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Journals / Uşak Üniversitesi Fen ve Doğa Bilimleri Dergisi / 2021 / Cilt: 5 Sayı: 2

DETERMINATION OF GENOTYPIC PROFILES OF ESCHERICIA COLI STRAINS BY PFGE MOLECULAR METHOD

ESCHERICHIA COLI SUŞLARININ GENOTİP PROFİLLERİNİN PFGE MOLEKÜLER YÖNTEMİ İLE BELİRLENMESİ

Muhammed İbrahim Yavan(UŞAK ÜNİVERSİTESİ, FEN BİLİMLERİ ENSTİTÜSÜ)0000-0002-8089-2373
Alper Karagöz(UŞAK ÜNİVERSİTESİ, FEN BİLİMLERİ ENSTİTÜSÜ)0000-0002-8178-223X
Pages
157–164
Publication Date
2021-12-29
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Coli strains selected in the study were typified by Pulse field gel electrophoresis.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:From the family Enterobacteriaceae is Escherichia coli ( E.
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Key Findings & Data:It is of great importance to reduce cross-contamination from public areas and to examine and reveal the genotype structure of the bacteria in question, i.e. to reach fingerprints, subtype sequences of strains. 50 E.
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Conclusion & Impact:Coli strains selected in the study were typified by Pulse field gel electrophoresis.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Alt Tiplendirme · Escherichia Coli · Genotiplendirme

Abstract

From the family Enterobacteriaceae is Escherichia coli ( E. Coli) is a pathogenic bacterium that is becoming increasingly important, especially in hospitals, common social areas. Thanks to the broad-spectrum beta-lactams (ESBL) they produce, they are usually multi-resistant and are common in intestinal and urinary tract infections. It is of great importance to reduce cross-contamination from public areas and to examine and reveal the genotype structure of the bacteria in question, i.e. to reach fingerprints, subtype sequences of strains. 50 E. Coli strains selected in the study were typified by Pulse field gel electrophoresis.

Özet

From the family Enterobacteriaceae is Escherichia coli ( E. Coli) is a pathogenic bacterium that is becoming increasingly important, especially in hospitals, common social areas. Thanks to the broad-spectrum beta-lactams (ESBL) they produce, they are usually multi-resistant and are common in intestinal and urinary tract infections. It is of great importance to reduce cross-contamination from public areas and to examine and reveal the genotype structure of the bacteria in question, i.e. to reach fingerprints, subtype sequences of strains. 50 E. Coli strains selected in the study were typified by Pulse field gel electrophoresis.

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Cite This Article

Yavan, M. İ., Karagöz, A. (2021). DETERMINATION OF GENOTYPIC PROFILES OF ESCHERICIA COLI STRAINS BY PFGE MOLECULAR METHOD. Uşak Üniversitesi Fen ve Doğa Bilimleri Dergisi, Cilt: 5 Sayı: 2, 157–164. https://doi.org/10.47137/usufedbid.956913

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References (3)

  1. Referans 1: Baştürk, S., Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa ve Acinetobacter baumannii Suşlarında Çeşitli Kinolon 49 Grubu Antibiyotiklerin 63 Duyarlılıklarının Araştırılması, Uzmanlık Tezi, Sağlık Bakanlığı Haseki Eğitim ve Araştırma Hastanesi, İstanbul, 2005.
  2. Referans 2: Nameghi., S. A., Genotyping Escherichia coli isolates by Pulsed-Field Gel Electrophoresis, Södertörn Högskola Stockholm,Yüksek lisans tezi. New England BıoLabs, 2007.
  3. Refereans 3: Félix, B., Dao, T. T., Lombard, B., Brisabois, A. A. A. and Roussel, S., The Use of Pulsed Field Gel Electrophoresis in Listeria monocytogenes Sub-Typing Harmonization at the European Union Level, Gel Electrophoresis –Principles and Basics (Edited by Magdeldin, S.,), 2012.