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Journals / Turkish Journal of Biology / 2016 / Cilt: 40 - Sayı: 4

Identification, characterization, and expression profiling of salt-stress tolerant proton gradient regulator 5 (PGR5) in Gossypium arboreum

Tayyab HUSNAIN
Bushra RASHID
Muhammad Neveed SHAHID
Baheldeen MOHAMED
Javed Iqbal WATTOO
Muhammad Sarfraz KIANI
Adil JAMAL
Beenish AFTAB
Pages
889–898
Publication Date
2022-11-05
DOI
—
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⚡ TL;DR(Too Long; Didn't Read)

The results showed a 7-fold increased expression of GPGR5 in leaf tissue in salt stress and almost no induction of transcription in root and stem tissues in salt stress.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Salinity is among the significant abiotic stresses adversely affecting plant development and yield for a wide range of crops.
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Key Findings & Data:For quantitative and qualitative expression studies of proton gradient regulator 5 (PGR5) under salt stress, differential display RT-PCR and rapid amplification of cDNA ends (RACE) were performed on salt-stress responsive Gossypium arboreum.
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Conclusion & Impact:The results showed a 7-fold increased expression of GPGR5 in leaf tissue in salt stress and almost no induction of transcription in root and stem tissues in salt stress.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Salinity is among the significant abiotic stresses adversely affecting plant development and yield for a wide range of crops. For quantitative and qualitative expression studies of proton gradient regulator 5 (PGR5) under salt stress, differential display RT-PCR and rapid amplification of cDNA ends (RACE) were performed on salt-stress responsive Gossypium arboreum. Alignment of genomic and cDNA sequences revealed that the GPGR5 gene comprises a single open reading frame of 96 amino acids and contains no introns. Alignment of cotton GPGR5 complete amino acid sequence with PGR5 from other plants revealed the following identities: Gossypium raimondii (97%), Amaranthus hybridus (72%), Vitis vinifera (69%), Medicago truncatula (68%), Cucumis melo (62%), Arabidopsis thaliana (62%), Portulaca oleracea (61%), Portulaca grandiflora (60%), and Zea mays (55%). The expression profile was studied in different plant tissues (stem, leaf, and root) under the abiotic stresses salt, drought, and cold. The results showed a 7-fold increased expression of GPGR5 in leaf tissue in salt stress and almost no induction of transcription in root and stem tissues in salt stress. This gene has a good expression pattern under cold stress compared to salt and drought. In future studies, GPGR5 may be used as a prognostic marker for the development of abiotic-stress-tolerant plants.

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Cite This Article

HUSNAIN, T., RASHID, B., SHAHID, M. N., MOHAMED, B., WATTOO, J. I., KIANI, M. S., JAMAL, A., AFTAB, B. (2022). Identification, characterization, and expression profiling of salt-stress tolerant proton gradient regulator 5 (PGR5) in Gossypium arboreum. Turkish Journal of Biology, Cilt: 40 - Sayı: 4, 889–898. https://ajindex.com/articles/654424

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