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Journals / KBB-Forum / 2003 / Cilt: 2 - Sayı: 1

Aminoglikozit içeren kulak damlalarının neden olduğu ototoksisiteden bir spin trap'le (PBN) korunma: Kronik etkiler

Protection against aminoglycoside otic drop-induced ototoxicity by a spin trap (PBN): Chronic effects

Ümit TUNCEL
Erdinç AYGENÇ
William CLERICI
Journal
KBB-Forum
Pages
17–23
DOI
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⚡ TL;DR(Too Long; Didn't Read)

Conclusion: These findings suggest that free radical mediated events are partially responsible for, and that DPOAEs are very sensitive to, AD induced chronic ototoxicity in guinea pigs.

📌 3 Key Takeaways

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Objective & Hypothesis:Objectives: To investigated the long-term spin trap protection against aminoglycoside otic drop-induced ototoxicity.
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Key Findings & Data:Material and Methods: We evaluated the ability of intraperitoneal α-phenyl-tert-butyl-nitrone (PBN) or 0.9% saline applications to reduce the chronic effects of topical aminoglycoside otic drops (AD) or artificial perilymph (PER) treatment on cochlear microphonic (CM), compound action potential (CAP) and 2F1-F2 distortion product otoacoustic emission (DPOAEs) sensitivities in three groups of guinea pigs. (n=5/group): a) saline+PER (controls), b) saline+AD and c) PBN+AD.
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Conclusion & Impact:Conclusion: These findings suggest that free radical mediated events are partially responsible for, and that DPOAEs are very sensitive to, AD induced chronic ototoxicity in guinea pigs.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Modeller · teorik · Polimiksin B

Abstract

Objectives: To investigated the long-term spin trap protection against aminoglycoside otic drop-induced ototoxicity. Material and Methods: We evaluated the ability of intraperitoneal &#945;-phenyl-tert-butyl-nitrone (PBN) or 0.9% saline applications to reduce the chronic effects of topical aminoglycoside otic drops (AD) or artificial perilymph (PER) treatment on cochlear microphonic (CM), compound action potential (CAP) and 2F1-F2 distortion product otoacoustic emission (DPOAEs) sensitivities in three groups of guinea pigs. (n=5/group): a) saline+PER (controls), b) saline+AD and c) PBN+AD. Results: Relative to control values, saline+AD group mean CM and CAP values were severely degraded at all frequencies (p<.05). PBN+AD group mean losses were significantly (p<.05) smaller than those of the saline+AD group for CAP and CM at 8, 12, 16 and 24 kHz, indicating that PBN provided protection against topical AD induced damage. Mean 2F1-F2 DPOAE values decreased to below background levels at all frequencies in groups receiving ADs, irrespective of PBN treatment. Conclusion: These findings suggest that free radical mediated events are partially responsible for, and that DPOAEs are very sensitive to, AD induced chronic ototoxicity in guinea pigs.

Özet

Amaç: Aminoglikozit içeren kulak damlalarının (AD) neden olduğu ototoksisitenin engellenmesinde spin trapl’erin uzun süreli koruyucu etkisini araştırmak. Yöntem ve Gereçler: Topikal aminoglikozid kulak damlaları (AD) ve yapay perilenf(PER) tedavisinin kohlear mikrofonik (CM), birleşik aksiyon potansiyeli (CAP) ve 2F1-F2 distorsiyon product otoakustik emisyon(DPOAE) sensitiviteleri üzerindeki kronik etkilerinin intraperitoneal α-phenyl-tert-butyl-nitrone (PBN) ve %0.9 NaCl (saline) uıygulaması ile azalıp azalmadığını 3 guinea pig grubu üzerinde araştırdık (n=5/grup):a)saline + PER;b)saline+AD; c) PBN +AD. Bulgular: Kontrol değerleriyle karşılaştırıldığında,saline+AD grubunun ortalama CM ve CAP değerleri bütün frekanslarda ileri derecede azalmıştı (p<.05). CAP ve CM için, PBN+AD grubundaki ortalama kayıplar saline+AD grubundan 8,12,16 ve 24 kHz’de belirgin olarak daha azdı, ve ve bu durum PBN’nin topikal AD’nin oluşturduğu hasarı önlediğini gösteriyordu. Bütün frekanslardaki ortalama 2F1-F2 DPOAE değerleri, AD alan bütün gruplarda PBN tedavisinden bağımsız olarak ilk değerlerinin altına indi. Sonuç: Bu bulgular, serbest radikaller aracılığıyla oluşan olayların guinea pig’lerde AD tarafından meydana getirilen ototoksisiteden kısmen sorumlu olduğunu ve DPOAE’lerin bu ototoksisiteye karşı çok duyarlı olduğunu desteklemektedir.

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Cite This Article

TUNCEL, Ü., AYGENÇ, E., CLERICI, W. (2003). Aminoglikozit içeren kulak damlalarının neden olduğu ototoksisiteden bir spin trap'le (PBN) korunma: Kronik etkiler. KBB-Forum, Cilt: 2 - Sayı: 1, 17–23. https://ajindex.com/articles/209396

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