Journals / Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi / 2026 / Cilt: 8 Sayı: 2

Essential Oil Composition, Antioxidant Activity, and Phenolic Content of Melampyrum Arvense L. Var. Arvense L.

Melampyrum arvense L. var. arvense L.'nin Uçucu Yağ Bileşimi, Antioksidan Aktivitesi ve Fenolik İçeriği

Azize Demirpolat(BİNGÖL ÜNİVERSİTESİ)0000-0001-7192-185X
Piroz Düzdaban(BITLIS EREN UNIVERSITY)0000-0002-4686-9030
Dincer Cobanoglu(BİNGÖL ÜNİVERSİTESİ)0009-0004-9511-869X
Muhammad Zafar(Quaid-e-Azam University, Islamabad)0000-0002-7440-0635
Mustaq Ahmad(Quaid Azam University)0000-0002-0705-4322
Ömer Kılıç(ADIYAMAN ÜNİVERSİTESİ)0000-0003-3409-1572
Pages
490–504
Publication Date
2025-03-12
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

As a result of this study, high levels of fatty acids were detected in M. arvense var. arvense essential oil, which has not been reported in the previous literature.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, the essential oil composition, antioxidant activity, and phenolic content of Melampyrum arvense L. var. arvense L. were investigated.The aerial part of M. arvense was hydrodistilled, and a light yellowish oil yield of 0.9% (v/w) was obtained.
📊
Key Findings & Data:According to GC/MS analysis, 70 compounds were identified in the oil of M. arvense var arvense.
💡
Conclusion & Impact:As a result of this study, high levels of fatty acids were detected in M. arvense var. arvense essential oil, which has not been reported in the previous literature.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:M. arvense var. arvense · LC-MS/MS · Uçucu yağ

Abstract

In this study, the essential oil composition, antioxidant activity, and phenolic content of Melampyrum arvense L. var. arvense L. were investigated.The aerial part of M. arvense was hydrodistilled, and a light yellowish oil yield of 0.9% (v/w) was obtained. Essential oil composition analysed with GC-MS. Methanolic extracts were prepared from whole parts of M. arvense var. arvense to determine the antioxidant activity and phenolic content was detected with LC-MS/MS. According to GC/MS analysis, 70 compounds were identified in the oil of M. arvense var arvense. The major compounds in M. arvense var. arvense were n-hexadecanoic acid-palmitic (17.73%), phenol, 2,4-bis (1,1-dimethylethyl) (15.56%), and octadecanoic acid-stearic acid (9.96%). Total phenolic and flavonoid content, DPPH•, and ABTS•+ scavenging activities were 33.5±2.6 mg GAE/g dw, 76.5±1.1 mg QE/g dw, 21.5±2.7 mg Trolox/g dw, and 30.7±0.45 mg Trolox/g dw, respectively. The most prevalent phenolic compound was 4-OH-benzoic acid. As a result of this study, high levels of fatty acids were detected in M. arvense var. arvense essential oil, which has not been reported in the previous literature.

Özet

Bu çalışmada, Melampyrum arvense L. var. arvense L.'nin uçucu yağ bileşimi, antioksidan aktivitesi ve fenolik içeriği araştırılmıştır. M. arvense'nin toprak üstü kısmı hidrodistile edilmiş ve %0,9 (v/w) açık sarımsı yağ verimi elde edilmiştir. Uçucu yağ bileşimi GC-MS ile analiz edilmiştir. Antioksidan aktiviteyi belirlemek için M. arvense var. arvense'nin tüm kısımlarından metanolik ekstraktlar hazırlanıp ve fenolik içerik LC-MS/MS ile tespit edilmiştir. GC/MS analizine göre, M. arvense var. arvense yağında 70 bileşik tanımlanmıştır. M. Arvense var. arvense'deki başlıca bileşikler n-hekzadekanoik asit-palmitik (%17,73), fenol, 2,4-bis (1,1-dimetiletil) (%15,56) ve oktadekanoik asit-stearik asit (%9,96) idi. Toplam fenolik ve flavonoid içeriği, DPPH- ve ABTS-+ süpürme aktiviteleri sırasıyla 33.5±2.6 mg GAE/g dw, 76.5±1.1 mg QE/g dw, 21.5±2.7 mg Trolox/g dw ve 30.7±0.45 mg Trolox/g dw olarak tespit edilmiştir. En yaygın fenolik bileşik 4-OH-benzoik asit olmuştur. Bu çalışma sonucunda, M. arvense var. arvense uçucu yağında daha önceki literatürde rapor edilmemiş olan yüksek seviyelerde yağ asitleri tespit edilmiştir.

🔖

Cite This Article

Demirpolat, A., Çobanoğlu, D. N., Düzdaban, P., Cobanoglu, D., Zafar, M., Ahmad, M., Kılıç, Ö. (2025). Essential Oil Composition, Antioxidant Activity, and Phenolic Content of Melampyrum Arvense L. Var. Arvense L.. Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Cilt: 8 Sayı: 2, 490–504. https://doi.org/10.47495/okufbed.1496786

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (43)

  1. Abdullah ASH., Mirghani MES., Jamal P. Antibacterial activity of Malaysian mango kernel. African Journal of Biotechnology 2011; 10(81): 18739-18748.
  2. Agoramoorthy G., Chandrasekaran M., Venkatesalu V., Hsu MJ. Antibacterial and antifungal activities of fatty acid methyl esters of the blind-your-eye mangrove from India. Brazilian Journal of Microbiology 2007; 38: 739-742.
  3. Akman F., Demirpolat A., Kazachenko AS., Kazachenko AS., Issaoui N., Al-Dossary O. Molecular structure, electronic properties, reactivity (elf, lol, and fukui), and ncı-rdg studies of the binary mixture of water and essential oil of Phlomis bruguieri. Molecules 2022; 28(6): 2684.
  4. Babbar N., Oberoi HS., Sandhu SK. Therapeutic and nutraceutical potential of bioactive compounds extracted from fruit residues. Critical Reviews in Food Science and Nutrition 2015; 55(3): 319-337.
  5. Barros L., Calhelha RC., Vaz JA., Ferreira ICFR., Baptista P, Estevinho LM. Antimicrobial activity and bioactive compounds of Portuguese wild edible mushrooms methanolic extracts. European Food Research and Technology 2007; 225(2):151-156.
  6. Bendary E., Francis RR., Ali HG., Sarwat MI., El Hady S. Antioxidant and structure-activity relationships (SARs) of some phenolic and anilines compounds. Annals of Agricultural Sciences 2013; 58(2): 173-181.
  7. Brand-Williams W., Cuvelier ME., Berset C. Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology 1995; 28(1): 25-30.
  8. Choi Y., Lee J. Antioxidant and antiproliferative properties of a tocotrienol-rich fraction from grape seeds. Food Chemistry 2009; 114(4): 1386-1390.
  9. Çobanoğlu DN., Şeker ME., Temizer İK., Erdoğan A. Investigation of botanical origin, phenolic compounds, carotenoids, and antioxidant properties of monofloral and multifloral bee bread. Chemistry & Biodiversity 2023; 20(3): 1124.
  10. Côté J., Caillet S., Doyon G., Sylvain JF., Lacroix M. Bioactive compounds in cranberries and their biological properties. Critical Reviews in Food Science and Nutrition 2010; 50(7): 666-679.
  11. Dalrymple SE. Biological flora of the British Isles: Melampyrum sylvaticum L. Journal of Ecology 2007; 95(3): 583-597.
  12. Demirpolat A. Chemical composition of essential oils of seven Polygonum species from Turkey: A chemotaxonomic approach. Molecules 2022; 27(24): 9053.
  13. Galishevskaya EE., Petrichenko VM. Phenolic compounds from two Melampyrum species. Pharmaceutical Chemistry Journal 2010; 44(9): 497-500.
  14. Gao H., Li P., Xu X., Zeng Q., Guan W. Research on volatile organic compounds from Bacillus subtilis CF-3: Biocontrol effects on fruit fungal pathogens and dynamic changes during fermentation. Frontiers in Microbiology 2018; 9: 309082.
  15. Gopinathan M., Balasubramanian M. Correlation analysis of phytochemicals and antioxidant activities of Heliotropium indicum L. leaves extract. International Journal of Pharmaceutical Sciences and Research 2021; 12(8): 4386-4393.
  16. Háznagy-Radnai E., Wéber E., Czigle S., Berkecz R., Csed K., Hohmann J. Identification of iridoids, flavonoids and triterpenes from the methanolic extract of Melampyrum bihariense A. Kern. and the antioxidant activity of the extract. Chromatographia 2014; 77(17-18): 1153-1159.
  17. Johnson J., Collins T., Power A., Chandra S., Skylas D., Portman D., Panozzo J., Blanchard C., Naiker M. Antioxidative properties and macrochemical composition of five commercial mungbean varieties in Australia. Legume Science 2020; 2(1): 1-11.
  18. Kadoma Y., Ito S., Atsumi T., Fujisawa S. Mechanisms of cytotoxicity of 2- or 2,6-di-tert-butylphenols and 2-methoxyphenols in terms of inhibition rate constant and a theoretical parameter. Chemosphere 2009; 74(5): 626-632.
  19. Karadağ AE., Tosun F. In vitro antimicrobial and antioxidant activity evaluation of Melampyrum arvense L. var. elatius Boiss. and Sedum spurium Bieb. extracts. Acta Pharmaceutica Sciencia 2019; 57(2): 193-201.
  20. Karthick P., Mohanraju R. Antimicrobial potential of epiphytic bacteria associated with seaweeds of little Andaman, India. Frontiers in Microbiology2018; 9: 291712.
  21. Kim YH., Park SK., Hur JY., Kim YC. Purification and characterization of a major extracellular chitinase from a biocontrol bacterium, Paenibacillus elgii HOA73. Plant Pathology Journal 2017; 33(3): 318-328.
  22. Kirmizibekmez H., Atay I., Kaiser M., Brun R., Cartagena MM., Carballeira NM., Yesilada E, Tasdemir D. Antiprotozoal activity of Melampyrum arvense and its metabolites. Phytotherapy Research 2011; 25(1): 142-146.
  23. Kırmızıbekmez H., Atay İ., Kaiser M., Yeşilada E. Tasdemir D. Antiprotozoal activities of Melampyrum arvense and its secondary metabolites. Planta Medica 2009; 75(09).
  24. Korkotian E., Botalova A., Odegova T., Galishevskaya E., Skryabina E., Segal M. Complex effects of aqueous extract of Melampyrum pratense and of its flavonoids on activity of primary cultured hippocampal neurons. Journal of Ethnopharmacology 2015; 163: 220-228.
  25. Lee YH., Choo C., Watawana MI., Jayawardena N., Waisundara VY. An appraisal of eighteen commonly consumed edible plants as functional food based on their antioxidant and starch hydrolase inhibitory activities. Journal of the Science of Food and Agriculture 2015; 95(14): 2956-2964.
  26. Leventhal LJ., Boyce EG., Zurier RB. Treatment of rheumatoid arthritis with gammalinolenic acid. Annals of Internal Medicine 1993; 119(9): 867-873.
  27. Malek SNA., Shin SK., Wahab A., Yaacob H. Cytotoxic components of Pereskia bleo (Kunth) DC. (Cactaceae) leaves. Molecules 2009; 14(5): 1713-1724.
  28. María Teresa RC., Rosaura VG., Elda CM., Ernesto GP. The avocado defense compound phenol-2,4-bis (1,1-dimethylethyl) is induced by arachidonic acid and acts via the inhibition of hydrogen peroxide production by pathogens. Physiological and Molecular Plant Pathology 2014; 87: 32-41.
  29. Mihoğlugil F., Akalgan D., Tosun F. Cytotoxicity screening of some Turkish plants against renal cancer cells. Journal of Research in Pharmacy 2023; 27(2): 636-641.
  30. Pawar R., Mohandass C., Dastager SG., Kolekar YM., Malwankar R. Antioxidative metabolites synthesized by marine pigmented Vibrio sp. and ıts protection on oxidative deterioration of membrane lipids. Applied Biochemistry and Biotechnology 2016; 179(1): 155-167.
  31. Rangel-Sánchez G., Castro-Mercado E., García-Pineda E. Avocado roots treated with salicylic acid produce phenol-2,4-bis (1,1-dimethylethyl), A compound with antifungal activity. Journal of Plant Physiology 2014; 171(3-4): 189-198.
  32. Re R., Pellegrini N., Proteggente A., Pannala A., Yang M., Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology & Medicine 1999; 26(9-10): 1231-1237.
  33. Slinkard K., Singleton VL. Total phenol analysis: Automation and comparison with manual methods. American Journal of Enology and Viticulture 1977; 28(1): 49-55.
  34. Štajner DI., Popović BM., Boža P., Kapor A. Antioxidant capacity of Melampyrum barbatum weed and medicinal plant. Phytotherapy Research 2009; 23(7): 1006-1010.
  35. Suh HJ., Park S., Park S. Inhibition of browning on fresh apple juices by natural phytochemicals from Rumex crispus L. Seed. Journal of the Korean Society for Applied Biological Chemistry 2011; 54(4): 524-530.
  36. Tank DC., Beardsley PM., Kelchner SA., Olmstead RG. Review of the systematics of Scrophulariaceae s.l. and their current disposition. Australian Systematic Botany 2006; 19(4): 289-307.
  37. Tungmunnithum D., Thongboonyou A., Pholboon A., Yangsabai A. Flavonoids and other phenolic compounds from medicinal plants for pharmaceutical and medical aspects: an overview. Medicines 2018; 5(3): 93.
  38. Varsha KK., Devendra L., Shilpa G., Priya S., Pandey A., Nampoothiri K. M. 2,4-Di-tert-butyl phenol as the antifungal, antioxidant bioactive purified from a newly isolated Lactococcus sp. International Journal of Food Microbiology 2015; 211: 44-50.
  39. Vogl S., Atanasov AG., Binder M., Bulusu M., Zehl M., Fakhrudin N., Heiss EH., Picker P., Wawrosch C., Saukel J., Reznicek G., Urban E., Bochkov V., Dirsch VM., Kopp B. The Herbal Drug Melampyrum pratense L. (Koch): Isolation and identification of ıts bioactive compounds targeting mediators of ınflammation. Evidence-Based Complementary and Alternative Medicine 2013; 26: 395316.
  40. Yu Y., Correll PH., Vanden Heuvel JP. Conjugated linoleic acid decreases production of pro-inflammatory products in macrophages: evidence for a PPARγ-dependent mechanism. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids 2002; 1581(3): 89-99.
  41. Zhang C., Lin Q., ZhangJ., Huang Z., Nan P., Li L., Song Z., Zhang W., Yang J., Wang Y. Comparing complete organelle genomes of holoparasitic Christisonia kwangtungensis (Orabanchaceae) with its close relatives: how different are they? BMC Plant Biology 2022; 22(1): 1-12.
  42. Zhang XH., Zhang EH., Lang DY. Autotoxic compounds from rhizosphere soil of Humulus lupulus L. extracts: ıdentification and biological activity. Agronomy Journal 2011; 103(3): 695-701.
  43. Zhou BL., Chen ZX., Du L., Xie YH., Zhang Q, Ye XL. Allelopathy of root exudates from different resistant eggplants to Verticillium dahliae and the identification of allelochemicals. African Journal of Biotechnology 2011; 10(42): 8284-8290.