviation values were below 2%. Conclusion: The method developed has been successfully applied in the analysis of the drug substance in pharmaceutical preparations.

🔖

Cite This Article

ÖNAL, C. (2021). Gemifloksasinin 4-bromometil-7-metoksikumarin Belirteci Kullanılarak Farmasötik Preparatlarda HPLC ile Analizi. Bezmiâlem Science, Cilt: 9 - Sayı: 1, 41–45. https://ajindex.com/articles/212329

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (14)

  1. Budavari S. The Merck Index,Merck and Co. Whitehouse Station, NJ, USA: 13th ed, 2001
  2. Völgyi G, Vizserálek G, Takács-Novák K, Avdeef A, Tam KY. Predicting the exposure and antibacterial activity of fluoroquinolones based on physicochemical properties. Eu J Pharm Sci 2012;47:21-7
  3. Oh JI, Paek KS, Ahn MJ, Kim MY, Hong CY, Kim IC, et al. In vitro and in vivo evaluations of LB20304, a new fluoronaphthyridone. Antimicrob Agents Chemother 1996;40:1564-8
  4. Hohl AF, Frei R, Punter V, Graevenitz A, Knapp C, Washington J, et al. International multicenter investigation of LB20304, a new fluoronaphthyridone. Clin Microbiol Infect 1998;4:280-4
  5. Kepekci Tekkeli SE, Önal A. Spectrofluorimetric methods for the determination of gemifloxacin in tablets and spiked plasma samples. J Fluoresc 2011;21:1001-7
  6. Youssef NF, Bebawy LI. Spectrofluorimetric methods for the determination of gemifloxacin mesylate and cefamandole nafate in bulk powder and pharmaceutical preparations. Bulletin of the Faculty of Pharmacy 2006;44:215-27
  7. Rote AR, Pingle SP. Reverse phase-HPLC and HPTLC methods for determination of gemifloxacin mesylate in human plasma. J Chromatogr B 2009;877:3719-23
  8. Al-Hadiya BM, Khady AA, Mostafa GA. Validated liquid chromatographic-fluorescence method for the Quantitation of gemifloxacinin human plasma. Talanta 2010;83:110-6
  9. Kaiser M, Grünspan LD, Costa TD, Tasso L. Reversed phase liquid chromatography method with fluorescence detection of gemifloxacin in rat plasma and its application to the pharmacokinetic study. J Chromatogr B 2011;879:3639-44
  10. Nageswara RR, Naidu ChG, Guru PK, Padiya R, Agwane SB. Determination of gemifloxacin on dried blood spots by hydrophilic interaction liquid chromatography with fluorescence detector: application to pharmacokinetics in rats. Biomed Chromatogr 2012;26:1534-42
  11. Doyle E, Fowles SE, McDonnell DF, White SA Rapid determination of gemifloxacin in human plasma by high performance liquid chromatography-tandem mass spectrometry. J Chromatogr B 2000:746:191-8
  12. Radi AE, Khafagy A, El-Shobaky A, El-Mezayen H. Anodic Voltammetric determination of gemifloxacin using screenprinted carbon electrode J Pharm Anal 2013;3:132-6
  13. Elbashir, AA. Saad, B. Ali, ASM, Al-Azzam, KMM, Aboul-Enein HY, Validated stability indicating assay of gemifloxacin and lomefloxacin in tablet formulations by capillary electrophoresis J Liq Chromatogr Relat Technol 2008;31:1465-77
  14. Zacharis CK, Raikos N, Giouvalakis N, Tsou