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Journals / Turkish Journal of Botany / 2013 / Cilt: 37 - Sayı: 4

Molecular characterisation of the genus Papaver section Oxytona using ISSR markers

Tuğba GÜRKÖK
Elif KAYMAK
Gülşen BOZTEPE
Mesut KOYUNCU
İskender PARMAKSIZ
Pages
644–650
Publication Date
2013-04-01
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, the ISSR marker system can be used for the classification of section Oxytona.

📌 3 Key Takeaways

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Objective & Hypothesis:Papaver bracteatum Lindl., P. orientale L., and P. pseudo-orientale (Fedde) Medw., species of section Oxytona Bernh. in the genus Papaver L., are found widely in the native flora of Turkey.
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Key Findings & Data:In this study, 20 ISSR primers have been studied in 180 accessions collected from 5 different regions of Turkey.
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Conclusion & Impact:In conclusion, the ISSR marker system can be used for the classification of section Oxytona.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Key words: Papaver · Oxytona · ISSR

Abstract

Papaver bracteatum Lindl., P. orientale L., and P. pseudo-orientale (Fedde) Medw., species of section Oxytona Bernh. in the genus Papaver L., are found widely in the native flora of Turkey. They are commercially important for their high alkaloid content. Because genus Papaver section Oxytona has similar morphological characters, it is not easy to distinguish these species. We used the inter-simple sequence repeat (ISSR) molecular marker system to determine the molecular characterisation of this section. In this study, 20 ISSR primers have been studied in 180 accessions collected from 5 different regions of Turkey. A total of 82 bands were obtained, of which 80 were polymorphic. Average genetic distance was found to be 0.35, the Shannon index was 0.50, and the polymorphism rate was 96.97%. The primer AT10 had the smallest number of bands, which was 3, while the primers AT19 and AT3 had the greatest number of bands, which was 5. In conclusion, the ISSR marker system can be used for the classification of section Oxytona.

Özet

Papaver bracteatum Lindl., P. orientale L., and P. pseudo-orientale (Fedde) Medw., species of section Oxytona Bernh. in the genus Papaver L., are found widely in the native flora of Turkey. They are commercially important for their high alkaloid content. Because genus Papaver section Oxytona has similar morphological characters, it is not easy to distinguish these species. We used the inter-simple sequence repeat (ISSR) molecular marker system to determine the molecular characterisation of this section. In this study, 20 ISSR primers have been studied in 180 accessions collected from 5 different regions of Turkey. A total of 82 bands were obtained, of which 80 were polymorphic. Average genetic distance was found to be 0.35, the Shannon index was 0.50, and the polymorphism rate was 96.97%. The primer AT10 had the smallest number of bands, which was 3, while the primers AT19 and AT3 had the greatest number of bands, which was 5. In conclusion, the ISSR marker system can be used for the classification of section Oxytona.

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Cite This Article

GÜRKÖK, T., KAYMAK, E., BOZTEPE, G., KOYUNCU, M., PARMAKSIZ, İ. (2013). Molecular characterisation of the genus Papaver section Oxytona using ISSR markers. Turkish Journal of Botany, Cilt: 37 - Sayı: 4, 644–650. https://ajindex.com/articles/183405

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