SavedAdd your journal

Journals / Turkish Journal of Chemistry / 2008 / Cilt: 32 - Sayı: 4

One-Step Synthesis of Hyaluronic Acid-Based (Sub)micron Hydrogel Particles: Process Optimization and Preliminary Characterization

Nurettin Sahiner And Xinqiao JIA
Pages
397–409
Publication Date
2008-04-01
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These HA-based hydrogel particles are promising candidates for use in drug delivery.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Hyaluronic acid (HA)-based (sub)micron hydrogel particles were synthesized by crosslinking virgin HA with divinyl sulfone (DVS) in sodium bis(2-ethylhexyl)sulfosuccinate (AOT) reverse micelle systems under basic conditions.
📊
Key Findings & Data:The use of a cosurfactant, 1-heptanol (1-HP), had a profound effect on the reaction kinetics and the particle morphology.
💡
Conclusion & Impact:These HA-based hydrogel particles are promising candidates for use in drug delivery.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Hyaluronic acid · (Sub)micron particles · Drug delivery

Abstract

Hyaluronic acid (HA)-based (sub)micron hydrogel particles were synthesized by crosslinking virgin HA with divinyl sulfone (DVS) in sodium bis(2-ethylhexyl)sulfosuccinate (AOT) reverse micelle systems under basic conditions. Experimental parameters including HA molecular weight, solution concentration, and reaction time were systematically varied in order to obtain well defined hydrogel particles. The use of a cosurfactant, 1-heptanol (1-HP), had a profound effect on the reaction kinetics and the particle morphology. The resulting particles exhibit negative charges on their surfaces. In vitro biocompatibility study indicates that these particles do not cause severe death to the cultured fibroblasts. These HA-based hydrogel particles are promising candidates for use in drug delivery.

Özet

Hyaluronic acid (HA)-based (sub)micron hydrogel particles were synthesized by crosslinking virgin HA with divinyl sulfone (DVS) in sodium bis(2-ethylhexyl)sulfosuccinate (AOT) reverse micelle systems under basic conditions. Experimental parameters including HA molecular weight, solution concentration, and reaction time were systematically varied in order to obtain well defined hydrogel particles. The use of a cosurfactant, 1-heptanol (1-HP), had a profound effect on the reaction kinetics and the particle morphology. The resulting particles exhibit negative charges on their surfaces. In vitro biocompatibility study indicates that these particles do not cause severe death to the cultured fibroblasts. These HA-based hydrogel particles are promising candidates for use in drug delivery.

🔖

Cite This Article

JIA, N. S. A. X. (2008). One-Step Synthesis of Hyaluronic Acid-Based (Sub)micron Hydrogel Particles: Process Optimization and Preliminary Characterization. Turkish Journal of Chemistry, Cilt: 32 - Sayı: 4, 397–409. https://ajindex.com/articles/185053

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (27)

  1. O. Olabisi, L.M., Robeson, M. T., Shaw, “Polymer-Polymer Miscibility”, Academic Press: New York, 1979.
  2. G.G.Guilbault, R.F. Chen, F. Govindjee; G.bPapageorgiou, E. Rabinowitch, E.L. Wehry, “In Practical Fluorescence”, G.G. Guilbault, Ed.; Marcel Dekker: New York, Chap. 1. 1973.
  3. E.A. Hegazy, A.M. Dessouki, N.B. El-Assy, N.M. El-Sawy, M.A.A. El-Ghaffar, J. Appl. Polym. Sci. 30, 1976 (1992).
  4. C. Kiani, L. Chen, Y.J. Wu, A.J. Yee, B.B.Yang, Cell Res. 12,19-32, (2002).
  5. R. Barbucci, S. Lamponi, A. Borzacchiello, L. Ambrosio, M. Fini, P. Torricelli, R. Giardino, Biomaterials,, 4503-4513, (2002).
  6. Y. Luo, G.D. Prestwich, Bioconjugate Chem. 12, 1085-1088, (2001).
  7. X.Z. Shu, G.D. Prestwich, “Therapeutic biomaterials from chemically modiŞed hyaluronan.”, In: H. Garg, C. Hales, editors. Chemistry and Biology of Hyaluronan. New York: Elsevier; p 475, 2004.
  8. J. B. Leach, K.A. Bivens, C.W. Patrick, C.E. Schmidt, Biotech. Bioeng. 82, 578-589 (2003).
  9. K. S. Masters, D. N. Shah, L.A. Leinwand, K. S. Anseth, Biomater. 26, 2517-2525 (2005).
  10. A. Khademhosseini, G. Eng, J. Yeh, J. Fukuda, J. Blumling, R. Langer, J.A. Burdick, J. Biomed. Mater. Res. 79A, 522-532 (2006).
  11. G. Lepperdinger, C. Fehrer, S. Reitinger, “Biodegradation of hyaluronan.” In: H.G. Garg, C.A. Hales, editors. Chemistry and Biology of Hyaluronan. Oxford: Elsevier p 71, 2004.
  12. X. Jia, Y.Yeo, R.J. Clifton, T. Jiao, D.S. Kohane, J.B. Kobler, S.M. Zeitels, R. Langer, Biomacromol., 7, 3344 (2006).
  13. K. Landfester, Adv. Mater., 13, 765-768 (2001).
  14. B.K. Paul, R.K.Mitra, J. Coll. Interf. Sci. 288, 261-279 (2005).
  15. K.H. Bae, J.J.Yoon, T.G. Park, Biotech. Prog. 22, 297-302 (2006).
  16. E. Esposito, E. Menegatti, R. Cortesi, Int. J. Pharm. 288, 35-49 (2005).
  17. S.K. Hahn, S. Jelacic, R.V. Maier, P.S. Stayton, A.S. Hoffman, J. Biomater. Sci. Polym. Ed. 15, 1111-1119 (2004).
  18. K.W. Marshall, Curr. Opin. Rheumatol. 12, 468-474 (2000).
  19. N. Bellamy, J. Campbell, V. Robinson, T. Gee, R. Bourne, G. Wells, Cochrane Database of Systematic Reviews, 2005.
  20. Y.H.Yun, D.J.Goetz, P.Yellen, W.L. Chen, Biomater. 25, 147-157 (2004).
  21. N. Sahiner, Coll. Polym. Sci. 4, 413-421 (2007).
  22. N. Sahiner, Coll. Polym. Sci. 285:283-292 (2006).
  23. B. Baruah, J. M. Roden, M. Sedgwick, N.M. Correa, D.C. Crans, N.E. Levinger, J. Am. Chem. Soc. 128, 12765 (2006).
  24. S.C. Rizzi, J.A. Hubbell, Biomacromol. 6, 1226-1238 (2005).
  25. Y. Luo, K.R. Kirker, G.D. Prestwich, J. Cont. Rel. 69, 169-184 (2000).
  26. P. Bulpitt, D. Aeschlimann, J. Biomed. Mater. Res. 47, 152-169 (1999).
  27. D. Ruhela, K. Riviere