Journals / Turkish Journal of Botany / 2000 / Cilt: 24 - Sayı: 2

Histology of Somatic Embryogenesis in Cultured Leaf Explants of Pistachio (Pistacia veraL)

Pages
91–96
Publication Date
2000-02-01
DOI
—
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

These results clearly demonstrate that the induction and development of somatic embryos from leaf explants of pistachio were from single epidermal or subepidermal cells.

📌 3 Key Takeaways

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Objective & Hypothesis:The histology of somatic embryo initiation and development in pistachio ( Pistacia were L.) embryogenic masses (EMS) derived from leaf explants was examined using light microscopy.
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Key Findings & Data:Explants with somatic embryos at different developmental stages were fixed for histological examination, cut into 10 µm thick sections, stained with hematoxylin-eosin and observed microscopically.
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Conclusion & Impact:These results clearly demonstrate that the induction and development of somatic embryos from leaf explants of pistachio were from single epidermal or subepidermal cells.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Pistacia vera L. · leaf explants · histology.

Abstract

The histology of somatic embryo initiation and development in pistachio ( Pistacia were L.) embryogenic masses (EMS) derived from leaf explants was examined using light microscopy. Explants with somatic embryos at different developmental stages were fixed for histological examination, cut into 10 µm thick sections, stained with hematoxylin-eosin and observed microscopically. The histological examination showed that the two types of cell clusters induced originated from explants and were morphologically different. These results clearly demonstrate that the induction and development of somatic embryos from leaf explants of pistachio were from single epidermal or subepidermal cells.

Özet

The histology of somatic embryo initiation and development in pistachio ( Pistacia were L.) embryogenic masses (EMS) derived from leaf explants was examined using light microscopy. Explants with somatic embryos at different developmental stages were fixed for histological examination, cut into 10 µm thick sections, stained with hematoxylin-eosin and observed microscopically. The histological examination showed that the two types of cell clusters induced originated from explants and were morphologically different. These results clearly demonstrate that the induction and development of somatic embryos from leaf explants of pistachio were from single epidermal or subepidermal cells.

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Cite This Article

ONAY, A. (2000). Histology of Somatic Embryogenesis in Cultured Leaf Explants of Pistachio (Pistacia veraL). Turkish Journal of Botany, Cilt: 24 - Sayı: 2, 91–96. https://ajindex.com/articles/197978

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