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Journals / Uşak Üniversitesi Fen ve Doğa Bilimleri Dergisi / 2026 / Cilt: 10 Sayı: 1

Safety, Stability, and Industrial Applications of Probiotic Bacillus Strains

Probiyotik Bacillus Suşlarının Güvenliği, Dayanıklılığı ve Endüstriyel Uygulamaları

Tesnim Ataki(Gedik Piliç Ar-Ge Merkezi)0009-0002-9592-1496
Abdullah Dikici(UŞAK ÜNİVERSİTESİ)0000-0001-7302-8766
Pages
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Publication Date
2026-10-12
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, the use of spore-forming probiotics, supported by science-based safety assessments and molecular taxonomic classification, offers considerable commercial and biotechnological potential for the development of innovative functional foods with enhanced processing resilience and shelf-life stability.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The growing health awareness among consumers has rapidly increased interest in functional foods within the food industry.
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Key Findings & Data:The limited ability of conventional probiotic microorganisms to maintain viability under high temperatures, pressure, dehydration, and the acidic conditions of the gastrointestinal tract has accelerated the search for alternative probiotic sources.
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Conclusion & Impact:In conclusion, the use of spore-forming probiotics, supported by science-based safety assessments and molecular taxonomic classification, offers considerable commercial and biotechnological potential for the development of innovative functional foods with enhanced processing resilience and shelf-life stability.
🔬Design:Review & Systematic Survey
📍Scope:Comprehensive Sample
⚙️Variables:Bacillus spp. · endospor · proses dayanıklılığı

Abstract

The growing health awareness among consumers has rapidly increased interest in functional foods within the food industry. The limited ability of conventional probiotic microorganisms to maintain viability under high temperatures, pressure, dehydration, and the acidic conditions of the gastrointestinal tract has accelerated the search for alternative probiotic sources. In this context, spore-forming Bacillus species have emerged as strategic ingredients in functional food development owing to the exceptional resistance conferred by their endospore structures. This review comprehensively evaluates the endospore structure, cellular mechanisms, processing tolerance, strain-specific safety profiles, and applications in modern food matrices of Bacillus coagulans, Bacillus subtilis, and Bacillus clausii, which have gained considerable importance in both food and clinical applications. Current evidence indicates that, due to their robust endospore architecture, these strains can maintain stability without requiring additional microencapsulation techniques or cold-chain logistics in most applications while preserving their biological efficacy across a wide range of food products, from bakery products to fermented beverages. Nevertheless, given the heterogeneous nature of the genus Bacillus, phenotypic and genotypic safety assessments, the exclusion of virulence genes, evaluation of biogenic amine production potential, and screening for acquired antibiotic resistance profiles associated with the risk of horizontal gene transfer are critical considerations in industrial strain selection. In conclusion, the use of spore-forming probiotics, supported by science-based safety assessments and molecular taxonomic classification, offers considerable commercial and biotechnological potential for the development of innovative functional foods with enhanced processing resilience and shelf-life stability.

Özet

Tüketicilerin sağlık bilincindeki artış, gıda endüstrisinde fonksiyonel ürünlere olan ilgiyi hızla artırmıştır. Geleneksel probiyotik mikroorganizmaların yüksek sıcaklık, basınç, dehidrasyon ve gastrointestinal sistemin asidik koşulları karşısında canlılıklarını sınırlı düzeyde koruyabilmesi, alternatif probiyotik kaynaklara yönelimi hızlandırmıştır. Bu bağlamda spor oluşturan Bacillus türleri, endospor yapılarının sağladığı yüksek direnç nedeniyle fonksiyonel gıda geliştirmede stratejik bir bileşen olarak öne çıkmaktadır. Bu derlemede, gıda ve klinik uygulamalarda önemli yere sahip Bacillus coagulans, Bacillus subtilis ve Bacillus clausii suşlarının spor yapısı, hücresel mekanizmaları, proses dayanıklılığı, suş-spesifik güvenlik profilleri ve modern gıda matrislerindeki uygulamaları bütüncül bir yaklaşımla değerlendirilmiştir. Mevcut bulgular, bu suşların koruyucu spor mimarileri sayesinde mikroenkapsülasyon tekniklerine veya soğuk zincir lojistiğine çoğu durumda ek bir gereksinim duymadan kararlılık gösterebildiğini ve fırıncılık ürünlerinden fermente içeceklere kadar geniş bir gıda yelpazesinde biyolojik etkinliklerini sürdürebildiğini ortaya koymaktadır. Bununla birlikte, Bacillus cinsinin heterojen yapısı nedeniyle endüstriyel suş seçiminde fenotipik ve genotipik güvenlik kriterleri, virülans genlerinin dışlanması, biyojenik amin potansiyeli ve yatay gen transferi riski taşıyan kazanılmış antibiyotik direnç profilleri kritik önem taşımaktadır. Sonuç olarak, bilimsel temelli güvenlik analizleri ve moleküler taksonomik sınıflandırmalarla desteklenen sporlu probiyotik kullanımı, yüksek işleme ve raf ömrü stabilitesine sahip yenilikçi fonksiyonel gıdaların geliştirilmesinde yüksek ticari ve biyoteknolojik potansiyel sunmaktadır.

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Cite This Article

Ataki, T., Dikici, A. (2026). Safety, Stability, and Industrial Applications of Probiotic Bacillus Strains. Uşak Üniversitesi Fen ve Doğa Bilimleri Dergisi, Cilt: 10 Sayı: 1. https://doi.org/10.47137/usufedbid.1988852

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References (93)

  1. Konuray G, Erginkaya Z. Potential Use of Bacillus Coagulans in the Food Industry. Foods. 13 Haziran 2018;7(6):92. doi:10.3390/foods7060092
  2. Awulachew MT. Probiotics and thermal processing: A review of challenges and protective strategies. Lett Food Res. 06 Eylül 2025;1(4). doi:10.26656/lifr.1.e25057
  3. Payne J, Bellmer D, Jadeja R, Muriana P. The Potential of Bacillus Species as Probiotics in the Food Industry: A Review. Foods. 02 Ağustos 2024;13(15):2444. doi:10.3390/foods13152444
  4. Belkaid Y, Hand TW. Role of the Microbiota in Immunity and Inflammation. Cell. Mart 2014;157(1):121-41. doi:10.1016/j.cell.2014.03.011
  5. FAO/WHO. Guidelines for the Evaluation of Probiotics in Food. 2002
  6. Chugh B, Kamal-Eldin A. Bioactive compounds produced by probiotics in food products. Curr Opin Food Sci. Nisan 2020;32:76-82. doi:10.1016/j.cofs.2020.02.003
  7. Hill C, Guarner F, Reid G, Gibson GR, Merenstein DJ, Pot B, vd. The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nat Rev Gastroenterol Hepatol. Ağustos 2014;11(8):506-14. doi:10.1038/nrgastro.2014.66
  8. Elshaghabee FMF, Rokana N, Gulhane RD, Sharma C, Panwar H. Bacillus As Potential Probiotics: Status, Concerns, and Future Perspectives. Front Microbiol. 10 Ağustos 2017;8:1490. doi:10.3389/fmicb.2017.01490
  9. Madoramae H, Bhoopong P, Lertcanawanichakul M. Spore-forming lactic acid bacteria: Stability, health benefits, and applications. Food Humanity. Aralık 2025;5:100946. doi:10.1016/j.foohum.2025.100946
  10. Mani-López E, Ramírez-Corona N, López-Malo A. Advances in probiotic incorporation into cereal-based baked foods: Strategies, viability, and effects–A review. Appl Food Res. Aralık 2023;3(2):100330. doi:10.1016/j.afres.2023.100330
  11. Burgain J, Gaiani C, Linder M, Scher J. Encapsulation of probiotic living cells: From laboratory scale to industrial applications. J Food Eng. Haziran 2011;104(4):467-83. doi:10.1016/j.jfoodeng.2010.12.031
  12. Nicolas GM. Secondary Metabolites from Bacillus spp. probiotics as potential treatments for multidrug-resistant pathogens: A comprehensive review. Curr Res Microb Sci. 2025;8:100392. doi:10.1016/j.crmicr.2025.100392
  13. Luo H, Yang R, Zhao Y, Wang Z, Liu Z, Huang M, vd. Recent advances and strategies in process and strain engineering for the production of butyric acid by microbial fermentation. Bioresour Technol. Nisan 2018;253:343-54. doi:10.1016/j.biortech.2018.01.007
  14. Miao R, Zhu X, Wan C, Wang Z, Wen Y, Li Y. Effect of Clostridium�butyricum supplementation on the development of intestinal flora and the immune system of neonatal mice. Exp Ther Med. 07 Kasım 2017. doi:10.3892/etm.2017.5461
  15. Guo P, Zhang K, Ma X, He P. Clostridium species as probiotics: potentials and challenges. J Anim Sci Biotechnol. Aralık 2020;11(1):24. doi:10.1186/s40104-019-0402-1
  16. Grenda T, Grenda A, Domaradzki P, Krawczyk P, Kwiatek K. Probiotic Potential of Clostridium spp.—Advantages and Doubts. Curr Issues Mol Biol. 07 Temmuz 2022;44(7):3118-30. doi:10.3390/cimb44070215
  17. Paredes-Sabja D, Shen A, Sorg JA. Clostridium difficile spore biology: sporulation, germination, and spore structural proteins. Trends Microbiol. Temmuz 2014;22(7):406-16. doi:10.1016/j.tim.2014.04.003
  18. Cutting SM. Bacillus probiotics. Food Microbiol. Nisan 2011;28(2):214-20. doi:10.1016/j.fm.2010.03.007
  19. Setlow P. Spore Resistance Properties. Eichenberger P, Driks A, editörler. Microbiol Spectr. 19 Eylül 2014;2(5):2.5.11. doi:10.1128/microbiolspec.TBS-0003-2012
  20. Foligné B, Daniel C, Pot B. Probiotics from research to market: the possibilities, risks and challenges. Curr Opin Microbiol. Haziran 2013;16(3):284-92. doi:10.1016/j.mib.2013.06.008
  21. Raman J, Noh JS, Kim JS, Cho G, Kim DH, Song J, vd. Role of Bacillus Species in Food Industry: Advantages and Limitations. J Microbiol Biotechnol. 22 Ekim 2025;35:e2507043. doi:10.4014/jmb.2507.07043
  22. Leñini C, Rodriguez Ayala F, Goñi AJ, Rateni L, Nakamura A, Grau RR. Probiotic properties of Bacillus subtilis DG101 isolated from the traditional Japanese fermented food nattō. Front Microbiol. 28 Eylül 2023;14:1253480. doi:10.3389/fmicb.2023.1253480
  23. De Castro JA, Kesavelu D, Lahiri KR, Chaijitraruch N, Chongsrisawat V, Jog PP, vd. Recommendations for the adjuvant use of the poly-antibiotic–resistant probiotic Bacillus clausii (O/C, SIN, N/R, T) in acute, chronic, and antibiotic-associated diarrhea in children: consensus from Asian experts. Trop Dis Travel Med Vaccines. Aralık 2020;6(1):21. doi:10.1186/s40794-020-00120-4
  24. De Castro JAA, Guno MJVR, Perez MO. Bacillus clausii as adjunctive treatment for acute community-acquired diarrhea among Filipino children: a large-scale, multicenter, open-label study (CODDLE). Trop Dis Travel Med Vaccines. Aralık 2019;5(1):14. doi:10.1186/s40794-019-0089-5
  25. Almada-Érix CN, Almada CN, Souza Pedrosa GT, Paulo Biachi J, Bonatto MS, Schmiele M, vd. Bread as probiotic carriers: Resistance of Bacillus coagulans GBI-30 6086 spores through processing steps. Food Res Int. Mayıs 2022;155:111040. doi:10.1016/j.foodres.2022.111040
  26. Soares MB, Martinez RCR, Pereira EPR, Balthazar CF, Cruz AG, Ranadheera CS, vd. The resistance of Bacillus, Bifidobacterium, and Lactobacillus strains with claimed probiotic properties in different food matrices exposed to simulated gastrointestinal tract conditions. Food Res Int. Kasım 2019;125:108542. doi:10.1016/j.foodres.2019.108542
  27. Boone DR, Castenholz RW, Garrity GM, editörler. Bergey’s manual of systematic bacteriology. 2. bs. C. 1. New York: Springer; 2001
  28. Bernardeau M, Lehtinen MJ, Forssten SD, Nurminen P. Importance of the gastrointestinal life cycle of Bacillus for probiotic functionality. J Food Sci Technol. Temmuz 2017;54(8):2570-84. doi:10.1007/s13197-017-2688-3
  29. Davis C. Enumeration of probiotic strains: Review of culture-dependent and alternative techniques to quantify viable bacteria. J Microbiol Methods. Ağustos 2014;103:9-17. doi:10.1016/j.mimet.2014.04.012
  30. Soares MB, Almada CN, Pereira EPR, Ferreira BM, Balthazar CF, Khorshidian N, vd. Review - Sporeforming probiotic bacteria: Characteristics, health benefits, and technological aspects for their applications in foods and beverages. Trends Food Sci Technol. Ağustos 2023;138:453-69. doi:10.1016/j.tifs.2023.06.029
  31. Driks A. Overview: development in bacteria: spore formation in Bacillus subtilis. Cell Mol Life Sci CMLS. 01 Mart 2002;59(3):389-91. doi:10.1007/s00018-002-8430-x
  32. Riesenman PJ, Nicholson WL. Role of the Spore Coat Layers in Bacillus subtilis Spore Resistance to Hydrogen Peroxide, Artificial UV-C, UV-B, and Solar UV Radiation. Appl Environ Microbiol. Şubat 2000;66(2):620-6. doi:10.1128/AEM.66.2.620-626.2000
  33. Setlow P. Spores of Bacillus subtilis: their resistance to and killing by radiation, heat and chemicals. J Appl Microbiol. Eylül 2006;101(3):514-25. doi:10.1111/j.1365-2672.2005.02736.x
  34. Cenci G, Trotta F, Caldini G. Tolerance to challenges miming gastrointestinal transit by spores and vegetative cells of Bacillus clausii. J Appl Microbiol. Aralık 2006;101(6):1208-15. doi:10.1111/j.1365-2672.2006.03042.x
  35. Khatri I, Sharma G, Subramanian S. Composite genome sequence of Bacillus clausii, a probiotic commercially available as Enterogermina®, and insights into its probiotic properties. BMC Microbiol. Aralık 2019;19(1):307. doi:10.1186/s12866-019-1680-7
  36. Mazzantini D, Calvigioni M, Celandroni F, Saba A, Ghelardi E. In Vitro Analysis of an Alkalihalobacillus clausii Spore-Based Probiotic Formulation Clarifies the Mechanisms Underlying Its Beneficial Properties. Biomolecules. 08 Eylül 2025;15(9):1294. doi:10.3390/biom15091294
  37. Lu S, Liao X, Zhang L, Fang Y, Xiang M, Guo X. Nutrient L-Alanine-Induced Germination of Bacillus Improves Proliferation of Spores and Exerts Probiotic Effects in vitro and in vivo. Front Microbiol. 02 Aralık 2021;12:796158. doi:10.3389/fmicb.2021.796158
  38. Paidhungat M, Ragkousi K, Setlow P. Genetic Requirements for Induction of Germination of Spores of Bacillus subtilis by Ca2+ -Dipicolinate. J Bacteriol. 15 Ağustos 2001;183(16):4886-93. doi:10.1128/JB.183.16.4886-4893.2001
  39. Abriouel H, Franz CMAP, Omar NB, Gálvez A. Diversity and applications of Bacillus bacteriocins. FEMS Microbiol Rev. Ocak 2011;35(1):201-32. doi:10.1111/j.1574-6976.2010.00244.x
  40. Cutting SM, Ricca E. Bacterial spore-formers: friends and foes. FEMS Microbiol Lett. Eylül 2014;358(2):107-9. doi:10.1111/1574-6968.12572
  41. Rehamnia B, Lee NM, Kuktaite R, Kacem Chaouche N. Screening of Spore-Forming Bacteria with Probiotic Potential in Pristine Algerian Caves. Tang X, editör. Microbiol Spectr. 26 Ekim 2022;10(5):e00248-22. doi:10.1128/spectrum.00248-22
  42. Adibpour N, Hosseininezhad M, Pahlevanlo A. Application of spore-forming probiotic Bacillus in the production of Nabat - A new functional sweetener. LWT. Ekim 2019;113:108277. doi:10.1016/j.lwt.2019.108277
  43. Shaikh SS, Joshi C, Malek F, Malik A, Gandhi M. Food Storage, Processing and Genetic Stability Studies of Bacillus (Heyndrickxia) coagulans BCP92 (MTCC 25460). Appl Food Biotechnol. 24 Haziran 2024;11(1):e22. doi:10.22037/afb.v11i1.44919
  44. Payne J, Bellmer D, Jadeja R. Determining the viability and stability of Bacillus in baked products. LWT. Şubat 2025;218:117519. doi:10.1016/j.lwt.2025.117519
  45. Spencer RC. Bacillus anthracis. J Clin Pathol. Mart 2003;56(3):182-7. doi:10.1136/jcp.56.3.182
  46. Todorov SD, Ivanova IV, Popov I, Weeks R, Chikindas ML. Bacillus spore-forming probiotics: benefits with concerns? Crit Rev Microbiol. 04 Temmuz 2022;48(4):513-30. doi:10.1080/1040841X.2021.1983517
  47. Lee NK, Kim WS, Paik HD. Bacillus strains as human probiotics: characterization, safety, microbiome, and probiotic carrier. Food Sci Biotechnol. Ekim 2019;28(5):1297-305. doi:10.1007/s10068-019-00691-9
  48. Stenfors Arnesen LP, Fagerlund A, Granum PE. From soil to gut: Bacillus cereus and its food poisoning toxins. FEMS Microbiol Rev. Temmuz 2008;32(4):579-606. doi:10.1111/j.1574-6976.2008.00112.x
  49. Jeżewska-Frąckowiak J, Seroczyńska K, Banaszczyk J, Woźniak D, Żylicz-Stachula A, Skowron PM. The promises and risks of probiotic Bacillus species. Acta Biochim Pol. 06 Aralık 2018. doi:10.18388/abp.2018_2652
  50. Endres JR, Clewell A, Jade KA, Farber T, Hauswirth J, Schauss AG. Safety assessment of a proprietary preparation of a novel Probiotic, Bacillus coagulans, as a food ingredient. Food Chem Toxicol. Haziran 2009;47(6):1231-8. doi:10.1016/j.fct.2009.02.018
  51. Fares C, Menga V, Martina A, Pellegrini N, Scazzina F, Torriani S. Nutritional profile and cooking quality of a new functional pasta naturally enriched in phenolic acids, added with β-glucan and Bacillus coagulans GBI-30, 6086. J Cereal Sci. Eylül 2015;65:260-6. doi:10.1016/j.jcs.2015.07.017
  52. Saarela MH. Safety aspects of next generation probiotics. Curr Opin Food Sci. Aralık 2019;30:8-13. doi:10.1016/j.cofs.2018.09.001
  53. Celandroni F, Vecchione A, Cara A, Mazzantini D, Lupetti A, Ghelardi E. Identification of Bacillus species: Implication on the quality of probiotic formulations. Sharma V, editör. PLOS ONE. 20 Mayıs 2019;14(5):e0217021. doi:10.1371/journal.pone.0217021
  54. Green DH, Wakeley PR, Page A, Barnes A, Baccigalupi L, Ricca E, vd. Characterization of Two Bacillus Probiotics. Appl Environ Microbiol. Eylül 1999;65(9):4288-91. doi:10.1128/AEM.65.9.4288-4291.1999
  55. Lakshmi SG, Jayanthi N, Saravanan M, Ratna MS. Safety assesment of Bacillus clausii UBBC07, a spore forming probiotic. Toxicol Rep. 2017;4:62-71. doi:10.1016/j.toxrep.2016.12.004
  56. Bozdogan B, Galopin S, Gerbaud G, Courvalin P, Leclercq R. Chromosomal aadD2 Encodes an Aminoglycoside Nucleotidyltransferase in Bacillus clausii. Antimicrob Agents Chemother. Nisan 2003;47(4):1343-6. doi:10.1128/AAC.47.4.1343-1346.2003
  57. Bozdogan B, Galopin S, Leclercq R. Characterization of a New erm -Related Macrolide Resistance Gene Present in Probiotic Strains of Bacillus clausii. Appl Environ Microbiol. Ocak 2004;70(1):280-4. doi:10.1128/AEM.70.1.280-284.2004
  58. Galopin S, Cattoir V, Leclercq R. A chromosomal chloramphenicol acetyltransferase determinant from a probiotic strain of Bacillus clausii. FEMS Microbiol Lett. Temmuz 2009;296(2):185-9. doi:10.1111/j.1574-6968.2009.01633.x
  59. Anadón A, Rosa Martínez-Larrañaga M, Aranzazu Martínez M. Probiotics for animal nutrition in the European Union. Regulation and safety assessment. Regul Toxicol Pharmacol. Haziran 2006;45(1):91-5. doi:10.1016/j.yrtph.2006.02.004
  60. Li P, Tian W, Jiang Z, Liang Z, Wu X, Du B. Genomic Characterization and Probiotic Potency of Bacillus sp. DU-106, a Highly Effective Producer of L-Lactic Acid Isolated From Fermented Yogurt. Front Microbiol. 20 Eylül 2018;9:2216. doi:10.3389/fmicb.2018.02216
  61. Do Prado FG, Pagnoncelli MGB, De Melo Pereira GV, Karp SG, Soccol CR. Fermented Soy Products and Their Potential Health Benefits: A Review. Microorganisms. 09 Ağustos 2022;10(8):1606. doi:10.3390/microorganisms10081606
  62. Mah JH, Park Y, Jin Y, Lee JH, Hwang HJ. Bacterial Production and Control of Biogenic Amines in Asian Fermented Soybean Foods. Foods. 25 Şubat 2019;8(2):85. doi:10.3390/foods8020085
  63. Bader J, Albin A, Stahl U. Spore-forming bacteria and their utilisation as probiotics. Benef Microbes. 01 Mart 2012;3(1):67-75. doi:10.3920/BM2011.0039
  64. Li L, Liu B, Cao J, Zhang H, Tian F, Yu L, vd. Different effects of Bacillus coagulans vegetative cells and spore isolates on constipation-induced gut microbiota dysbiosis in mice. Food Funct. 2022;13(18):9645-57. doi:10.1039/D2FO01668K
  65. Tarik M, Ramakrishnan L, Bhatia N, Goswami R, Kandasamy D, Roy A, vd. The effect of Bacillus coagulans Unique IS-2 supplementation on plasma amino acid levels and muscle strength in resistance trained males consuming whey protein: a double-blind, placebo-controlled study. Eur J Nutr. Ağustos 2022;61(5):2673-85. doi:10.1007/s00394-022-02844-9
  66. Côté J, Dion J, Burguière P, Casavant L, Van Eijk J. Probiotics in Bread and Baked Products: A New Product Category. Cereal Foods World. Kasım 2013;58(6):293-6. doi:10.1094/CFW-58-6-0293
  67. Majeed M, Majeed S, Arumugam S, Ali F, Beede K. Comparative evaluation for thermostability and gastrointestinal survival of probiotic Bacillus coagulans MTCC 5856. Biosci Biotechnol Biochem. 24 Mart 2021;85(4):962-71. doi:10.1093/bbb/zbaa116
  68. Caccamo MT, Magazù S. An FT-IR Based Investigation of Trehalose Mediated Thermal Stabilisation of Bacillus clausii. Curr Nutr Food Sci. 09 Haziran 2021;17(6):566-71. doi:10.2174/1573401317666210210130042
  69. Mingmongkolchai S, Panbangred W. Bacillus probiotics: an alternative to antibiotics for livestock production. J Appl Microbiol. Haziran 2018;124(6):1334-46. doi:10.1111/jam.13690
  70. Urdaci MC, Bressollier P, Pinchuk I. Bacillus clausii Probiotic Strains: Antimicrobial and Immunomodulatory Activities. J Clin Gastroenterol. Temmuz 2004;38(Supplement 2):S86-90. doi:10.1097/01.mcg.0000128925.06662.69
  71. Sanders ME, Morelli L, Tompkins TA. Sporeformers as Human Probiotics: Bacillus, Sporolactobacillus, and Brevibacillus. Compr Rev Food Sci Food Saf. Temmuz 2003;2(3):101-10. doi:10.1111/j.1541-4337.2003.tb00017.x
  72. Gomes Da Cruz A, Alonso Buriti FC, Batista De Souza CH, Fonseca Faria JA, Isay Saad SM. Probiotic cheese: Health benefits, technological and stability aspects. Trends Food Sci Technol. Ağustos 2009;20(8):344-54. doi:10.1016/j.tifs.2009.05.001
  73. Guimarães JT, Balthazar CF, Silva R, Rocha RS, Graça JS, Esmerino EA, vd. Impact of probiotics and prebiotics on food texture. Curr Opin Food Sci. Haziran 2020;33:38-44. doi:10.1016/j.cofs.2019.12.002
  74. Miranda JS, Costa BV, De Oliveira IV, De Lima DCN, Martins EMF, De Castro Leite Júnior BR, vd. Probiotic jelly candies enriched with native Atlantic Forest fruits and Bacillus coagulans GBI-30 6086. LWT. Mayıs 2020;126:109275. doi:10.1016/j.lwt.2020.109275
  75. Ramos GD, Dias SLDS, Ferreira IM, Silva AMOE, Carvalho MGD. Cashew yogurt shelf life with Bacillus clausii: chemical, physicochemical and microbiological evaluation. Rev Bras Hig E Sanidade Anim. 2019;13(4). doi:10.5935/1981-2965.20190033
  76. Zeybekoğlu N, Özhan H, Boyacioğlu O. Probiotic Tofu with Bacillus clausii Spores to Support Gastrointestinal Microflora. Adnan Menderes Üniversitesi Sağlık Bilim Fakültesi Derg. 30 Eylül 2021;5(3):534-45. doi:10.46237/amusbfd.929382
  77. Refaey MM, Al-Amir EM, Zalma SA. Development of a Novel Multifunctional Probiotic Yogurt Fortified with Bacillus clausii and Carrot Powder with Enhanced Nutritional, Sensory, Rheological and Ultrastructural Properties. J Food Dairy Sci. 07 Mart 2026;0(0):35-49. doi:10.21608/jfds.2026.468571.1228
  78. Chelliah R, Kim NH, Rubab M, Yeon SJ, Barathikannan K, Vijayalakshmi S, vd. Robust and safe: Unveiling Bacillus clausii OHRC1’s potential as a versatile probiotic for enhanced food quality and safety. LWT. Temmuz 2024;203:116291. doi:10.1016/j.lwt.2024.116291
  79. Maity C, Bagkar P, Dixit Y, Tiwari A. Alkalihalobacillus clausii 088AE as a functional and medical food ingredient: assessment of in vitro protein digestibility and food calorie reduction. Int J Food Sci Technol. Haziran 2022;57(6):3440-55. doi:10.1111/ijfs.15668
  80. Joyce ONC, Chinweike OF, Godswill CC, Ifeanyi OO, Vivian IN, Christian SO. Production of Fermented Soymilk Drink Containing Probiotic Bacillus coagulans. Eur J Nutr Food Saf. 08 Kasım 2021;51-61. doi:10.9734/ejnfs/2021/v13i730436
  81. Cao J, Yu Z, Zhang Q, Yu L, Zhao J, Zhang H, vd. Effects of Bacillus coagulans GBI-30, 6086 as an adjunct starter culture on the production of yogurt. Food Res Int. Ekim 2022;160:111398. doi:10.1016/j.foodres.2022.111398
  82. Maity C, Bagkar P, Dixit Y, Tiwari A, Gupta AK. Process and Storage Stability of Bacillus coagulans LBSC in Food Matrices and Appraisal of Calorific Restriction: B. coagulans LBSC stability and calorie restriction. Appl Food Biotechnol. 29 Aralık 2020;8(1):57-69. doi:10.22037/afb.v8i1.31212
  83. Sreenadh M, Kumar KR, Nath S. In Vitro Evaluation of Weizmannia coagulans Strain LMG S-31876 Isolated from Fermented Rice for Potential Probiotic Properties, Safety Assessment and Technological Properties. Life. 06 Eylül 2022;12(9):1388. doi:10.3390/life12091388
  84. Xu L, Zhan ZC, Du S, Wang S, Zhang Q, Wang C, vd. Isolation, functional evaluation, and fermentation process optimization of probiotic Bacillus coagulans. El Basuini MF, editör. PLOS ONE. 03 Kasım 2023;18(11):e0286944. doi:10.1371/journal.pone.0286944
  85. Yoon HJ, Lee KA, Lee NK, Jang HJ, Paik HD. Isolation of Bacillus spp. from Korean fermented foods and their application in black rice bran fermentation. Food Eng Prog. Kasım 2024;28(4):289-95. doi:10.13050/foodengprog.2024.28.4.289
  86. Lim H, Oh S, Yu S, Kim M. Isolation and Characterization of Probiotic Bacillus subtilis MKHJ 1-1 Possessing L-Asparaginase Activity. Appl Sci. 14 Mayıs 2021;11(10):4466. doi:10.3390/app11104466
  87. Feng Y, Gao Y, Cui L, Feng J, Fan Y, Zhang Y, vd. Enhancing natto quality by co-fermentation with Pediococcus pentosaceus PP04 and Bacillus velezensis BV702: Nattokinase activity, nutritional profile, and flavor characteristics. Food Chem X. Ocak 2026;33:103430. doi:10.1016/j.fochx.2025.103430
  88. Hu M, Jiao R, Zhang X, Li H, Ren Y, Wang Y, vd. Isolation and safety evaluation of food-derived potential probiotic Bacillus with antimicrobial properties. Microb Pathog. Nisan 2025;201:107338. doi:10.1016/j.micpath.2025.107338
  89. Peng S, Guo C, Zhang X, Bu X, Li X, Cui H, vd. Kinetic study of the thermal inactivation of Weizmannia coagulans during food thermal processing. Heliyon. Eylül 2024;10(17):e36977. doi:10.1016/j.heliyon.2024.e36977
  90. Can K, Bozatlı SB, Dikici A. Survival of two probiotic Bacillaceae during processing, storage, and digestion of meat products. Npj Sci Food. 21 Mayıs 2026. doi:10.1038/s41538-026-00883-8
  91. De Paula Antunes DJ, De Almeida Costa N, Martins ML, Benevenuto WCADN, Talma SV, Campos ANDR, vd. Healthy cereal bar enriched with probiotic Bacillus: bromatological characterization, probiotic viability, consumer acceptance, and gastrointestinal resistance in vitro. Int J Food Sci Technol. 06 Ocak 2025;60(1):vvae070. doi:10.1093/ijfood/vvae070
  92. Sanders ME, Morelli L, Tompkins TA. Sporeformers as Human Probiotics: Bacillus, Sporolactobacillus, and Brevibacillus. Compr Rev Food Sci Food Saf. Temmuz 2003;2(3):101-10. doi:10.1111/j.1541-4337.2003.tb00017.x
  93. De Paula Antunes DJ, De Almeida Costa N, Martins ML, Benevenuto WCADN, Talma SV, Campos ANDR, vd. Healthy cereal bar enriched with probiotic Bacillus: bromatological characterization, probiotic viability, consumer acceptance, and gastrointestinal resistance in vitro. Int J Food Sci Technol. 06 Ocak 2025;60(1):vvae070. doi:10.1093/ijfood/vvae070