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Journals / Atlas Journal of Medicine / 2026 / Cilt: 6 Sayı: 2

Effect of Hypericum Perforatum Extract on cell viability and p53 localization in HepG2 cells

Hypericum Perforatum Ekstraktının HepG2 hücrelerinde hücre canlılığı ve p53 lokalizasyonu üzerine etkisi

Damla Gökçeoğlu Kayalı(ISTANBUL ATLAS UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES (MEDICINE), DEPARTMENT OF HISTOLOGY AND EMBRYOLOGY (MEDICINE))0000-0003-0674-090X
Rana Karaman(ISTANBUL ATLAS UNIVERSITY, SCHOOL OF MEDICINE)0009-0003-5842-6143
Sıla Keçebaş(ISTANBUL ATLAS UNIVERSITY)0009-0007-0095-7864
Zeynep Akbulut(MALTEPE UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF MEDICAL BIOLOGY AND GENETICS)0000-0002-7526-8496
Pages
86–91
Publication Date
2026-06-30
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: Hypericum perforatum extract may activate p53 pathways, leading to DNA damage and cellular stress responses, suggesting its potential as an anticancer agent worthy of further investigation.

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: This study aims to investigate the potential anticancer effects of Hypericum perforatum on hepatocellular carcinoma cells, with a focus on the role of the p53 protein.
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Key Findings & Data:Materials and Methods: HepG2 cells cultured in DMEM were subjected to daily live-cell imaging under a phase-contrast microscope to monitor cell viability and morphology.
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Conclusion & Impact:Conclusion: Hypericum perforatum extract may activate p53 pathways, leading to DNA damage and cellular stress responses, suggesting its potential as an anticancer agent worthy of further investigation.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:HepG2 · Hypericum Perforatum · p53 Proteini

Abstract

Aim: This study aims to investigate the potential anticancer effects of Hypericum perforatum on hepatocellular carcinoma cells, with a focus on the role of the p53 protein. Materials and Methods: HepG2 cells cultured in DMEM were subjected to daily live-cell imaging under a phase-contrast microscope to monitor cell viability and morphology. Treatment groups received 0.8% Hypericum perforatum extract for 24 hours, supplemented with complete medium. Proliferation was assessed using the CCK-8 (WST-8) assay, and the resulting samples were analyzed spectrophotometrically using a microplate reader (BioTek Synergy H1) at the specified wavelength, with optical density changes reflecting cell viability. The expression of p53 proteins in HepG2 cells was evaluated through immunohistochemical staining. Cells were fixed with appropriate fixatives during both weak adhesion and adhesion phases and then treated with specific primary and secondary antibodies. Data analysis was performed using GraphPad Prism 10.0. Results: Treatment with 0.8% Hypericum perforatum extract significantly reduced cell viability in HepG2 cells, indicating a cytotoxic effect. Additionally, p53 protein was observed to translocate from the cytoplasm to the nucleus in these cells. Conclusion: Hypericum perforatum extract may activate p53 pathways, leading to DNA damage and cellular stress responses, suggesting its potential as an anticancer agent worthy of further investigation.

Özet

Amaç: Bu çalışmanın amacı, p53 proteininin rolüne odaklanarak, Hypericum perforatum'un hepatosellüler karsinoma hücreleri üzerindeki potansiyel antikanser etkilerini araştırmaktır. Malzemeler ve Yöntemler: DMEM'de kültürlenen HepG2 hücreleri, hücre canlılığını ve morfolojisini izlemek için faz kontrast mikroskobu altında günlük canlı hücre görüntülemesine tabi tutuldu. Tedavi gruplarına 24 saat boyunca tam ortamla desteklenmiş %0,8 Hypericum perforatum özütü verildi. Proliferasyon, CCK-8 (WST-8) testi kullanılarak değerlendirildi ve elde edilen örnekler, optik yoğunluk değişimlerinin hücre canlılığını yansıttığı belirtilen dalga boyunda bir mikro plaka okuyucu (BioTek Synergy H1) kullanılarak spektrofotometrik olarak analiz edildi. HepG2 hücrelerindeki p53 proteinlerinin ekspresyonu, immünohistokimyasal boyama yoluyla değerlendirildi. Hücreler, hem zayıf yapışma hem de yapışma fazlarında uygun fiksatiflerle sabitlendi ve ardından spesifik birincil ve ikincil antikorlarla tedavi edildi. Veri analizi GraphPad Prism 10.0 kullanılarak gerçekleştirildi. Sonuçlar: %0,8 Hypericum perforatum özütü ile tedavi, HepG2 hücrelerinde hücre canlılığını önemli ölçüde azalttı ve bu da sitotoksik bir etki olduğunu gösterdi. Ek olarak, p53 proteininin bu hücrelerde sitoplazmadan çekirdeğe taşındığı gözlemlendi. Sonuç: Hypericum perforatum özütü, p53 yollarını aktive ederek DNA hasarına ve hücresel stres tepkilerine yol açabilir ve bu da daha fazla araştırmaya değer bir antikanser ajanı olma potansiyelini göstermektedir.

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Cite This Article

Kayalı, D. G., Karaman, R., Keçebaş, S., Akbulut, Z. (2026). Effect of Hypericum Perforatum Extract on cell viability and p53 localization in HepG2 cells. Atlas Journal of Medicine, Cilt: 6 Sayı: 2, 86–91. https://doi.org/10.54270/atljm.2026.129

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