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Journals / Turkish Journal of Biology / 2010 / Cilt: 34 - Sayı: 2

Antioxidant and antibacterial activities of Ranunculus marginatus var. trachycarpus and R. sprunerianus

Gülen İrem KAYA
- *
Nehir ÜNVER SOMER
Sibel KONYALIOĞLU
H. Tansel YALÇIN
N. Ülkü KARABAY YAVAŞOĞLU
Buket SARIKAYA
Mustafa Ali ÖNÜR
Pages
139–146
Publication Date
2010-02-01
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The minimal inhibitory concentrations (MICs) of all the extracts of both Ranunculus species were found to be between 128 and 256 µg/mL.

📌 3 Key Takeaways

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Objective & Hypothesis:Hexane, ethyl acetate, methanol, and aqueous extracts of Ranunculus marginatus d'Urv. var. trachycarpus (Fisch. & Mey.) Azn. and R. sprunerianus Boiss. were tested in vitro for their antioxidant and antibacterial activities.
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Key Findings & Data:Antioxidant activity of the extracts was determined by DPPH radical scavenging and Trolox equivalent antioxidant capacity assays.
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Conclusion & Impact:The minimal inhibitory concentrations (MICs) of all the extracts of both Ranunculus species were found to be between 128 and 256 µg/mL.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Key words: Antibacterial activity · antioxidant activity · flavonoid

Abstract

Hexane, ethyl acetate, methanol, and aqueous extracts of Ranunculus marginatus d'Urv. var. trachycarpus (Fisch. & Mey.) Azn. and R. sprunerianus Boiss. were tested in vitro for their antioxidant and antibacterial activities. Antioxidant activity of the extracts was determined by DPPH radical scavenging and Trolox equivalent antioxidant capacity assays. Methanol extracts showed the highest antioxidant activity in both assays. The total phenolics in the extracts were determined colorimetrically by using the Folin-Ciocalteau reagent. The total flavonoid content of the extracts was evaluated by a spectrophotometric method. The results obtained in the antioxidant activity tests were in positive correlation with the total phenolic and flavonoid contents of the extracts. An antibacterial activity analysis was carried out using paper disk diffusion and micro-well dilution techniques. All of the extracts displayed antibacterial activity against the tested bacteria in the disk diffusion method. The minimal inhibitory concentrations (MICs) of all the extracts of both Ranunculus species were found to be between 128 and 256 µg/mL.

Özet

Hexane, ethyl acetate, methanol, and aqueous extracts of Ranunculus marginatus d'Urv. var. trachycarpus (Fisch. & Mey.) Azn. and R. sprunerianus Boiss. were tested in vitro for their antioxidant and antibacterial activities. Antioxidant activity of the extracts was determined by DPPH radical scavenging and Trolox equivalent antioxidant capacity assays. Methanol extracts showed the highest antioxidant activity in both assays. The total phenolics in the extracts were determined colorimetrically by using the Folin-Ciocalteau reagent. The total flavonoid content of the extracts was evaluated by a spectrophotometric method. The results obtained in the antioxidant activity tests were in positive correlation with the total phenolic and flavonoid contents of the extracts. An antibacterial activity analysis was carried out using paper disk diffusion and micro-well dilution techniques. All of the extracts displayed antibacterial activity against the tested bacteria in the disk diffusion method. The minimal inhibitory concentrations (MICs) of all the extracts of both Ranunculus species were found to be between 128 and 256 µg/mL.

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Cite This Article

KAYA, G. İ., *, -., SOMER, N. Ü., KONYALIOĞLU, S., YALÇIN, H. T., YAVAŞOĞLU, N. Ü. K., SARIKAYA, B., ÖNÜR, M. A. (2010). Antioxidant and antibacterial activities of Ranunculus marginatus var. trachycarpus and R. sprunerianus. Turkish Journal of Biology, Cilt: 34 - Sayı: 2, 139–146. https://ajindex.com/articles/182359

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