Journals / Turkish Journal of Botany / 2020 / Cilt: 44 - Sayı: 3

Biochemical and morphological responses to cadmium-induced oxidative stress in Cladophora glomerata

Pages
222–231
Publication Date
2020-05-01
DOI
—
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

Results indicated that this alga had wide tolerance to high cadmium concentrations, and the stress compounds in the alga with exposure of Cd2+ seemed to be parameters as a biomarker for metal-induced oxidative stress.

📌 3 Key Takeaways

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Objective & Hypothesis:This study aimed to assess the effect of cadmium Cd2+ concentrations on biovolume, pigments, malondialdehyde MDA , hydrogen peroxide, proline, total phenolic compounds, total protein, and total carbohydrate contents of Cladophora glomerata .
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Key Findings & Data:Cultivations of the alga 8.0 ± 0.1 g as fresh weight were exposed to 7.5, 15, 30, and 60 mg/L of Cd2+ ions in 500 mL flasks containing 400 mL of the medium on a shaker at 120 rpm for 7 days.
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Conclusion & Impact:Results indicated that this alga had wide tolerance to high cadmium concentrations, and the stress compounds in the alga with exposure of Cd2+ seemed to be parameters as a biomarker for metal-induced oxidative stress.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Biochemical response · cadmium · Cladophora glomerata

Abstract

This study aimed to assess the effect of cadmium Cd2+ concentrations on biovolume, pigments, malondialdehyde MDA , hydrogen peroxide, proline, total phenolic compounds, total protein, and total carbohydrate contents of Cladophora glomerata . Cultivations of the alga 8.0 ± 0.1 g as fresh weight were exposed to 7.5, 15, 30, and 60 mg/L of Cd2+ ions in 500 mL flasks containing 400 mL of the medium on a shaker at 120 rpm for 7 days. Results of Fourier transform infrared analyses indicated that the amide, anionic, and amino groups had significant rolesin the binding of Cd2+ on C. glomerata. The detrimental effects of the Cd2+ dose not only observed the morphology of the algal cell but also changed the biochemical compounds of C. glomerata. Growth gradually decreased when the alga was exposed to Cd2+ at 15 and 60 mg/L in comparison with the control. High Cd2+ ions concentrations decreased in chlorophyll-a from 14.27 mg/g in control to 9.97 mg/g at 60 mg/L Cd and protein content from 43.60 mg/g in control to 21.66 mg/g at 60 mg/L Cd in the treated cells compared to the control group, whereas they increased stress molecules e.g., MDA and proline as biomarkers in the response mechanisms of algae to Cd2+ exposure. Results indicated that this alga had wide tolerance to high cadmium concentrations, and the stress compounds in the alga with exposure of Cd2+ seemed to be parameters as a biomarker for metal-induced oxidative stress.

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Cite This Article

ÇELEKLİ, A., BULUT, H. (2020). Biochemical and morphological responses to cadmium-induced oxidative stress in Cladophora glomerata. Turkish Journal of Botany, Cilt: 44 - Sayı: 3, 222–231. https://ajindex.com/articles/182719

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References (12)

  1. Andosch A, Affenzeller MJ, Lütz C, Lütz-Meindl U (2012). A freshwater green alga under cadmium stress: ameliorating calcium effects on ultrastructure and photosynthesis in the unicellular model Micrasterias. Journal of Plant Physiology 169 (15): 1489-1500.
  2. Arief VO, Trilestari K, Sunarso J, Indraswati N, Ismadji S (2008). Recent progress on biosorption of heavy metals from liquids using low cost biosorbents: characterization, biosorption parameters and mechanism studies. Clean 36 (12): 937-962.
  3. Bates L, Waldren R, Teare I (1973). Rapid determination of free proline for water-stress studies. Plant and Soil 39 (1): 205-207.
  4. Bellinger E, Sigee D (2010). Introduction to Freshwater Algae. Freshwater Algae: Identification and Use As Bioindicators. Oxford, UK: John Wiley & Sons. Boughalleb F, Abdellaouı R, Mahmoudi M, Bakhshandeh E (2020). Changes in phenolic profile, soluble sugar, proline, and antioxidant enzyme activities of Polygonum equisetiforme in response to salinity. Turkish Journal of Botany 44: 25-35.
  5. Branco D, Lima A, Almeida SF, Figueira E (2010). Sensitivity of biochemical markers to evaluate cadmium stress in the freshwater diatom Nitzschia palea (Kützing) W. Smith. AquaticToxicology 99 (2):109-117.
  6. Cao DJ, Xie PP, Deng JW, Zhang HM, Ma RX et al. (2015). Effects of Cu2+ and Zn2+ on growth and physiological characteristics of green algae, Cladophora. Environmental Science and Pollution Research 22 (21): 16535-16541.
  7. Chen H, Chen J, Guo Y, Wen Y, Liu J et al. (2012). Evaluation of the role of the glutathione redox cycle in Cu (II) toxicity to green algae by a chiral perturbation approach. AquaticToxicology 120:19-26.
  8. Choudhary M, Jetley UK, Khan MA, Zutshi S, Fatma T (2007). Effect of heavy metal stress on proline, malondialdehyde, and superoxide dismutase activity in the cyanobacterium Spirulina platensis-S5. Ecotoxicology and Environmental Safety 66 (2): 204-209.
  9. Çelekli A, Gültekin E, Bozkurt H (2016). Morphological and biochemical responses of Spirogyra setiformis exposed to cadmium. Clean 44 (3): 256-262.
  10. Çelekli A, Kapı E, Soysal Ç, Arslanargun H, Bozkurt H (2017). Evaluating biochemical response of filamentous algae integrated with different water bodies. Ecotoxicology and Environmental Safety 142: 171-180.
  11. Çelekli A, Kapı M, Bozkurt H (2013). Effect of cadmium on biomass, pigmentation, malondialdehyde, and proline of Scenedesmus quadricauda var. longispina. Bulletin of Environmental Contamination and Toxicology 91 (5): 571-576.
  12. Dubois M, Gill