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Journals / Dicle Tıp Dergisi / 2016 / Cilt: 43 - Sayı: 1

Koroner Arter Hastalığında MTHFR ve MMP-9 Genetik Variantları

MTHFR and MMP-9 Genetic Variants in Coronary Artery Disease

MERAL URHAN KÜÇÜK
AYŞEGÜL BAYRAMOĞLU
HALİL İBRAHİM GÜLER
Semire GÖÇMEN UZUN
Yunusq KUÇUKKAYA
Okay ABACI
ABDULLAH ARPACI
MUSTAFA ÇAĞATAY KORKMAZ
Pages
50–56
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

However, TT genotype was determined to be very rare in Southeast Anatolia

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: Coronary artery disease (CAD) is a multifacto- rial disease that influenced by both genetic and environ- mental factors.
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Key Findings & Data:In the present study we investigated the both independent and synergistically effects of matrix me- talloproteinase (MMP) -1562 C/T and methylenetetrahy- drofolate reductase (MTHFR) 677 C/T polymorphisms on the CAD occurrence.
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Conclusion & Impact:However, TT genotype was determined to be very rare in Southeast Anatolia
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: Coronary artery disease (CAD) is a multifacto- rial disease that influenced by both genetic and environ- mental factors. Single nucleotide polymorphisms (SNPs) in the candidate genes produce susceptibility to such mul- tifactorial diseases. Therefore, investigations of SNPs, in the genes that may play role in etiopathogenesis of CAD, become crucial. In the present study we investigated the both independent and synergistically effects of matrix me- talloproteinase (MMP) -1562 C/T and methylenetetrahy- drofolate reductase (MTHFR) 677 C/T polymorphisms on the CAD occurrence. Methods: In total 217 individuals, 109 coronary artery disease patients and 108 healthy controls were exam- ined. We determined the genotypes for MMP-9 -1562 C/T and MTHFR 677 C/T polymorphisms by polymerase chain reaction (PCR)-restriction fragment length polymor- phism (RFLP). Results: We found no statistically significant differences between genotypes and allelic frequencies of both MMP- 9 -1562 C/T and MTHFR 677 C/T polymorphisms and CAD (p>0.05). No TT homozygous genotype was found in any of groups for MMP9 -1562 C/T polymorphism. However, while C allele and CC genotype was found to be highly, TT genotype was found to be very rare for both polymorphisms in Southeastern Anatolia. Conclusion: We have found no associations between MMP9 -1562 C/T and MTHFR 677 C/T polymorphisms and coronary artery disease. However, TT genotype was determined to be very rare in Southeast Anatolia

Özet

Amaç: Koroner arter hastalığı (KAH), hem genetik hem de çevresel faktörlerden etkilenen çok faktörlü bir hasta- lıktır. Aday genlerdeki tek nükleotid polimorfizmleri (SNP) bu tür multifaktöryel hastalıklara yatkınlığa neden olurlar. Bu yüzden, KAH etiyopatogenezinde rol oynayan genler- de SNPlerin araştırılması, önemli hale gelir. Bu çalışma- da, KAH oluşumu üzerinde, matriks metalloproteinaz-9 (MMP9) -1562 C/T ve metilentetrahidrofolat redüktaz (MTHFR) 677 C/T polimorfizmlerinin bağımsız ve siner- jistik etkileri araştırıldı. Yöntemler: 109 koroner arter hastası ve 108 sağlıklı kontrol olmak üzere toplam 217 birey incelendi. MTHFR 677 C/T ve MMP-9 -1562 C/T polimorfizmleri için genotip- ler polimeraz zincir reaksiyonu (PCR)- restriksiyon frag- manı uzunluk polimorfizmi (RFLP) ile belirlendi. Bulgular: KAH ile MMP-9 -1562 C/T ve MTHFR - 677 C/T polimorfizmlerinin genotipleri ve alel frekansları arasında istatistiksel olarak anlamlı bir farklılık olmadığı bulundu. (p> 0.05) MMP9 -1562 C/T polimorfizmi için TT homo- zigot genotipi hiç bir grupta bulunmadı. Bununla birlikte, Güneydoğu Anadolu Bölgesinde C aleli ve CC genotipi her iki polimorfizm için hakim iken, TT genotipi ise çok nadir olarak bulundu. Sonuç: MTHFR 677 C/T ve MMP9 -1562 C/T polimor- fizmleri ile koroner arter hastalığı arasında ilişki bulunma- dı. Ancak Güneydoğu Anadolu bölgesinde TT genotipinin çok nadir olduğu belirlendi.

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Cite This Article

KÜÇÜK, M. U., BAYRAMOĞLU, A., GÜLER, H. İ., UZUN, S. G., KUÇUKKAYA, Y., ABACI, O., ARPACI, A., KORKMAZ, M. Ç. (2016). Koroner Arter Hastalığında MTHFR ve MMP-9 Genetik Variantları. Dicle Tıp Dergisi, Cilt: 43 - Sayı: 1, 50–56. https://ajindex.com/articles/209745

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References (14)

  1. Morgan AR, Zhang B, Tapper W, et al. Haplotypic analysis of the MMP-9 gene in relation to coronary artery disease. J Mol Med 2003;81:321-326
  2. Danzig V, Míková B, Kuchynk P, et al. Levels of Circulating Biomarkers at Rest and after Exercise in Coronary Artery Disease Patients Physiol Res 2010;59:385-392
  3. Lusis AJ. Atherosclerosis. Nature 2000;407:233-241
  4. Zhi H., Wang H, Ren L, et al. Functional polymorphisms of matrix metallopeptidase-9 and risk of coronary artery dis- ease in a Chinese population. Mol Biol Rep 2010;37:13-20
  5. Bayramoglu A, Urhan-Kucuk M, Guler HI, et al. Is there any genetic predisposition of MMP-9 gene C1562T and MTHFR gene C677T polymorphisms with essential hyper- tension? Cytotechnology 2015;67:115-22
  6. Tayebjee MH, Lip GY, Tan KT, et al. Plasma matrix metal- loproteinase-9, tissue inhibitor of metalloproteinase-2, and CD40 ligand levels in patients with stable coronary artery disease. Am J Cardiol 2005;96:339-345
  7. Nannı S, Melandrı G, Hanemaaıjer R, et al. Matrix metal- loproteinases in premature coronary atherosclerosis: in- fluence of inhibitors, inflammation, and genetic polymor- phisms. Transl Res 2007;149:137-144
  8. Kai H, Ikeda H, Yasukawa H, et al. Peripheral blood lev- els of matrix metalloproteases-2 and -9 are elevated in pa- tients with acute coronary syndromes. J Am Coll Cardiol 1998;32:368-372
  9. Opstad TB, Pettersen AA, Weiss TW, et al. Genetic variation, gene-expression and circulating levels of matrix etallopro- teinase-9 in patients with stable coronary artery disease. Clin Chim Acta 2012;413:113-120
  10. Zhang B, Ye S, Herrmann SM, et al. Functional polymor- phism in the regulatory region of gelatinase B gene in re- lation to severity of coronary atherosclerosis. Circulation 1999;99:1788-1794
  11. Abilleira S, Bevan S., Markus HS. The role of genetic vari- ants of matrix metalloproteinases in coronary and carotid atherosclerosis. J Med Genet 2006;43:897-901
  12. Saedi M, Vaisi-Raygani A, Khaghani S, et al. Matrix me- talloproteinase-9 functional promoter polymorphism 1562C>T increased risk of early-onset coronary artery dis- ease. Mol Biol Rep 2012;39:555-562
  13. İzmirli M, Aldemir Ö, Gögebakan B, et al. The studies about diseases concerning with contemplated MTHFR 677 C>T polymorphism. Dicle Medical Journal 2014;41:244-256
  14. Dikmen M. Molecular Biology of Methylenetetrahydrofo- late Reductase (MTHFR) Enzyme and Its Association with Diseases. The Medical Journal of Kocatepe 2004;5:9-16