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Journals / Ege Üniversitesi Diş Hekimliği Fakültesi / 2018 / Cilt: 39 - Sayı: 2

Analyses of Effect of New Hemostatic Agent Mecsina Hemostopper® on Gingival Fibroblast Proliferation and Evaluation of Cytotoxicity by Different Tests

Yeni Bir Hemostatik Ajan Olan Mecsina Hemostopper® ‘ın Farklı Testlerle Sitotoksisitesinin Değerlendirilip, Gingival Fibroblast Proliferasyonuna Etkisinin Araştırılması

MEHMET KEMAL TÜMER
MUSTAFA ÇİÇEK
Pages
105–110
DOI
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⚡ TL;DR(Too Long; Didn't Read)

DISCUSSION AND CONCLUSION: This study showed that Mecsina Hemostopper has different cytotoxic levels at different concentrations in the gingival fibroblast cell lines.

📌 3 Key Takeaways

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Objective & Hypothesis:INTRODUCTION: In this study, the effects of the mechanism of action of "Mecsina Hemostopper®", a new generation anti-hemorrhagic agent, on fibroblast cell proliferation was investigated by using XTT (2,3-Bis- (2-Methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium) cytotoxicity assay.
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Key Findings & Data:The cells, 5000 cells per flask for each different dose group, were distributed into the 9 groups (mecsina 1/1, 1/2, 1/10, 1/50, 1/100, 1/200, 1/500, distilled water-administered negative and control without any administration).
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Conclusion & Impact:DISCUSSION AND CONCLUSION: This study showed that Mecsina Hemostopper has different cytotoxic levels at different concentrations in the gingival fibroblast cell lines.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

INTRODUCTION: In this study, the effects of the mechanism of action of "Mecsina Hemostopper®", a new generation anti-hemorrhagic agent, on fibroblast cell proliferation was investigated by using XTT (2,3-Bis- (2-Methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium) cytotoxicity assay. METHODS: The immortalized human gingival fibroblast cell lines were commercially purchased for the study. The cells, 5000 cells per flask for each different dose group, were distributed into the 9 groups (mecsina 1/1, 1/2, 1/10, 1/50, 1/100, 1/200, 1/500, distilled water-administered negative and control without any administration). After the incubation for 24 hours, the cytotoxicity values were measured by XTT analysis technique for each group. RESULTS: There was a significant difference in the effects of Mecsina Hemostopper on fibroblast cells between the dose groups (p 0,05). DISCUSSION AND CONCLUSION: This study showed that Mecsina Hemostopper has different cytotoxic levels at different concentrations in the gingival fibroblast cell lines.

Özet

GİRİŞ ve AMAÇ: Sağlık alanında hali hazırda aktif olarak kullanılan anti-hemorajik ajanlar farklı etki mekanizmaları kullanarak kanamayı engelleyebilir. Bu çalışmada yeni nesil bir anti-hemorajik ajan olan “Mecsina Hemostopper®”ın etki mekanizmasının, XTT (2,3-Bis(2-metoksi4-nitro-5-sulfofenil)-2Htetrazolyum) sitotoksisite analiz metodu ile fibroblast hücre proliferasyonu üzerine etkileri araştırılmıştır. YÖNTEM ve GEREÇLER: Çalışma için insan gingival fibroblast ölümsüz hücre hatları ticari olarak satın alındı. Her flaska her bir farklı doz grubu için 5000 hücre olacak şekilde 9 gruba (Mecsina Hemostopper 1/1, Mecsina Hemostopper 1/2, Mecsina Hemostopper 1/10, Mecsina Hemostopper 1/50, Mecsina Hemostopper 1/100, Mecsina Hemostopper 1/200, Mecsina Hemostopper 1/500, distile su uygulanan negative ve hiçbir sey uygulanmayan kontrol ) hücreler dağıtıldı. 24 saat inkübasyondan sonra her bir grup için XTT analiz yöntemi ile sitotoksisite değerleri ölçülmüştür. BULGULAR: Mecsina Hemostopper’ın fibroblast hücresine olan etkilerinin doz grupları arasında anlamlı fark görülmüştür (p 0,05). TARTIŞMA ve SONUÇ: Bu çalışmada, yeni nesil kanama durdurucu olan mecsina hemostopper gingival fibroblast hücre hatlarında farklı konsantrasyonlarda farklı derecede sitotoksik değerleri olduğu saptanmıştır.

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Cite This Article

TÜMER, M. K., ÇİÇEK, M. (2018). Analyses of Effect of New Hemostatic Agent Mecsina Hemostopper® on Gingival Fibroblast Proliferation and Evaluation of Cytotoxicity by Different Tests. Ege Üniversitesi Diş Hekimliği Fakültesi, Cilt: 39 - Sayı: 2, 105–110. https://ajindex.com/articles/153477

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