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Journals / Turkish Journal of Biology / 2008 / Cilt: 32 - Sayı: 4

Analysis of Ki-ras Exon 2 Gene Mutations in 3-Methylcholanthrene and Butylated Hydroxytoluene-Induced Rat Lung Tissues

Fikriye POLAT
Öztürk ÖZDEMİR
Şahende ELAGÖZ
Pages
277–282
Publication Date
2008-04-01
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

No point mutation was observed in the Ki-ras gene exon 2.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:3-Methylcholanthrene (MCA) is a polycyclic aromatic hydrocarbon and potent carcinogenic agent that is often used in experimental cancer studies.
📊
Key Findings & Data:The aim of the present study was to investigate the role of the application of MCA and BHT in the development of lung cancer, and to detect any mutation in the Ki-ras gene exon 2.
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Conclusion & Impact:No point mutation was observed in the Ki-ras gene exon 2.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Ki-ras gene · 3-methylcholanthrene · butylated hydroxytoluene

Abstract

3-Methylcholanthrene (MCA) is a polycyclic aromatic hydrocarbon and potent carcinogenic agent that is often used in experimental cancer studies. Butylated hydroxytoluene (BHT) has been widely used for many years as an antioxidant to preserve and stabilize the freshness, nutritional value, flavor, and color of foods. The aim of the present study was to investigate the role of the application of MCA and BHT in the development of lung cancer, and to detect any mutation in the Ki-ras gene exon 2. Rats were killed by cervical dislocation 26 weeks after the last BHT injection and lung tissues were surgically removed. Lung tissues of the control and experiment rat groups were examined for point mutations in the exon 2 of the Ki-ras by PCR based SSCP analysis. No point mutation was observed in the Ki-ras gene exon 2.

Özet

3-Methylcholanthrene (MCA) is a polycyclic aromatic hydrocarbon and potent carcinogenic agent that is often used in experimental cancer studies. Butylated hydroxytoluene (BHT) has been widely used for many years as an antioxidant to preserve and stabilize the freshness, nutritional value, flavor, and color of foods. The aim of the present study was to investigate the role of the application of MCA and BHT in the development of lung cancer, and to detect any mutation in the Ki-ras gene exon 2. Rats were killed by cervical dislocation 26 weeks after the last BHT injection and lung tissues were surgically removed. Lung tissues of the control and experiment rat groups were examined for point mutations in the exon 2 of the Ki-ras by PCR based SSCP analysis. No point mutation was observed in the Ki-ras gene exon 2.

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Cite This Article

POLAT, F., ÖZDEMİR, Ö., ELAGÖZ, Ş. (2008). Analysis of Ki-ras Exon 2 Gene Mutations in 3-Methylcholanthrene and Butylated Hydroxytoluene-Induced Rat Lung Tissues. Turkish Journal of Biology, Cilt: 32 - Sayı: 4, 277–282. https://ajindex.com/articles/182370

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