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Journals / Turkish Journal of Analytical Chemistry / 2026 / Cilt: 7 Sayı: 2

Validation of stability-indicating high-performance liquid chromatography method for the determination of thymol in gelatin-based hydrogels

Jelatin bazlı hidrojellerde timolün tayini için stabilite göstergeli yüksek performanslı sıvı kromatografisi yönteminin doğrulanması

Onur Demir(Pendik Veteriner Kontrol Enstitüsü)0000-0001-9076-3455
Mehlika Pulat(GAZİ ÜNİVERSİTESİ, FEN FAKÜLTESİ, KİMYA BÖLÜMÜ)0000-0001-5724-5250
Ali Bilgili(ANKARA UNIVERSITY, ANKARA FACULTY OF VETERINARY MEDICINE)0000-0001-6819-7952
Pages
132–139
Publication Date
2025-05-31
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This study presents a sensitive, convenient and practical method for the detection of thymol in gelatin-based hydrogel structure and model release atmosphere.

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of this study was to develop an analytical method to determine the amount of thymol loaded into the structure of a gelatin hydrogel developed to struggle varroosis infestation of honey bees and released into a model release system.
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Key Findings & Data:Chromatographic separation was achieved using a high-performance liquid chromatograph, C18 column and acetonitrile:water (75:25) mobile phase with an isocratic flow rate of 1 mL/min.
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Conclusion & Impact:This study presents a sensitive, convenient and practical method for the detection of thymol in gelatin-based hydrogel structure and model release atmosphere.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Kontrollü salım · HPLC · hidrojel

Abstract

The aim of this study was to develop an analytical method to determine the amount of thymol loaded into the structure of a gelatin hydrogel developed to struggle varroosis infestation of honey bees and released into a model release system. Chromatographic separation was achieved using a high-performance liquid chromatograph, C18 column and acetonitrile:water (75:25) mobile phase with an isocratic flow rate of 1 mL/min. To validate the method, specificity, precision, linearity, detection and measurement limit, accuracy, robustness, solution stability and system suitability parameters were studied. Hydrolytic, thermal, oxidative and photolytic degradation studies were performed for stress testing. The method is linear in the range of 0.25-15 μg/mL (R2 = 0.999). LOD was calculated as 6.2261 ng/mL and LOQ as 18.866 ng/mL. Intra-day and inter-day precision study %RSD values were ≤ 2%. The average recovery for the hydrogel was 100.3% (±2.12) and for the air sample was 99.9% (±3.14). The analytical method proved to be specific, linear, precise and robust. This study presents a sensitive, convenient and practical method for the detection of thymol in gelatin-based hydrogel structure and model release atmosphere.

Özet

Bu çalışmanın amacı, bal arılarındaki varroosis istilasıyla mücadele için geliştirilen bir jelatin hidrojelin yapısına yüklenen ve bir model salım sistemine salınan timol miktarını belirlemek için analitik bir yöntem geliştirmektir. Kromatografik ayırma, yüksek performanslı sıvı kromatografı, C18 kolon ve asetonitril:su (75:25) hareketli faz kullanılarak, 1 mL/dakika izokratik akış hızıyla gerçekleştirildi. Yöntemi doğrulamak için özgüllük, hassasiyet, doğrusallık, tespit ve ölçüm limiti, doğruluk, sağlamlık, çözelti stabilitesi ve sistem uygunluk parametreleri incelendi. Stres testi için hidrolitik, termal, oksidatif ve fotolitik bozunma çalışmaları yapıldı. Yöntem, 0,25-15 μg/mL (R2 = 0,999) aralığında doğrusaldır. LOD, 6,2261 ng/mL ve LOQ, 18,866 ng/mL olarak hesaplandı. Gün içi ve günler arası hassasiyet çalışması %RSD değerleri ≤ %2 idi. Hidrojel için ortalama geri kazanım %100,3 (±2,12) ve hava örneği için %99,9 (±3,14) idi. Analitik yöntemin özgül, doğrusal, kesin ve sağlam olduğu kanıtlandı. Bu çalışma, jelatin bazlı hidrojel yapısında ve model salım atmosferinde timolün tespiti için hassas, kullanışlı ve pratik bir yöntem sunmaktadır.

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Cite This Article

Demir, O., Pulat, M., Bilgili, A. (2025). Validation of stability-indicating high-performance liquid chromatography method for the determination of thymol in gelatin-based hydrogels. Turkish Journal of Analytical Chemistry, Cilt: 7 Sayı: 2, 132–139. https://doi.org/10.51435/turkjac.1604250

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