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Journals / Niğde Ömer Halisdemir Üniversitesi Mühendislik Bilimleri Dergisi / 2026 / Cilt: 16

Optimization of bioactive compounds from chicory roots by ultrasound-assisted extraction

Ultrason destekli ekstraksiyon ile hindiba köklerinden biyoaktif bileşiklerin optimizasyonu

Didem Kalkan(NİĞDE ÖMER HALİSDEMİR ÜNİVERSİTESİ)0009-0004-4560-7452
Mehmet Yetişen(NİĞDE ÖMER HALİSDEMİR ÜNİVERSİTESİ)0000-0001-8347-4081
Gözde Doğanay(NİĞDE ÖMER HALİSDEMİR ÜNİVERSİTESİ)0000-0002-1227-9892
Cem Baltacıoğlu(NİĞDE ÖMER HALİSDEMİR ÜNİVERSİTESİ)0000-0001-8308-5991
Hande Baltacıoğlu(NİĞDE ÖMER HALİSDEMİR ÜNİVERSİTESİ)0000-0003-0774-0872
Hasan Tangüler(NİĞDE ÖMER HALİSDEMİR ÜNİVERSİTESİ)0000-0001-6425-9896
Pages
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Publication Date
2026-04-14
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

UAE proved to be a rapid and efficient method for enhancing phenolic recovery.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Chicory roots (Cichorium intybus L.) are rich in phenolic compounds with strong antioxidant activity, making them valuable for functional food and nutraceutical applications.
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Key Findings & Data:This study optimized ultrasound-assisted extraction (UAE) of bioactive compounds from dried chicory roots using an acidified hydroethanolic solvent system (80% ethanol containing 1% HCl, v/v) and a Box–Behnken design.
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Conclusion & Impact:UAE proved to be a rapid and efficient method for enhancing phenolic recovery.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Hindiba kökü · Ultrason destekli ekstraksiyon · Proses optimizasyonu

Abstract

Chicory roots (Cichorium intybus L.) are rich in phenolic compounds with strong antioxidant activity, making them valuable for functional food and nutraceutical applications. This study optimized ultrasound-assisted extraction (UAE) of bioactive compounds from dried chicory roots using an acidified hydroethanolic solvent system (80% ethanol containing 1% HCl, v/v) and a Box–Behnken design. Ultrasound amplitude (60–80%), extraction time (3–7 min), and solid-to-liquid ratio (5–15%) were evaluated for their effects on total phenolic content (TPC) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. ANOVA indicated that all linear factors significantly affected antioxidant activity (p ≤ 0.05), with solid-to-liquid ratio contributing 71.58% of total variability. For TPC, amplitude and time were significant, while concentration showed a marginal effect. Interaction effects, particularly between amplitude and concentration, were notable. Optimal conditions were 74.85% amplitude, 15% concentration, and 3 min, yielding 133.79 ± 4.43 mg GAE/100 g TPC and 61.06 ± 3.36% DPPH inhibition. UAE proved to be a rapid and efficient method for enhancing phenolic recovery.

Özet

Hindiba kökü (Cichorium intybus L.), güçlü antioksidan aktivite gösteren fenolik bileşikler açısından zengindir ve fonksiyonel gıda ile nutrasötik uygulamalar için önemli bir hammaddedir. Bu çalışmada, asitlendirilmiş hidroetanolik çözücü sistemi (hacimce %80 etanol ve %1 HCl) ve Box–Behnken tasarımı kullanılarak kurutulmuş hindiba köklerinden biyoaktif bileşiklerin ultrason destekli ekstraksiyonu (UAE) optimize edildi. Ultrases gücü (%60–80), ekstraksiyon süresi (3–7 dk) ve katı:sıvı oranının (%5–15) toplam fenolik madde (TFM) ve 1,1-diphenyl-2-picrylhydrazyl (DPPH) radikal giderme aktivitesi üzerindeki etkileri incelenmiştir. ANOVA sonuçlarına göre tüm lineer faktörler antioksidan aktiviteyi anlamlı düzeyde etkilemiş (p ≤ 0.05) ve en baskın parametre katı:sıvı oranı olmuştur (%71.58). TFM için güç ve süre anlamlı bulunurken, konsantrasyon sınırda etki göstermiştir. Özellikle güç–konsantrasyon etkileşimi önemli bulunmuştur. Optimum koşullar %74.85 güç, %15 katı:sıvı oranı ve 3 dk olarak belirlenmiş; bu şartlarda 133.79 ± 4.43 mg GAE/100 g TFM ve %61.06 ± 3.36 DPPH inhibisyonu elde edilmiştir. UAE hızlı ve etkili bir ekstraksiyon yöntemidir.

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Cite This Article

Kalkan, D., Yetişen, M., Doğanay, G., Baltacıoğlu, C., Baltacıoğlu, H., Tangüler, H. (2026). Optimization of bioactive compounds from chicory roots by ultrasound-assisted extraction. Niğde Ömer Halisdemir Üniversitesi Mühendislik Bilimleri Dergisi, Cilt: 16. https://doi.org/10.28948/ngumuh.1901134

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