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Journals / Turkish Journal of Agriculture and Forestry / 2007 / Cilt: 31 - Sayı: 3

Somatic Embryogenesis from Immature Cotyledons of Some European Chestnut (Castanea sativa Mill.) Cultivars

Bekir ŞAN
Mehmet SEZGİN
Hatice DUMANOĞLU
Pages
175–179
Publication Date
2007-03-01
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results showed that the highest percentage of embryogenesis ranged from 40% to 70% and the number of embryos per embryogenic cotyledon varied from 4.04 to 7.70 among the cultivars.

📌 3 Key Takeaways

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Objective & Hypothesis:In 4 European chestnut (Castanea sativa Mill.) cultivars ('Hacıibiş', 'Karamehmet', 'Osmanoğlu' and 'Sarıaşlama'), somatic embryogenesis from immature cotyledons of open-pollinated seeds collected 5, 6, 7, and 8 weeks after anthesis and cultured on Driver and Kuniyuki walnut (DKW) medium was determined in this study.
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Key Findings & Data:The percentage of embryogenic cotyledons and the number of embryos per embryogenic cotyledon were recorded at the end of the second subculture.
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Conclusion & Impact:The results showed that the highest percentage of embryogenesis ranged from 40% to 70% and the number of embryos per embryogenic cotyledon varied from 4.04 to 7.70 among the cultivars.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Chestnut · Castanea sativa Mill. · somatic embryogenesis

Abstract

In 4 European chestnut (Castanea sativa Mill.) cultivars ('Hacıibiş', 'Karamehmet', 'Osmanoğlu' and 'Sarıaşlama'), somatic embryogenesis from immature cotyledons of open-pollinated seeds collected 5, 6, 7, and 8 weeks after anthesis and cultured on Driver and Kuniyuki walnut (DKW) medium was determined in this study. The percentage of embryogenic cotyledons and the number of embryos per embryogenic cotyledon were recorded at the end of the second subculture. The results showed that the highest percentage of embryogenesis ranged from 40% to 70% and the number of embryos per embryogenic cotyledon varied from 4.04 to 7.70 among the cultivars. Cotyledons collected 5, 6, and 7 weeks after anthesis were the most embryogenic.

Özet

In 4 European chestnut (Castanea sativa Mill.) cultivars ('Hacıibiş', 'Karamehmet', 'Osmanoğlu' and 'Sarıaşlama'), somatic embryogenesis from immature cotyledons of open-pollinated seeds collected 5, 6, 7, and 8 weeks after anthesis and cultured on Driver and Kuniyuki walnut (DKW) medium was determined in this study. The percentage of embryogenic cotyledons and the number of embryos per embryogenic cotyledon were recorded at the end of the second subculture. The results showed that the highest percentage of embryogenesis ranged from 40% to 70% and the number of embryos per embryogenic cotyledon varied from 4.04 to 7.70 among the cultivars. Cotyledons collected 5, 6, and 7 weeks after anthesis were the most embryogenic.

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Cite This Article

ŞAN, B., SEZGİN, M., DUMANOĞLU, H. (2007). Somatic Embryogenesis from Immature Cotyledons of Some European Chestnut (Castanea sativa Mill.) Cultivars. Turkish Journal of Agriculture and Forestry, Cilt: 31 - Sayı: 3, 175–179. https://ajindex.com/articles/180383

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