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Journals / Turkish Journal of Biology / 2018 / Cilt: 42 - Sayı: 4

The effect of TWIST silencing in metastatic chordoma cells

Esra AYDEMİR
Ezgi KAŞIKCI
Ömer Faruk BAYRAK
Fikrettin ŞAHİN
Pages
279–285
Publication Date
2018-08-09
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This study indicates that Twist might have a role in metastatic chordoma cells.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Chordoma is a slowly growing and invasive bone tumor with a tendency to metastasize locally in advanced stages.
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Key Findings & Data:The TWIST gene was silenced by short hairpins in chordoma cell line MUG-Chor1 and effects on metastasis were investigated by wound healing/gap closure and invasion assays.
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Conclusion & Impact:This study indicates that Twist might have a role in metastatic chordoma cells.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Cancer · epithelial-mesenchymal transition · chordoma

Abstract

Chordoma is a slowly growing and invasive bone tumor with a tendency to metastasize locally in advanced stages. It is essential to discover new therapeutics that target genes involved in the metastasis of chordoma. Epithelial-mesenchymal transition (EMT) might robustly influence the metastasis of a tumor bulk. To our knowledge, this is the first time to show that EMT might have a role in chordoma metastasis. In this study, we aim to investigate the possible role of Twist, a key player transcription factor of EMT, in chordoma metastasis. The TWIST gene was silenced by short hairpins in chordoma cell line MUG-Chor1 and effects on metastasis were investigated by wound healing/gap closure and invasion assays. Twist-silenced MUG-Chor1 cells were found to be less migratory and less invasive when compared to the negative control. This study indicates that Twist might have a role in metastatic chordoma cells.

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Cite This Article

AYDEMİR, E., KAŞIKCI, E., BAYRAK, Ö. F., ŞAHİN, F. (2018). The effect of TWIST silencing in metastatic chordoma cells. Turkish Journal of Biology, Cilt: 42 - Sayı: 4, 279–285. https://ajindex.com/articles/181448

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References (14)

  1. Abrahams A, Parker MI, Prince S (2010). The T-box transcription factor Tbx2: its role in development and possible implication in cancer. IUBMB Life 62: 92-102.
  2. Bates RC, Mercurio AM (2005). The epithelial-mesenchymal transition (EMT) and colorectal cancer progression. Cancer Biol Ther 4: 365-370.
  3. Bergh P, Kindblom LG, Gunterberg B, Remotti F, Ryd W, Meis- Kindblom JM (2000). Prognostic factors in chordoma of the sacrum and mobile spine - A study of 39 patients. Cancer 88: 2122-2134.
  4. Chambers PW, Schwinn CP (1979). Chordoma. A clinicopathologic study of metastasis. Am J Clin Pathol 72: 765-776.
  5. Chen ZF, Behringer RR (1995). Twist is required in head mesenchyme for cranial neural-tube morphogenesis. Gene Dev 9: 686-699.
  6. Cheng GZ, Zhang W, Wang LH (2008). Regulation of cancer cell survival, migration, and invasion by Twist: AKT2 comes to interplay. Cancer Res 68: 957-960.
  7. Croset M, Goehrig D, Frackowiak A, Bonnelye E, Ansieau S, Puisieux A, Clezardin P (2014). TWIST1 expression in breast cancer cells facilitates bone metastasis formation. J Bone Miner Res 29: 1886-1899.
  8. Du WL, Fang Q, Chen Y, Teng JW, Xiao YS, Xie P, Jin B, Wang JQ (2017). Effect of silencing the T-Box transcription factor TBX2 in prostate cancer PC3 and LNCaP cells. Mol Med Rep 16: 6050- 6058. Eccles SA, Welch DR (2007). Metastasis: recent discoveries and novel treatment strategies. Lancet 369: 1742-1757.
  9. Fu J, Qin L, He T, Qin J, Hong J, Wong J, Liao L, Xu J (2011). The TWIST/Mi2/NuRD protein complex and its essential role in cancer metastasis. Cell Res 21: 275-289.
  10. Hong J, Zhou J, Fu J, He T, Qin J, Wang L, Liao L, Xu J (2011). Phosphorylation of serine 68 of Twist1 by MAPKs stabilizes Twist1 protein and promotes breast cancer cell invasiveness. Cancer Res 71: 3980-3990.
  11. Huber MA, Kraut N, Beug H (2005). Molecular requirements for epithelial-mesenchymal transition during tumor progression. Curr Opin Cell Biol 17: 548-558.
  12. Karamchandani J, Wu MY, Das S, Vogel H, Muller P, Cusimano M, Montanera W, Kovacs K (2013). Highly proliferative sellar chordoma with unusually rapid recurrence. Neuropathology 33: 424-430.
  13. Kwok WK, Ling MT, Lee TW, Lau TC, Zhou C, Zhang X, Chua CW, Chan KW, Chan FL, Glackin C et al. (2005). Up-regulation of TWIST in prostate cancer and its implication as a therapeutic target. Cancer Res 65: 5153-5162.
  14. Liu GL, Yang HJ, Liu T, Lin YZ (2014). Expression and significance of E-cadherin, N-cadherin, transforming growth factor-beta 1 and Twist in prostate cancer. Asian Pac J Tr