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Journals / Turkish Computational and Theoretical Chemistry / 2026 / Volume: 9 Issue: 4

Metagenomic analysis’ rhizomicrobiome’ Prunus mahaleb based on 16S RNA gene sequencing on the Illumina MiSeq

Serap Çetinkaya(CUMHURIYET UNIVERSITY)0000-0001-7372-1704
Aysun Baş(SIVAS CUMHURIYET UNIVERSITY)0000-0001-9168-2745
Muhammed Safa Çelik(SIVAS CUMHURIYET UNIVERSITY)0000-0001-7226-5268
Hüseyin Fatih Çetinkaya(CUMHURIYET UNIVERSITY)0000-0001-6338-3414
Burak Tüzün(SİVAS CUMHURİYET ÜNİVERSİTESİ, SİVAS TEKNİK BİLİMLER MESLEK YÜKSEKOKULU)0000-0002-0420-2043
Pages
—
Publication Date
2025-12-15
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

A thorough insight into the rhizomicrobiome is therefore envisaged to facilitate the development of microbial fertilizers to improve plant performance and productivity impacting eventually food security.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The study covered for the first time the investigation of the microbiome of Prunus mahaleb rhizosphere.
📊
Key Findings & Data:The methodology included both metagenomic and bioinformatic means, and used the bidirectional sequencing of V3 region of 16S rRNA gene with oligo-primers universal to both Bacteria and Archaea.
💡
Conclusion & Impact:A thorough insight into the rhizomicrobiome is therefore envisaged to facilitate the development of microbial fertilizers to improve plant performance and productivity impacting eventually food security.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Bioinformatics · metagenomics · Illumina MiSeq sequencing

Abstract

The study covered for the first time the investigation of the microbiome of Prunus mahaleb rhizosphere. The methodology included both metagenomic and bioinformatic means, and used the bidirectional sequencing of V3 region of 16S rRNA gene with oligo-primers universal to both Bacteria and Archaea. Results of Illumina MiSeq sequencing indicated the presence of 49 phyla, 104 classes, 242 orders, 353 families and 761 genera. The commonest genus was, not surprisingly, Pseudomonas. This genus was closely followed by uncultured genera and this finding was considered to have very meaningful implications as to the functional aspects of a biome and the importance of it was discussed in the text. A thorough insight into the rhizomicrobiome is therefore envisaged to facilitate the development of microbial fertilizers to improve plant performance and productivity impacting eventually food security.

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Cite This Article

Çetinkaya, S., Baş, A., Çelik, M. S., Çetinkaya, H. F., Tüzün, B. (2025). Metagenomic analysis’ rhizomicrobiome’ Prunus mahaleb based on 16S RNA gene sequencing on the Illumina MiSeq. Turkish Computational and Theoretical Chemistry, Volume: 9 Issue: 4. https://doi.org/10.33435/tcandtc.1714834

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