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Journals / Adli Tıp Bülteni / 1997 / Cilt: 2 - Sayı: 3

Spermatozoa isolation and ABO(H) group typing with proteinase K on semen contaminated vaginal material

Semen kontamine vajinal materyalde proteinaz K ile spermatozoa izolasyonu ve ABO(H) grup tayini

Demirçin Sema KARAGÖZ
Beyhan EGE
Pages
118–123
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It was calculated that ratio of obtaining a true result was 82.67 % with Proteinase K treated samples and 86 % with nontreated samples.

📌 3 Key Takeaways

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Objective & Hypothesis:Physical and gynecological examinations and detection of spermatozoa in vaginal material are the procedures used to prove the sexual crimes as rape, sexual assault, sodomy and adultary cases.
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Key Findings & Data:In this study, the applicability of an absorbtion elution technique with proteinase K treatment is investigated to increase the reliability of ABO(H) group typing on semen contaminated vaginal swabs.
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Conclusion & Impact:It was calculated that ratio of obtaining a true result was 82.67 % with Proteinase K treated samples and 86 % with nontreated samples.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Physical and gynecological examinations and detection of spermatozoa in vaginal material are the procedures used to prove the sexual crimes as rape, sexual assault, sodomy and adultary cases. Besides these examinations, assessment of genetic markers in the vaginal material is necessary to identify the offender in cases of sexual assault with un¬ known perpetrator. In this study, the applicability of an absorbtion elution technique with proteinase K treatment is investigated to increase the reliability of ABO(H) group typing on semen contaminated vaginal swabs. It was seen that after Proteinase K treatment only spermatozoa remain in vaginal material and blood group obtained in conclusive reflects, only the antigenic structure of spermatooza. It was calculated that ratio of obtaining a true result was 82.67 % with Proteinase K treated samples and 86 % with nontreated samples.

Özet

Seksüel suçlar adı verilen ırza geçme, tasaddi, livata ve zina olaylarında suçun kanıtlanması için genel fizik muayene, jinekolojik bakı ve vajinal materyalin spermato¬zoa varlığı açısından incelenmesi gereklidir, Saldırının gerçekleştiği ancak failin belli olmadığı olgularda bunlara ek olarak, suçlunun kimliğini belirleyebilmek amacıyla vaji¬nal materyalde genetik işaretler incelenmelidir. Bu çalışma¬da semen kontamine vajinal sürüntülerde ABO grup tiplemesinde güvenilirliği arttırmak' için absorbsiyon elüs-y-on ile birlikte proteinaz K kullanımının uygulanabilirliği araştırıldı. Proteinaz K eklendikten sonra vajinal materyal içinde saciece spermatozoidlerin kaldığı ve sonuç olarak el¬de edilen kan grubunun sadece spermatozoanın antijenik yapısını yansıttığı görüldü. Absorbsiyon-elüsyon tekniği ile Proteinaz K kullanılmadan değerlendirilen örneklerde doğru sonuç elde edilme oranı % 86, proteinaz K kullanılan örneklerde % 82.67 olarak bulundu.

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Cite This Article

KARAGÖZ, D. S., EGE, B. (1997). Spermatozoa isolation and ABO(H) group typing with proteinase K on semen contaminated vaginal material. Adli Tıp Bülteni, Cilt: 2 - Sayı: 3, 118–123. https://ajindex.com/articles/189831

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