Journals / Sabuncuoglu Serefeddin Health Sciences / 2026 / Cilt: 8 Sayı: 2

DETERMINATION OF THE BIOACTIVE COMPOSITION AND ANTIMICROBIAL ACTIVITY OF THE AQUEOUS EXTRACT OF WILD BLUEBERRIES COLLECTED FROM KURT DAĞI HIGHLANDS (TRABZON, TÜRKİYE)

KURT DAĞI YAYLASI’NDAN (TRABZON, TÜRKİYE) TOPLANAN YABAN MERSİNİ MEYVESİNİN SULU EKSTRAKTININ BİYOAKTİF İÇERİĞİNİN VE ANTİMİKROBİYAL AKTİVİTESİNİN BELİRLENMESİ

Hanife Kara(AMASYA ÜNİVERSİTESİ)0000-0002-1803-7237
Salih Özden(AMASYA ÜNİVERSİTESİ, SABUNCUOĞLU ŞEREFEDDİN SAĞLIK HİZMETLERİ MESLEK YÜKSEKOKULU, TIBBİ HİZMETLER VE TEKNİKLER BÖLÜMÜ)0000-0002-2248-825X
Meltem Özden(AMASYA ÜNİVERSİTESİ, SABUNCUOĞLU ŞEREFEDDİN SAĞLIK HİZMETLERİ MESLEK YÜKSEKOKULU, TIBBİ HİZMETLER VE TEKNİKLER BÖLÜMÜ)0000-0002-1815-2577
Pages
90–98
Publication Date
2026-08-31
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These data indicate that the blueberry population in the region has a significant antimicrobial potential, consistent with its high phenolic content, and can be considered a natural inhibitor of pathogenic microorganisms.

📌 3 Key Takeaways

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Objective & Hypothesis:Blueberries (Vaccinium arctostaphylos L.), which grow naturally in the Black Sea region and are consumed by the local population for their health benefits, are known as a functional food characterized by small, dark purple-blue berries.
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Key Findings & Data:A single pooled aqueous extract derived from Vaccinium arctostaphylos fruits (n=1) was prepared: floral samples collected from the Kurt Dağı plateau in August 2024 were dried at 45°C for 24 hours; a 700 mg portion of this sample was dissolved in 25 mL of pure water, and all measurements were performed on the resulting solution.
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Conclusion & Impact:These data indicate that the blueberry population in the region has a significant antimicrobial potential, consistent with its high phenolic content, and can be considered a natural inhibitor of pathogenic microorganisms.
🔬Design:Lab & Field Experiment
📍Scope:2024 Verileri
⚙️Variables:Antimikrobiyal · Yaban Mersini · Flavonoidler

Abstract

Blueberries (Vaccinium arctostaphylos L.), which grow naturally in the Black Sea region and are consumed by the local population for their health benefits, are known as a functional food characterized by small, dark purple-blue berries. Considering that the bioactive content and health effects of blueberries may vary depending on the environmental conditions in which they are grown, we aimed to measure the polyphenol and flavonoid contents of blueberries naturally grown on the Kurt Dağı plateau and to determine their antimicrobial activity. A single pooled aqueous extract derived from Vaccinium arctostaphylos fruits (n=1) was prepared: floral samples collected from the Kurt Dağı plateau in August 2024 were dried at 45°C for 24 hours; a 700 mg portion of this sample was dissolved in 25 mL of pure water, and all measurements were performed on the resulting solution. The extraction process and chemical measurements were repeated three times. The total polyphenol (21.041 ± 0.976 µg/g GAE) and flavonoid content (1.733 ± 0.294 µg/g QE) were quantified. Antimicrobial susceptibility testing against three standard reference strains revealed that the pooled extract exhibited moderate-weak inhibitory activity against Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922 (MIC: 6.5 mg/mL), whereas only weak inhibitory activity was observed against Pseudomonas aeruginosa ATCC 27853 (MIC: 25 mg/mL). These data indicate that the blueberry population in the region has a significant antimicrobial potential, consistent with its high phenolic content, and can be considered a natural inhibitor of pathogenic microorganisms.

Özet

Karadeniz bölgesinde doğal olarak yetişen ve yerel halk tarafından sağlık açısından faydalı etkisi yüzünden tüketilen yaban mersini (Vaccinium arctostaphylos L.) küçük koyu mor/mavi renkli meyvelere sahip fonksiyonel bir gıda olarak bilinmektedir. Yaban mersininin yetiştiği çevre koşullarına göre, biyoaktif içeriği ve sağlık üzerine etkisinin farklılık gösterebileceğini göz önüne alarak; Kurt Dağı yaylasında doğal ortamda yetişen yaban mersininin polifenol ve flavonoid içeriğini ölçmek ve buna bağlı olarak antimikrobiyal aktivitesini belirlemeyi amaçladık. Kurt Dağı platosundan Ağustos 2024’te toplanan meyve örneklerinden (n=1) tek bir birleşik sulu ekstrakt hazırlanmıştır. Örnekler 45°C’de 24 saat boyunca kurutulmuş; bu örnekten 700 mg’lık bir kısım 25 mL saf su içinde çözülmüş ve tüm ölçümler elde edilen çözelti üzerinde gerçekleştirilmiştir. Ekstraksiyon işlemi ve kimyasal ölçümler üç kez tekrarlanmıştır. Toplam polifenol içeriği (21.041 ± 0.976 µg/g GAE) ve flavonoid içeriği (1.733 ± 0.294 µg/g QE) kantitatif olarak belirlenmiştir. Üç standart referans suşa karşı yapılan antimikrobiyal duyarlılık testleri, birleşik sulu ekstraktın Staphylococcus aureus ATCC 25923 ve Escherichia coli ATCC 25922’ye karşı orta-zayıf düzeyde inhibitör aktivite gösterdiğini (MİK: 6.5 mg/mL), buna karşın Pseudomonas aeruginosa ATCC 27853’e karşı yalnızca zayıf inhibitör aktivite sergilediğini ortaya koymuştur (MİK: 25 mg/mL). Elde edilen bu veriler, bölgedeki yaban mersini popülasyonunun yüksek fenolik madde içeriğiyle paralel olarak kayda değer bir antimikrobiyal potansiyele sahip olduğunu ve patojen mikroorganizmalara karşı doğal bir inhibitör ajan olarak değerlendirilebileceğini göstermektedir.

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Cite This Article

Kara, H., Özden, S., Özden, M. (2026). DETERMINATION OF THE BIOACTIVE COMPOSITION AND ANTIMICROBIAL ACTIVITY OF THE AQUEOUS EXTRACT OF WILD BLUEBERRIES COLLECTED FROM KURT DAĞI HIGHLANDS (TRABZON, TÜRKİYE). Sabuncuoglu Serefeddin Health Sciences, Cilt: 8 Sayı: 2, 90–98. https://doi.org/10.55895/sshs.1941273

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