Journals / KONURALP TIP DERGİSİ / 2018 / Cilt: 10 - Sayı: 3

The Invitro Sensitivity of Bacterial Conjunctivitis Agents to Routinely Used Antibiotics

Bakteriyel Konjonktivit Etkenlerinin Rutinde Kullanılan Antibiyotiklere İnvitro Duyarlılıkları

Pages
369–372
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

The sensitivity percentages of the examined antibiotics were found to be netilmicin 93.3%, chloramphenicol 92.5%, tobramycin 90.0%, gentamicin 88.2%, moxifloxacin 84.3%, ofloxacin 80.9%, tetracycline 75.0%, ciprofloxacin 70.5% and lomefloxacin 63.3%.Conclusion: The results of this study showed that rather than using broad spectrum fluoroquinolones in empirical treatment of patients with bacterial conjuctivitis, the first-line use of aminoglycoside-derived antibiotics would have a positive effect on the treatment process.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: The most commonly seen ocular infection worldwide is bacterial conjunctivitis.
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Key Findings & Data:In the current study, it was aimed to find an effective empirical treatment by isolating bacterial conjunctivitis agents and determining the antibiotic sensitivity percentages in invitro conditions.Methods: Culture samples were taken from the mucopurulent discharge of 65 patients diagnosed with bacterial conjunctivitis.
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Conclusion & Impact:The sensitivity percentages of the examined antibiotics were found to be netilmicin 93.3%, chloramphenicol 92.5%, tobramycin 90.0%, gentamicin 88.2%, moxifloxacin 84.3%, ofloxacin 80.9%, tetracycline 75.0%, ciprofloxacin 70.5% and lomefloxacin 63.3%.Conclusion: The results of this study showed that rather than using broad spectrum fluoroquinolones in empirical treatment of patients with bacterial conjuctivitis, the first-line use of aminoglycoside-derived antibiotics would have a positive effect on the treatment process.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Bakteriyel konjonktivit · invitro antibiyotik duyarlılığı · Ampirik Tedavi

Abstract

Objective: The most commonly seen ocular infection worldwide is bacterial conjunctivitis. It is widespread practice to use broad spectrum empirical treatment for bacterial conjunctivitis without production of the agent micro-organism or performing antibiotic sensitivity tests. In the current study, it was aimed to find an effective empirical treatment by isolating bacterial conjunctivitis agents and determining the antibiotic sensitivity percentages in invitro conditions.Methods: Culture samples were taken from the mucopurulent discharge of 65 patients diagnosed with bacterial conjunctivitis. The samples were taken with a swab stick into transport medium, then seeded and the bacteria colonies produced were identified using a fully automated BD Phoenix system (Becton Dickinson , USA). Each of the produced bacteria were evaluated using netilmicin, tobramycin, ofloxacin, tetracycline, chloramfenicol, gentamicin, lomefloxacin, moxifloxacin, and ciprofloxacin discs with the Kirby-Bauer disc diffusion method.Results: The 65 patients with bacterial conjuntivitis comprised 30 females and 35 males, age between 21 and 86 (average: 48.23 ±10.47). The agent bacteria was isolated in 46 (70.7%) cases. Of the produced bacteria, 86.9% were gram positive cocci, and of these, coagulase negative staphylococci was the most frequently isolated. The sensitivity percentages of the examined antibiotics were found to be netilmicin 93.3%, chloramphenicol 92.5%, tobramycin 90.0%, gentamicin 88.2%, moxifloxacin 84.3%, ofloxacin 80.9%, tetracycline 75.0%, ciprofloxacin 70.5% and lomefloxacin 63.3%.Conclusion: The results of this study showed that rather than using broad spectrum fluoroquinolones in empirical treatment of patients with bacterial conjuctivitis, the first-line use of aminoglycoside-derived antibiotics would have a positive effect on the treatment process.

Özet

Amaç: Dünyada en sık görülen oküler enfeksiyon, bakteriyel konjonktivitlerdir. Bakteriyel konjonktivitlerde etken mikroorganizma üretilmeden ve antibiyotik duyarlılık testleri yapılmadan geniş spektrumlu ampirik tedavi kullanımı yaygındır. Biz bu çalışmada, bakteriyel konjonktivit etkenlerini izole ederek invitro şartlarda antibiyotik duyarlılık yüzdelerini saptayarak etkin ampirik tedaviyi bulmayı amaçladık.Gereç ve Yöntem: Bakteriyel konjonktivit tanısı almış 65 hastanın mukopürülan akıntılarından eküvyon çubuğu ile transport besiyeri içerisine kültür örnekleri alındı. Kültür örneklerinin ekimleri yapılarak üreyen bakteri kolonileri BD Phoenix (Becton Dickinson, USA) tam otomatize sistem kullanılarak tanımlandı. Üreyen bakterilerin herbirine; netilmisin, tobramicin, ofloksasin, tetrasiklin, kloramfenikol, gentamisin, lomefloksasin, moksifloksasin, siprofloksasin diskleri ile Kirby-Bauer disk difüzyon yöntemi uygulandı.Bulgular: Çalışmaya dahil edilen yaşları 21 ile 86 (yaş ortalaması: 48.23 ±10.47) aralığında bakteriyel konjonktivit tanısı almış 30 kadın 35 erkek toplam 65 bakteriyel konjonktivitli hastanın 46’sında (%70,7) etken bakteri izole edildi. Üreyen bakterilerin %86,9’u gram pozitif koklar olup bunlardan da koagülaz negatif stafilokoklar en sık izole edildi. Çalışılan antibiyotiklerin duyarlılık yüzdeleri sırasıyla netilmisin %93,3, kloramfenikol %92,5, tobramisin %90,0, gentamisin %88,2 moksifloksasin %84,3, ofloksasin %80,9, tetrasiklin75,0, siprofloksasin %70,5 lomefloksasin %63,3 olarak bulundu.Sonuç: Yapmış olduğumuz çalışmamızın sonuçlarıyla bakteriyel konjonktivitli hastaların ampirik tedavisinde geniş spekturumlu florokinolonların yerine aminoglikozid türevi antibiyotiklerin ilk sırada kullanılmasının tedavi sürecinde olumlu etki yaratacağı kanaatindeyiz.

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Cite This Article

ÇAKMAKLIOĞULLARI, M., ÇAKMAKLIOĞULLARI, E. K. (2018). The Invitro Sensitivity of Bacterial Conjunctivitis Agents to Routinely Used Antibiotics. KONURALP TIP DERGİSİ, Cilt: 10 - Sayı: 3, 369–372. https://ajindex.com/articles/202705

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