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Journals / Turkish Journal of Botany / 2020 / Cilt: 44 - Sayı: 1

Assessment of antioxidant system and enzyme/nonenzyme regulation related to ascorbate-glutathione cycle in ferulic acid-treated Triticum aestivum L. roots under boron toxicity

Ceyda ÖZFİDAN-KONAKÇI
Evren YILDIZTUGAY
Fevzi ELBASAN
Ayşegül YILDIZTUGAY
Mustafa KÜÇÜKÖDÜK
Pages
36–46
Publication Date
2020-01-07
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Consequently, FA eliminated the damage induced by B stress via the increased POX and the enzymes related to Asada-Halliwell pathway (AsA-GSH cycle) in wheat roots.

📌 3 Key Takeaways

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Objective & Hypothesis:Ferulic acid (FA; 3-methoxy-4-hydroxycinnamic acid) can eliminate stress-induced damage because of its ability to induce antioxidant activity under stress.
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Key Findings & Data:Plants were grown in hydroponic culture containing the combination or alone form of 25-75 µM FA and 4-8 mM B.
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Conclusion & Impact:Consequently, FA eliminated the damage induced by B stress via the increased POX and the enzymes related to Asada-Halliwell pathway (AsA-GSH cycle) in wheat roots.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Antioxidant system · ascorbate-glutathione cycle · boron stress

Abstract

Ferulic acid (FA; 3-methoxy-4-hydroxycinnamic acid) can eliminate stress-induced damage because of its ability to induce antioxidant activity under stress. The aim of this study was to identify the effects of FA on water status, antioxidant system, and lipid peroxidation in wheat (Triticum aestivum L.) roots exposed to boron (B) stress. Plants were grown in hydroponic culture containing the combination or alone form of 25-75 µM FA and 4-8 mM B. Stress significantly decreased growth (RGR), water content (RWC), proline content (Pro), and osmotic potential (YP). However, FA alleviated the decrease in RGR, RWC, and Pro content. Compared to the control groups, stress decreased the activities of superoxide dismutase (SOD), peroxidase (POX), catalase, and ascorbate peroxidase (APX), but an increase was only observed in glutathione reductase (GR) activity. Hydrogen peroxide (H2O2) content accumulated with B stress. Besides, a notable decrease was observed in the scavenging activity of hydroxyl radical (OH?); thus, wheat roots had high lipid peroxidation (thiobarbituric acid reactive substance content). In response to stress, FA triggered the activities of SOD, POX, and APX. Moreover, when FA was made present in stressed wheat roots, we observed the enhanced activities of dehydroascorbate reductase, and monodehydroascorbate reductase and dehydroascorbate contents which are related to ascorbate-glutathione cycle, so FA could maintain ascorbate (AsA) regeneration. However, when wheat roots were treated with stress, FA did not induce the regeneration of glutathione because of decline in GR activity. Due to successful elimination of H2O2 content, the exogenous application of FA alleviated B-induced lipid peroxidation in wheat. Consequently, FA eliminated the damage induced by B stress via the increased POX and the enzymes related to Asada-Halliwell pathway (AsA-GSH cycle) in wheat roots.

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Cite This Article

ÖZFİDAN-KONAKÇI, C., YILDIZTUGAY, E., ELBASAN, F., YILDIZTUGAY, A., KÜÇÜKÖDÜK, M. (2020). Assessment of antioxidant system and enzyme/nonenzyme regulation related to ascorbate-glutathione cycle in ferulic acid-treated Triticum aestivum L. roots under boron toxicity. Turkish Journal of Botany, Cilt: 44 - Sayı: 1, 36–46. https://ajindex.com/articles/182733

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