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Journals / Turkish Journal of Botany / 2008 / Cilt: 32 - Sayı: 3

Induction of Embryogenic Tissue from Immature Zygotic Embryos in Pinus nigra J.F.Arnold subsp. nigra var. caramanica (Loudon) Businsky

Zeynep ÖZKURT
Tolga YILDIRIM
Sertaç ÖNDE
Zeki KAYA
Pages
179–183
Publication Date
2008-03-01
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Overall initiation frequencies of EC in the study were 0.92% in 2004 and 1.96% in 2005; tree values ranged from 0.0% to 7.32%.

📌 3 Key Takeaways

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Objective & Hypothesis:Embryogenic cultures (EC) of Anatolian black pine (Pinus nigra J.F.Arnold subsp. nigra var. caramanica (Loudon) Businsky) were initiated from immature pre-cotyledonary zygotic embryos sampled weekly from 16 different trees in 2 consecutive years.
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Key Findings & Data:Douglas-fir cotyledon revised (DCR) medium supplemented with 13.6 µM of 2,4-D and 2.2 µM of BAP was used for initiation and maintenance of EC.
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Conclusion & Impact:Overall initiation frequencies of EC in the study were 0.92% in 2004 and 1.96% in 2005; tree values ranged from 0.0% to 7.32%.
🔬Design:Research Article
📍Scope:2004 Verileri
⚙️Variables:Pinus nigra subsp. nigra var. caramanica · somatic embryogenesis · initiation

Abstract

Embryogenic cultures (EC) of Anatolian black pine (Pinus nigra J.F.Arnold subsp. nigra var. caramanica (Loudon) Businsky) were initiated from immature pre-cotyledonary zygotic embryos sampled weekly from 16 different trees in 2 consecutive years. Douglas-fir cotyledon revised (DCR) medium supplemented with 13.6 µM of 2,4-D and 2.2 µM of BAP was used for initiation and maintenance of EC. Overall initiation frequencies of EC in the study were 0.92% in 2004 and 1.96% in 2005; tree values ranged from 0.0% to 7.32%. Overall, 0.38% and 0.62% of the initial explants were converted into established cell lines in 2004 and 2005, respectively.

Özet

Embryogenic cultures (EC) of Anatolian black pine (Pinus nigra J.F.Arnold subsp. nigra var. caramanica (Loudon) Businsky) were initiated from immature pre-cotyledonary zygotic embryos sampled weekly from 16 different trees in 2 consecutive years. Douglas-fir cotyledon revised (DCR) medium supplemented with 13.6 µM of 2,4-D and 2.2 µM of BAP was used for initiation and maintenance of EC. Overall initiation frequencies of EC in the study were 0.92% in 2004 and 1.96% in 2005; tree values ranged from 0.0% to 7.32%. Overall, 0.38% and 0.62% of the initial explants were converted into established cell lines in 2004 and 2005, respectively.

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Cite This Article

ÖZKURT, Z., YILDIRIM, T., ÖNDE, S., KAYA, Z. (2008). Induction of Embryogenic Tissue from Immature Zygotic Embryos in Pinus nigra J.F.Arnold subsp. nigra var. caramanica (Loudon) Businsky. Turkish Journal of Botany, Cilt: 32 - Sayı: 3, 179–183. https://ajindex.com/articles/183578

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References (6)

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