Journals / Turkish Journal of Biology / 2005 / Cilt: 29 - Sayı: 3

Antimicrobial Effects of Ocimum basilicum (Labiatae) Extract

Pages
155–160
Publication Date
2005-03-01
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The minimal inhibition zones (MIC) of the hexane, methanol, and ethanol extracts ranged from 125 to 250 µl/ml, 62.50 to 500 µl/ml, and 125 to 250 µl/ml, respectively.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Ethanol, methanol, and hexane extracts from Ocimum basilicum Labiatae (sweet basil) were investigated for their invitro antimicrobial properties.
📊
Key Findings & Data:A total of 146 microbial organisms belonging to 55 bacteria, and four fungi, and a yeast species were studied using a disk-diffusion and minimal inhibition concentration (MIC) method.
💡
Conclusion & Impact:The minimal inhibition zones (MIC) of the hexane, methanol, and ethanol extracts ranged from 125 to 250 µl/ml, 62.50 to 500 µl/ml, and 125 to 250 µl/ml, respectively.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Ocimum basilicum Labiatae. · antimicrobial activity · medicinal plant

Abstract

Ethanol, methanol, and hexane extracts from Ocimum basilicum Labiatae (sweet basil) were investigated for their invitro antimicrobial properties. A total of 146 microbial organisms belonging to 55 bacteria, and four fungi, and a yeast species were studied using a disk-diffusion and minimal inhibition concentration (MIC) method. The result showed that none of the three extracts tested have antifungal activities, but anticandidal and antibacterial effects. Both the hexane and methanol extracts, but not the ethanol extracts, inhibited three isolates out of 23 strains of Candida albicans studied. All three extract of O. basilicum were different in terms of their antibacterial activities. The hexane extract showed a stronger and broader spectrum of antibacterial activity, followed by the methanol and ethanol extracts, which inhibited 10, 9 and 6% of the 146 bacterial strains tested, respectively. The minimal inhibition zones (MIC) of the hexane, methanol, and ethanol extracts ranged from 125 to 250 µl/ml, 62.50 to 500 µl/ml, and 125 to 250 µl/ml, respectively.

Özet

Ethanol, methanol, and hexane extracts from Ocimum basilicum Labiatae (sweet basil) were investigated for their invitro antimicrobial properties. A total of 146 microbial organisms belonging to 55 bacteria, and four fungi, and a yeast species were studied using a disk-diffusion and minimal inhibition concentration (MIC) method. The result showed that none of the three extracts tested have antifungal activities, but anticandidal and antibacterial effects. Both the hexane and methanol extracts, but not the ethanol extracts, inhibited three isolates out of 23 strains of Candida albicans studied. All three extract of O. basilicum were different in terms of their antibacterial activities. The hexane extract showed a stronger and broader spectrum of antibacterial activity, followed by the methanol and ethanol extracts, which inhibited 10, 9 and 6% of the 146 bacterial strains tested, respectively. The minimal inhibition zones (MIC) of the hexane, methanol, and ethanol extracts ranged from 125 to 250 µl/ml, 62.50 to 500 µl/ml, and 125 to 250 µl/ml, respectively.

🔖

Cite This Article

ADIGÜZEL, A., GÜLLÜCE, M., ŞENGÜL, M., ÖĞÜTCÜ, H., ŞAHİN, F., KARAMAN, İ. (2005). Antimicrobial Effects of Ocimum basilicum (Labiatae) Extract. Turkish Journal of Biology, Cilt: 29 - Sayı: 3, 155–160. https://ajindex.com/articles/182322

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (19)

  1. Saxena VK, Sharma RN. Antimicrobial activity of the essential oil of Lantana aculeata. Fitoterapia 70: 67-70, 1999
  2. Ahmad I, Beg AZ. Antimicrobial and phtytochemical studies on 45 Indian medicinal plants against multi-drug resistant human pathogens. J Ethnopharmacol 74: 113-123, 2001
  3. World Health Organisation. Summary of WHO guidelines for the assessment of herbal medicines. Herbal Gram 28: 13–14, 1993
  4. Özturk M, Özcelik H. Do¤u Anadolu’nun fiifalı Bitkileri. S‹SKAV vakfı, Semih Ofset Matbaacılık, 1991: pp. 27-28
  5. Tada H, Murakami Y, Omoto T et al. Rosmarinic acid and related phenolics in hairy root cultures of Ocimum basilicum. Phytochemistry 42: 431-434, 1996
  6. Reuveni R, Raviv M, Krasnovsky A et al. Compost induces protection against Fusarium oxysporum in sweet basil. Crop Protection 21: 583-587, 2002 7. Chiej R. Encyclopaedia of Medicinal Plants. MacDonald: ISBN 0- 356-10541-5, 1984
  7. Lust J. The Herb Book. Bantam books: ISBN 0-553-23827-2, 1983
  8. Duke JA, Ayensu ES. Medicinal Plants of China. Reference Publications. Inc.: ISBN 0-917256-20-4, 1985
  9. Chopra RN, Nayar SL and Chopra IC. Glossary of Indian Medicinal Plants ( Including the Supplement). Council of Scientific and Industrial Research. New Delhi, 1986
  10. Martin KW, Ernst E. Herbal medicines for treatment of fungal infections: a systematic review of controlled clinical trials. Mycoses 47: 87-92, 2004
  11. Davis J. Inactivation of antibiotics and the dissemination of resistance genes. Science 264: 375-382, 1994
  12. Loper JE, Henkels MD, Roberts RG et al. Evaluation of streptomycin, oxytetracycline, and copper resistance of Erwinia amylovora isolated from pear orchards in Washington State. Plant Dis 75: 287-290, 1991
  13. Service RF. Antibiotics that resist resistance. Science 270: 724- 727, 1995
  14. Ahmad I, Mehmood Z, Mohammad F. Screening of some Indian medicinal plants for their antimicrobial properties. J Ethnopharmacol 62: 183-193, 1998
  15. Clark AM. Natural products as resource for new drugs. Pharmaceut Res 13: 1133-1141, 1996
  16. Cordell GA. Biodiversity and drug discovery(-)a symbiotic relationship. Phytochemistry 55: 463-480, 2000
  17. Lin J, Opoku AR, Geheeb-Keller M et al. Preliminary screening of some traditional zulu medicinal plants for anti-inflammatory and anti-microbial activities. J Ethnopharmacol 68: 267-274, 1999
  18. Murray PR, Baron EJ, Pfaller MA et al. Manual of Chinical Microbiology 6th Edition. ASM: Washington DC, 1995
  19. Zgoda JR, Porte