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Journals / Turkish Journal of Biology / 2010 / Cilt: 34 - Sayı: 3

The genotoxic and antigenotoxic effects of Aloe vera leaf extract in vivo and in vitro

Ahmet KAYRALDIZ
Ayşe Yavuz KOCAMAN
Eyyüp RENCÜZOĞULLARI
Pages
235–246
Publication Date
2010-03-01
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

AV was a weak mutagen in the TA98 strain of Salmonella typhimurium in the absence of S9mix; however, AV+NPD (4-nitro-o-phenylenediamine) and AV+SA (sodium azide) exhibited a synergism in increasing the number of revertants for the TA98 and TA100 strains in the absence of S9mix, respectively.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The genotoxic and antigenotoxic effects of Aloe vera leaf extract (AV) were investigated using the chromosome aberrations (CAs) test for the bone marrow cells of rats, sister chromatid exchanges (SCEs) and micronucleus (MN) and CAs tests for human lymphocytes, and the Ames Salmonella/microsome test system.
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Key Findings & Data:In the bone marrow cells of rats, AV extract significantly induced structural and total CAs at all concentrations and in all treatment periods.
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Conclusion & Impact:AV was a weak mutagen in the TA98 strain of Salmonella typhimurium in the absence of S9mix; however, AV+NPD (4-nitro-o-phenylenediamine) and AV+SA (sodium azide) exhibited a synergism in increasing the number of revertants for the TA98 and TA100 strains in the absence of S9mix, respectively.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Key words: Aloe vera · culture of human lymphocytes in vitro · bone marrow cells

Abstract

The genotoxic and antigenotoxic effects of Aloe vera leaf extract (AV) were investigated using the chromosome aberrations (CAs) test for the bone marrow cells of rats, sister chromatid exchanges (SCEs) and micronucleus (MN) and CAs tests for human lymphocytes, and the Ames Salmonella/microsome test system. In the bone marrow cells of rats, AV extract significantly induced structural and total CAs at all concentrations and in all treatment periods. In human peripheral lymphocytes, AV did not increase the mean SCE; however, it significantly induced the MN frequency and structural CAs. In addition, AV showed a cytotoxic effect by decreasing the replication index (RI), mitotic index (MI), and nuclear division index (NDI) in human lymphocytes and by decreasing the MI in the bone marrow cells of rats. AV did not decrease the genotoxicity or cytotoxicity of urethane (ethyl carbamate, EC) in the bone marrow cells of rats or in the mitomycin-C (MMC) in human lymphocytes. AV was a weak mutagen in the TA98 strain of Salmonella typhimurium in the absence of S9mix; however, AV+NPD (4-nitro-o-phenylenediamine) and AV+SA (sodium azide) exhibited a synergism in increasing the number of revertants for the TA98 and TA100 strains in the absence of S9mix, respectively.

Özet

The genotoxic and antigenotoxic effects of Aloe vera leaf extract (AV) were investigated using the chromosome aberrations (CAs) test for the bone marrow cells of rats, sister chromatid exchanges (SCEs) and micronucleus (MN) and CAs tests for human lymphocytes, and the Ames Salmonella/microsome test system. In the bone marrow cells of rats, AV extract significantly induced structural and total CAs at all concentrations and in all treatment periods. In human peripheral lymphocytes, AV did not increase the mean SCE; however, it significantly induced the MN frequency and structural CAs. In addition, AV showed a cytotoxic effect by decreasing the replication index (RI), mitotic index (MI), and nuclear division index (NDI) in human lymphocytes and by decreasing the MI in the bone marrow cells of rats. AV did not decrease the genotoxicity or cytotoxicity of urethane (ethyl carbamate, EC) in the bone marrow cells of rats or in the mitomycin-C (MMC) in human lymphocytes. AV was a weak mutagen in the TA98 strain of Salmonella typhimurium in the absence of S9mix; however, AV+NPD (4-nitro-o-phenylenediamine) and AV+SA (sodium azide) exhibited a synergism in increasing the number of revertants for the TA98 and TA100 strains in the absence of S9mix, respectively.

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Cite This Article

KAYRALDIZ, A., KOCAMAN, A. Y., RENCÜZOĞULLARI, E. (2010). The genotoxic and antigenotoxic effects of Aloe vera leaf extract in vivo and in vitro. Turkish Journal of Biology, Cilt: 34 - Sayı: 3, 235–246. https://ajindex.com/articles/182489

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References (14)

  1. Topaktas M, Rencuzogullari E, Ila HB. In vivo chromosomal aberrations in bone marrow cells of rats treated with Marshal. Mutation Res 371: 259-264, 1996. Evans HJ. Human peripheral blood lymphocytes for the analysis of chromosome aberrations in mutagen tests. In: Kilbey BJ, Legator M, Nichols W, Ramel C. eds. Handbook of Mutagenicity Test Procedures, 2nd ed. Elsevier Sciences Publishers; 1984, pp. 405-427.
  2. Perry PE, Thompson EJ. The methodology of sister chromatid exchanges. In: Kilbey BJ, Legator M, Nichols W, Ramel C. eds. Handbook of Mutagenicity Test Procedures, 2nd ed. Elsevier Sciences Publishers; 1984, pp. 495-529.
  3. Albertini RJ, Anderson D, Douglas GR et al. IPCS guidelines for the monitoring of genotoxic effects of carcinogens in humans. Mutation Res 463: 111-172, 2000.
  4. Speit G, Haupter S. On the mechanism of differential Giemsa staining of bromodeoxyuridine-substituted chromosomes. Hum Genet 70: 126-129, 1985.
  5. Roncada T, Vicentini VE, Mantovani MS. Possible modulating actions of plant extracts on the chromosome breaking activity of MMC and Ara-C in human lymphocytes in vitro. Toxicol In Vitro 18: 617-622, 2004. Mendelsohn ML. Antimutagenic effects in humans. Mutation Res 267: 257-264, 1992.
  6. Paz-y-Mino C, Bustamante G, Sanchez ME et al. Cytogenetic monitoring in a population occupationally exposed to pesticides in Ecuador. Environ Health Perspective 110: 1077- 1080, 2002.
  7. Mace J ML, Daskal Y, Wray W. Scanning-electron microscopy of chromosome aberrations. Mutation Res 52: 199-206, 1978. Fenech M. The in vitro micronucleus technique. Mutation Res 455: 81-95, 2000.
  8. Kirsch-Volders M, Sofuni T, Aardemac M. Report from the in vitro micronucleus assay working group. Mutation Res 540: 153-163, 2003.
  9. Redbook, Toxicological principles for the safety assessment of food ingredients, bacterial reverse mutation test. Office of Food Additive Safety, 2000.
  10. Maron DM, Ames BN. Revised method for the Salmonella mutagenicity test. Mutation Res 113: 173-215, 1983.
  11. Vogler BK, Ernst E. Aloe vera: a systematic review of its clinical effectiveness. Br J Gen Pract 49: 823-828, 1999.
  12. Heidemann A, Volkner W, Mengs U. Genotoxicity of aloeemodin in vitro and in vivo. Mutat Res 367: 123-133, 1996.
  13. Liberman DF, Schaefer FL, Fink RC et al. Mutagenicity of islandicin and chrysophanol in the Salmonella/microsome system. Appl Environ Microbiol 40: 476-479, 1980.
  14. NTP, National Toxicology Program. Toxicology and carcinogenesis studies of emodin (CAS NO. 518-82-1) feed studies in F344/