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Journals / Turkish Journal of Botany / 2019 / Cilt: 43 - Sayı: 1

Genetic characterization of Orobanche cumana populations from the Thrace region of Turkey using microsatellite markers

Behiye Banu BİLGEN
Ahmet Kubilay BARUT
Sefer DEMİRBAŞ
Pages
38–47
Publication Date
2019-01-11
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The information obtained from this study is valuable to provide a significant contribution to studies on the genetic structure, diversity, and race evolution of O. cumana and to the developed crop breeding studies and management strategies for controlling O. cumana infestation.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Orobanche cumana is a parasitic plant that can lead to advanced losses in yield of agricultural lands cultivating sunflower, depending on the sunflower varieties and the level of contamination.
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Key Findings & Data:A total of 23 alleles were determined for the analyzed 120 samples.
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Conclusion & Impact:The information obtained from this study is valuable to provide a significant contribution to studies on the genetic structure, diversity, and race evolution of O. cumana and to the developed crop breeding studies and management strategies for controlling O. cumana infestation.
🔬Design:Research Article
📍Scope:120 samples
⚙️Variables:Broomrape · Helianthus annuus · molecular marker analysis

Abstract

Orobanche cumana is a parasitic plant that can lead to advanced losses in yield of agricultural lands cultivating sunflower, depending on the sunflower varieties and the level of contamination. In our study, genetic diversity of six O. cumana populations from the Thrace region of Turkey was determined with the help of eight SSR (microsatellite) loci. All SSR loci were polymorphic. A total of 23 alleles were determined for the analyzed 120 samples. Allele number of each SSR locus ranged from two to six. Mean number of alleles (Na = 2.271), effective allele number (Ne = 1.667), Shannon?s information index (I = 0.547), and heterozygosity levels (Ho = 0.207 and He = 0.340) were calculated. A high proportion of the genetic variation (66%) was due to within-population variation and 34% of the diversity was due to among-population variations. Based on the UPGMA dendrogram and STRUCTURE analysis, there were two main clusters. Cluster I was classified into three groups containing four populations from the Kırklareli and Edirne regions. Two populations from Tekirdağ were in cluster II. The information obtained from this study is valuable to provide a significant contribution to studies on the genetic structure, diversity, and race evolution of O. cumana and to the developed crop breeding studies and management strategies for controlling O. cumana infestation.

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Cite This Article

BİLGEN, B. B., BARUT, A. K., DEMİRBAŞ, S. (2019). Genetic characterization of Orobanche cumana populations from the Thrace region of Turkey using microsatellite markers. Turkish Journal of Botany, Cilt: 43 - Sayı: 1, 38–47. https://ajindex.com/articles/182768

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