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Journals / Turkish Journal of Biology / 2013 / Cilt: 37 - Sayı: 3

Evaluation of the genetic relationship between Fritillaria species from Turkey’s flora using fluorescent-based AFLP

Özge Karakaş METİN
Mine TÜRKTAŞ
Meral ASLAY
Erdal KAYA
Pages
273–279
Publication Date
2013-03-01
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This primary report on AFLP analysis of Fritillaria species could facilitate achieving a deeper understanding of genetic relationships between the species.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, the genetic relationships among 12 Fritillaria taxa collected from different regions of Turkey were investigated using the amplified fragment length polymorphism (AFLP) technique.
📊
Key Findings & Data:The analyses generated congruent results with each other and with the previous studies.
💡
Conclusion & Impact:This primary report on AFLP analysis of Fritillaria species could facilitate achieving a deeper understanding of genetic relationships between the species.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Key words: AFLP · Fritillaria · neighbor joining

Abstract

In this study, the genetic relationships among 12 Fritillaria taxa collected from different regions of Turkey were investigated using the amplified fragment length polymorphism (AFLP) technique. Seven primer pair combinations were used for the analysis. The neighbor-joining and principal coordinate analysis methods were implemented to identify the taxonomy of the species. The analyses generated congruent results with each other and with the previous studies. The neighbor-joining analysis clustered the species into 3 groups, while the principal coordinates analysis did not support separation of the third group. The subgenera Fritillaria and Petilium diverged from each other with strong support in all analyses. The neighbor-joining tree further divided the members of subgenus Fritillaria into 2 subgroups. Statistical analysis of the data set using NTSYS 2.1 showed a very good fit between the data matrix and the cophenetic matrix for the AFLP data with an r-value of 0.91. Generating a high polymorphic band profile, this analysis presented the feasibility of using AFLP for a genetic relationship analysis of Fritillaria. This primary report on AFLP analysis of Fritillaria species could facilitate achieving a deeper understanding of genetic relationships between the species.

Özet

In this study, the genetic relationships among 12 Fritillaria taxa collected from different regions of Turkey were investigated using the amplified fragment length polymorphism (AFLP) technique. Seven primer pair combinations were used for the analysis. The neighbor-joining and principal coordinate analysis methods were implemented to identify the taxonomy of the species. The analyses generated congruent results with each other and with the previous studies. The neighbor-joining analysis clustered the species into 3 groups, while the principal coordinates analysis did not support separation of the third group. The subgenera Fritillaria and Petilium diverged from each other with strong support in all analyses. The neighbor-joining tree further divided the members of subgenus Fritillaria into 2 subgroups. Statistical analysis of the data set using NTSYS 2.1 showed a very good fit between the data matrix and the cophenetic matrix for the AFLP data with an r-value of 0.91. Generating a high polymorphic band profile, this analysis presented the feasibility of using AFLP for a genetic relationship analysis of Fritillaria. This primary report on AFLP analysis of Fritillaria species could facilitate achieving a deeper understanding of genetic relationships between the species.

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Cite This Article

METİN, Ö. K., TÜRKTAŞ, M., ASLAY, M., KAYA, E. (2013). Evaluation of the genetic relationship between Fritillaria species from Turkey’s flora using fluorescent-based AFLP. Turkish Journal of Biology, Cilt: 37 - Sayı: 3, 273–279. https://ajindex.com/articles/181655

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References (11)

  1. Kosenko VN. Pollen morphology of genus Fritillaria (Liliaceae). Botanicheskiy Zhurnal (Leningrad) 76: 1201–10, 19 Zhang DQ, Gao LM, Yang YP. Genetic diversity and structure of a traditional Chinese medicinal plant species, Fritillaria cirrhosa (Liliaceae) in southwest China and implications for its conservation. Biochem Syst Ecol 38: 236–42, 2010.
  2. Kaneko K, Tanaka M, Nakaoka U et al. Camtschatcanidine, an alkaloid from Fritillaria camtschatcensis. Phytochemistry 20: 327–9, 1981.
  3. Kitajima J, Noda N, Ida Y et al. Steroidal alkaloids of fresh bulbs of Fritillaria thunbergi and of crude drug “bai-mo” prepared therefrom. Heterocycles 15: 791–6, 1981.
  4. Kaneko K, Katsuhara T, Kitamura Y et al. New steroidal alkaloids from the Chinese herb drug “bei-mu”. Chem Pharm Bull 36: 4700–5, 1988.
  5. Lin G, Li P, Li SL et al. Chromatographic analysis of Fritillaria isosteroidal alkaloids, the active ingredients of Beimu, the antitussive traditional Chinese medicinal herb. J Chromatogr A 935: 321–38, 2001.
  6. Jefferson-Brown M, Pratt K. The Gardener’s Guide to Growing Fritillaries. David & Charles. Devon, England; 1997. Özhatay N. Fritillaria L. In: Güner A, Özhatay N, Ekim T et al. eds. Flora of Turkey and the East Aegean Islands (Suppl. 2). Edinburgh University Press; 2000: pp. 243–6.
  7. Tekşen M, Aytaç Z. New Fritillaria L. taxa from Turkey. Israel J Plant Sci 52: 357–65, 2004.
  8. Özhatay N, Kültür Ş. Check-list of additional taxa to the supplement Flora of Turkey III. Turk J Bot 30: 281–316, 2006.
  9. Özler H, Pehlivan S. Comparison of pollen morphological structures of some taxa belonging to Asparagus L. and Fritillaria L. (Liliaceae) from Turkey. Bangladesh J Botany 36: 111–20, 200 Özhatay N, Kültür Ş, Aslan S. Check-list of additional taxa to the supplement Flora of Turkey IV. Turk J Bot 33: 191 –226, 2009.
  10. Tekşen M, Aytaç Z. The revision of the genus Fritillaria L. (Liliaceae) in the Mediterranean region (Turkey). Turk J Bot 35: 447–78, 2011. Linnaeus C. Species Plantarum. Impensis Laurentii Salvii. Stockholm; 1753: pp. 303–4. Baker JG. Revision of the genera and species of Tulipeae. J Linn Soc. 14: 211–310, 1874. Bentham G, Hooker JD. Fritillaria. Genera Plantarum 3: 817–8, 18 Boissier PE. Fritillaria. Flora Orientalis 5: 176–90, 1882. Turrill WB, Sealy JR. Studies in the genus Fritillaria (Liliaceae). Hooker’s Icon Pl 39: 1–2, 1980. Rix EM. Fritillaria: A Revised Classification. The Fritillaria Group of the Alpine Garden Society. Edinburgh; 2001.
  11. Fay MF, Chase MW. Modern concepts o