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Journals / Turkish Journal of Biology / 2014 / Cilt: 38 - Sayı: 2

Identification and expression profiling of CLCuV-responsive transcripts in upland cotton (Gossypium hirsutum L.)

Beenish AFTAB
Muhammad Naveed SHAHID
Sania RIAZ
Adil JAMAL
Bahaeldeen Babiker MOHAMED
Pages
226–237
Publication Date
2014-05-16
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This gene and other identified DETs could provide a valuable resource for the future improvement of cotton varieties against CLCuV infection.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:CLCuV is one of the most serious causes of cotton damage for which no adequate remedy is available.
📊
Key Findings & Data:Seven transcripts have significant homology with known proteins (ribosomal protein, photosystem protein unit, membrane protein, and a predicted hypothetical protein).
💡
Conclusion & Impact:This gene and other identified DETs could provide a valuable resource for the future improvement of cotton varieties against CLCuV infection.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Key words: Differential display · leaf curl virus · stress tolerance

Abstract

CLCuV is one of the most serious causes of cotton damage for which no adequate remedy is available. The possible solution to this problem may be the transfer of resistant genes into the background of agronomical CLCuV-tolerant varieties. In response to viral infection, several plants may show qualitative and quantitative variations in gene expression. To study gene expression between diseased and tolerant plants, DDRT-PCR was performed. Upregulated gene expression was observed among cDNA transcripts screened through random primers. Seven transcripts have significant homology with known proteins (ribosomal protein, photosystem protein unit, membrane protein, and a predicted hypothetical protein). Quantitative real-time PCR showed varied levels of expression in tolerant as compared to CLCuV-diseased leaf samples. The potential candidate transcript DET1 was generated full length as GhLCVR. The deduced amino acid sequence, with a molecular mass of 16.1 kDa, contains a single open reading frame of 142 amino acids. The predicted amino acid sequence shares 33%-80% identities with Glycine max, Populus trichocarpa, Vitis vinifera, Zea mays, Ricinus communis, Arabidopsis thaliana, Picea sitchensis, Physcomitrella patens, and Staphylococcus aureus in descending order. This gene and other identified DETs could provide a valuable resource for the future improvement of cotton varieties against CLCuV infection.

Özet

CLCuV is one of the most serious causes of cotton damage for which no adequate remedy is available. The possible solution to this problem may be the transfer of resistant genes into the background of agronomical CLCuV-tolerant varieties. In response to viral infection, several plants may show qualitative and quantitative variations in gene expression. To study gene expression between diseased and tolerant plants, DDRT-PCR was performed. Upregulated gene expression was observed among cDNA transcripts screened through random primers. Seven transcripts have significant homology with known proteins (ribosomal protein, photosystem protein unit, membrane protein, and a predicted hypothetical protein). Quantitative real-time PCR showed varied levels of expression in tolerant as compared to CLCuV-diseased leaf samples. The potential candidate transcript DET1 was generated full length as GhLCVR. The deduced amino acid sequence, with a molecular mass of 16.1 kDa, contains a single open reading frame of 142 amino acids. The predicted amino acid sequence shares 33%-80% identities with Glycine max, Populus trichocarpa, Vitis vinifera, Zea mays, Ricinus communis, Arabidopsis thaliana, Picea sitchensis, Physcomitrella patens, and Staphylococcus aureus in descending order. This gene and other identified DETs could provide a valuable resource for the future improvement of cotton varieties against CLCuV infection.

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Cite This Article

AFTAB, B., SHAHID, M. N., RIAZ, S., JAMAL, A., MOHAMED, B. B. (2014). Identification and expression profiling of CLCuV-responsive transcripts in upland cotton (Gossypium hirsutum L.). Turkish Journal of Biology, Cilt: 38 - Sayı: 2, 226–237. https://ajindex.com/articles/182225

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References (16)

  1. Akhtar KP, Haq MA, Hussain M, Khan AI (2002a). Whitefly transmitted Gemini virus and associated disorder in cotton: a review. Pak J Phytopath 14: 140–150.
  2. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990). Basic local alignment search tool. J Mol Biol 215: 403–410.
  3. Amrao L, Akhter S, Tahir MN, Amin I, Briddon RW, Mansoor S (2010a). Cotton leaf curl disease in Sindh Province of Pakistan is associated with recombinant begomovirus components. Virus Res 153: 161–165.
  4. Amrao L, Amin I, Shahid MS, Briddon RW, Mansoor S (2010b). Cotton leaf curl disease in resistant cotton is associated with a single begomovirus that lacks an intact transcriptional activator protein. Virus Res 152: 153–163.
  5. Beck EH, Fettig S, Knake C, Hartig K, Bhattarai T (2007). Specific and unspecific responses of plants to cold and drought stress. J Bioscience 32: 501–510.
  6. Bimboim HC, Doly J (1979). A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7: 1513–1523.
  7. Bivalkar-Mehla S, Vakharia J, Mehla R, Abreha M, Kanwar JR, Tikoo A, Chauhan A (2011). Viral RNA silencing suppressors (RSS): novel strategy of viruses to ablate the host RNA interference (RNAi) defense system. Virus Res 155: 1–9.
  8. Bonas U, Lahaye T (2002). Plant disease resistance triggered by pathogen-derived molecules: refined models of specific recognition. Curr Opin Microbio 15: 44–50.
  9. Brandt P, Unseld M, Eckert-Ossenkopp U, Brennicke A (1993). An rps14 pseudogene is transcribed and edited in Arabidopsis mitochondria. Curr Genet 24: 330–336.
  10. Briddon RW (2003). Cotton leaf curl disease, a multicomponent begomovirus complex. Plant Pathol 4: 427–434.
  11. Briddon RW, Markham PG (2000). Cotton leaf curl virus disease. Virus Res 71: 151–159.
  12. Cherepneva GN, Schmidt KH, Kulaeva ON, Oelmüller R, Kusnetsov VV (2003). Expression of the ribosomal proteins S14, S16, L13a and L30 is regulated by cytokinin and abscisic acid: Implication of the involvement of phytohormones in translational processes. Plant Sci 165: 925–932.
  13. Chitnis PR (1996). Photosystem I. Plant Physiol 111: 661–669.
  14. Cushman JC, Bohnert HJ (2000). Genomic approaches to plant stress tolerance. Curr Opin Plant Biol 3: 117–124. de Wit PJ (1992). Molecular characterization of gene-for-gene systems in plant-fungus interactions and the application of avirulence genes in control of plant pathogens. Annu Rev Phytopathol 30: 391–418.
  15. Debouck C (1995). Differential display or differential dismay? Curr Opin Biotechno l6: 597–599.
  16. Don RH, Cox