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Journals / Genel Tıp Dergisi / 2015 / Cilt: 25 - Sayı: 2

Effects of exercise training and coenzyme Q10 supplementation on oxidative stress and antioxidant defense markers in heart tissue of aged rats

Yaşlı sıçanlarda antrenman ve koenzim Q10 takviyesinin kalp dokusunda oksidatif stres ve antioksidan savunma belirteçleri üzerine etkileri

MUAZ BELVİRANLI
NİLSEL OKUDAN
AYŞE NURTEN ÖZDEMİR
Mehmet ÖZ
Pages
52–57
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: Although aging, CoQ10 supplementation and exercise training have limited effect on lipid peroxidation, DNA damage and non-enzymatic antioxidant levels, they decreased antioxidant enzyme activity in the heart tissue

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of this study was to investigate the effects of exercise training and/or coenzyme Q10 (CoQ10) supplementa- tion on oxidative stress and antioxidant defense changes in heart tissue of aged rats.
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Key Findings & Data:Material and methods: Twenty-seven young (3-months of age) and 27 aged (20-months of age) Wistar male rats were used in the study.
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Conclusion & Impact:Conclusion: Although aging, CoQ10 supplementation and exercise training have limited effect on lipid peroxidation, DNA damage and non-enzymatic antioxidant levels, they decreased antioxidant enzyme activity in the heart tissue
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The aim of this study was to investigate the effects of exercise training and/or coenzyme Q10 (CoQ10) supplementa- tion on oxidative stress and antioxidant defense changes in heart tissue of aged rats. Material and methods: Twenty-seven young (3-months of age) and 27 aged (20-months of age) Wistar male rats were used in the study. Each group was further divided into four subgroups: Control, training, CoQ10 and CoQ10+training. The training program consisted of swimming exercise one hour per day, five days a week, for eight weeks. The CoQ10 were given at a daily dose of 300 mg.kg-1 of body weight five days/week via oral gavage. Heart tissues were taken under anesthesia then rats were sacrificed by servical dislocation. Malondialdehyde (MDA), 8-hydroxy-2-de- oxyguanosine (8-OHdG), glutathione (GSH) levels and superoxide dismutase (SOD) and catalase (CAT) activities were analyzed in samples.Results: There was no significant difference among the groups with respect to MDA, 8-OHdG and GSH levels. SOD and CAT activities were lower in the young CoQ10 and in the CoQ10 plus training groups than the young control, young training and all aged groups. Conclusion: Although aging, CoQ10 supplementation and exercise training have limited effect on lipid peroxidation, DNA damage and non-enzymatic antioxidant levels, they decreased antioxidant enzyme activity in the heart tissue

Özet

Bu çalışmanın amacı, yaşlı sıçanlarda antrenman ve/veya koenzim Q10 (CoQ10) desteğinin kalp dokusunda oksidatif stres ve antioksidan savunma değişiklikleri üzerine etkilerini incelemekti. Gereç ve yöntem: Çalışmada 27 genç (3 aylık) ve 27 yaşlı (20 aylık) Wistar erkek sıçan kullanıldı. Her bir grup; kontrol, antrenman, CoQ10 ve CoQ10+antrenman olmak üzere dörder alt gruba ayrıldı. Antrenman gruplarındaki sıçanlara 8 hafta boyunca, haftada 5 gün, günde 1 saat yüzme egzersizi yaptırıldı. CoQ10 desteği alan sıçanlara 8 hafta boyunca günde 300 mg/kg CoQ10 oral olarak verildi. Sıçanlar, anestezi altında kalp dokusu örnekleri alındıktan sonra servikal dislokasyonla sakrifiye edildi. Alınan örneklerde malondialdehit (MDA), 8-hidroksi-2-deoksiguanozin (8-OHdG), glutatyon (GSH) seviyeleri ve süperoksit dismutaz (SOD) ve katalaz (CAT) aktiviteleri ölçüldü. Bulgular: Gruplar arasında MDA, 8-OHdG ve GSH seviyelerinde herhangi bir fark yoktu. SOD ve CAT aktivitesi genç CoQ10 ve CoQ10+antrenman gruplarında ve tüm yaşlı gruplarda, genç kontrol ve genç antrenman gruplarından düşüktü. Sonuç: Yaşlanma, CoQ10 takviyesi ve antrenman, kalp dokusunda lipid peroksidasyonu, DNA hasarı ve enzimatik olmayan antioksidan seviyeleri üzerine sınırlı etkiye sahipken, antioksidan enzim aktivitesini azaltır

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Cite This Article

BELVİRANLI, M., OKUDAN, N., ÖZDEMİR, A. N., ÖZ, M. (2015). Effects of exercise training and coenzyme Q10 supplementation on oxidative stress and antioxidant defense markers in heart tissue of aged rats. Genel Tıp Dergisi, Cilt: 25 - Sayı: 2, 52–57. https://ajindex.com/articles/192596

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