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Journals / Turkish Journal of Biology / 2014 / Cilt: 38 - Sayı: 3

Identification of an AFLP marker linked with yellow rust resistance in wheat (Triticum aestivum L.)

Handan BALTA
Özge KARAKAŞ METİN
Funda ŞENTÜRK AKFIRAT
Fahriye ERTUĞRUL
Semra HASANÇEBİ
Pages
371–379
Publication Date
2014-05-16
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Future research will obtain more adjacent sequences associated with the polymorphic M-ACG/P-GAC133bp marker by the PCR walking method to design SCAR markers for wheat breeding programs.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:An important application of molecular markers in plant systems involves improvement in the efficiency of conventional plant breeding by carrying out indirect selection through molecular markers linked to the traits of interest.
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Key Findings & Data:DNA isolated from the selected yellow rust tolerant and susceptible F2 individuals derived from a cross between Izgi2001 (resistant) and ES14 (susceptible) at seedling and adult stage was used for bulked segregant analysis combined with AFLP.
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Conclusion & Impact:Future research will obtain more adjacent sequences associated with the polymorphic M-ACG/P-GAC133bp marker by the PCR walking method to design SCAR markers for wheat breeding programs.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Key words: AFLP · bread wheat · disease resistance

Abstract

An important application of molecular markers in plant systems involves improvement in the efficiency of conventional plant breeding by carrying out indirect selection through molecular markers linked to the traits of interest. AFLP analysis was used to identify molecular markers associated with yellow rust resistance in wheat (Triticum aestivum L.) in this study. DNA isolated from the selected yellow rust tolerant and susceptible F2 individuals derived from a cross between Izgi2001 (resistant) and ES14 (susceptible) at seedling and adult stage was used for bulked segregant analysis combined with AFLP. From the screening of 34 PstI/MseI AFLP primer combinations, the AFLP marker P-GAC/M-ACG (133 bp) was identified and presented in the resistant parent and resistant F2 individuals but not in the susceptible ones. Future research will obtain more adjacent sequences associated with the polymorphic M-ACG/P-GAC133bp marker by the PCR walking method to design SCAR markers for wheat breeding programs.

Özet

An important application of molecular markers in plant systems involves improvement in the efficiency of conventional plant breeding by carrying out indirect selection through molecular markers linked to the traits of interest. AFLP analysis was used to identify molecular markers associated with yellow rust resistance in wheat (Triticum aestivum L.) in this study. DNA isolated from the selected yellow rust tolerant and susceptible F2 individuals derived from a cross between Izgi2001 (resistant) and ES14 (susceptible) at seedling and adult stage was used for bulked segregant analysis combined with AFLP. From the screening of 34 PstI/MseI AFLP primer combinations, the AFLP marker P-GAC/M-ACG (133 bp) was identified and presented in the resistant parent and resistant F2 individuals but not in the susceptible ones. Future research will obtain more adjacent sequences associated with the polymorphic M-ACG/P-GAC133bp marker by the PCR walking method to design SCAR markers for wheat breeding programs.

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Cite This Article

BALTA, H., METİN, Ö. K., AKFIRAT, F. Ş., ERTUĞRUL, F., HASANÇEBİ, S. (2014). Identification of an AFLP marker linked with yellow rust resistance in wheat (Triticum aestivum L.). Turkish Journal of Biology, Cilt: 38 - Sayı: 3, 371–379. https://ajindex.com/articles/181792

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