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Journals / Turkish Journal of Biodiversity / 2024 / Cilt: 7 Sayı: 2

Determination of the volatile, phenolic and fatty acid contents of Helichrysum plicatum by chromatographic methods

Helichrysum plicatum’un uçucu, fenolik ve yağ asidi içeriklerinin kromatografik yöntemlerle belirlenmesi

Yunus Başar(IGDIR UNIVERSITY)0000-0002-7785-3242
Fatih Gül(IĞDIR ÜNİVERSİTESİ, IĞDIR MESLEK YÜKSEKOKULU)0000-0002-4297-786X
Musa Karadağ(IGDIR UNIVERSITY)0000-0003-2498-3403
Mehmet Hakkı Alma(IGDIR UNIVERSITY)0000-0001-6323-7230
İbrahim Demirtas(IGDIR UNIVERSITY)0000-0001-8946-647X
Ahmet Zafer Tel(IGDIR UNIVERSITY)0000-0002-1204-3839
Pages
83–94
Publication Date
2024-12-31
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Therefore, its use in food, cosmetics and pharmacology can be widespread.

📌 3 Key Takeaways

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Objective & Hypothesis:Helichrysum plicatum, which is widely distributed in Anatolia, is known for its antidiabetic, spasmolytic, nephroprotective and antimutagenic properties and is used in traditional medicine to heal wounds and treat stomach, liver, diabetes and kidney stones.
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Key Findings & Data:Caryophyllene (17.83%) and α-pinene (16.69%) were detected in high amounts in GC-MS/MS analysis of the essential oil.
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Conclusion & Impact:Therefore, its use in food, cosmetics and pharmacology can be widespread.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Helichrysum pilicatum · fenolik · fitokimyasal analiz

Abstract

Helichrysum plicatum, which is widely distributed in Anatolia, is known for its antidiabetic, spasmolytic, nephroprotective and antimutagenic properties and is used in traditional medicine to heal wounds and treat stomach, liver, diabetes and kidney stones. It is assumed that the biological activities of H. plicatum are due to the bioactive components it contains. In this study, the volatile oil substances, phenols and fatty acid contents of H. pilicatum were determined by chromatographic methods. Three different extracts were obtained: ethanol, ethanol-water and methanol-chloroform. Caryophyllene (17.83%) and α-pinene (16.69%) were detected in high amounts in GC-MS/MS analysis of the essential oil. Palmitic acid methyl ester (14.74-26.88%), oleic acid methyl ester (12.23-23.38%), lauric acid methyl ester (17.88-19.91%), octacosane (4.55-17.00%) and stearic acid methyl ester (4.3-14.81%) were also determined as the main components in the analysis of fatty acid content of the extracts. In the phenolic content of the extracts, naringenin and vanillic acid were determined in high amounts in ethanol and ethanol-water extracts, resveratrol and chlorogenic acid in methanol-chloroform extracts. Based on these compounds determined and known to have biological activity, their use in traditional medicine and many other areas was confirmed. It is expected that this study will shed light on in vivo and in vitro studies for detailed phytochemical analysis of H. plicatum plant. Therefore, its use in food, cosmetics and pharmacology can be widespread.

Özet

Anadolu'da yaygın olarak bulunan Helichrysum plicatum, antidiyabetik, spazmolitik, nefroprotektif ve antimutajenik özellikleriyle bilinir ve geleneksel tıpta yaraları iyileştirmek ve mide, karaciğer, diyabet ve böbrek taşlarını tedavi etmek için kullanılır. H. plicatum'un biyolojik aktivitelerinin içerdiği biyoaktif bileşenlerden kaynaklandığı varsayılmaktadır. Bu çalışmada H. pilicatum'un uçucu yağ maddeleri, fenoller ve yağ asidi içerikleri kromatografik yöntemlerle belirlendi. Üç farklı ekstrakt elde edildi: etanol, etanol-su ve metanol-kloroform. Uçucu yağın GC-MS/MS analizinde karyofilen (%17.83) ve α-pinen (%16.69) yüksek miktarlarda tespit edildi. Ekstraktların yağ asidi içeriğinin analizinde ayrıca palmitik asit metil ester (%14.74-26.88), oleik asit metil ester (%12.23-23.38), laurik asit metil ester (%17.88-19.91), oktakosan (%4.55-17.00) ve stearik asit metil ester (%4.3-14.81) ana bileşenler olarak belirlendi. Ekstraktların fenolik içeriğinde etanol ve etanol-su ekstraktlarında yüksek miktarlarda naringenin ve vanilik asit, metanol-kloroform ekstraktlarında ise resveratrol ve klorojenik asit belirlenmiştir. Biyolojik aktivitesi olduğu belirlenen ve bilinen bu bileşiklere dayanarak geleneksel tıpta ve diğer birçok alanda kullanımları doğrulanmıştır. Bu çalışmanın H. plicatum bitkisinin detaylı fitokimyasal analizi için in vivo ve in vitro çalışmalara ışık tutması beklenmektedir. Bu nedenle gıda, kozmetik ve farmakolojide kullanımı yaygınlaştırılabilir.

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Cite This Article

Başar, Y., Gül, F., Karadağ, M., Alma, M. H., Demirtas, İ., Tel, A. Z. (2024). Determination of the volatile, phenolic and fatty acid contents of Helichrysum plicatum by chromatographic methods. Turkish Journal of Biodiversity, Cilt: 7 Sayı: 2, 83–94. https://doi.org/10.38059/biodiversity.1600406

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