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Journals / Mehmet Akif Ersoy Üniversitesi Fen Bilimleri Enstitüsü Dergisi / 2025 / Cilt: 16 Sayı: 2

In Vitro and In Silico Evaluation of Metal Ion Chelated Natural Melanin Nanoparticles on Photothermal Therapy and Magnetic Resonance Performance

Fototermal Tedavi ve Manyetik Rezonans Performansı Üzerine Metal İyonu ile Şelatlanmış Doğal Melanin Nanopartiküllerinin In Vitro ve In Silico Değerlendirilmesi

Melike Demirtaş(BOĞAZİÇİ ÜNİVERSİTESİ)0009-0008-3021-388X
Şeyma Yektar(USKUDAR UNIVERSITY)0009-0003-3814-6806
Gizem Kaleli Can(İZMİR DEMOKRASİ ÜNİVERSİTESİ)0000-0002-4411-622X
İbrahim Alpay Özcan(BOĞAZİÇİ ÜNİVERSİTESİ)0000-0002-3037-2812
Engin Baysoy(BAHÇEŞEHİR ÜNİVERSİTESİ)0000-0001-8323-597X
Mustafa Kemal Ruhi(BOĞAZİÇİ ÜNİVERSİTESİ)0000-0003-3383-7782
Pages
90–99
Publication Date
2025-12-29
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Both computational and experimental results show that Fe³+-chelated MNPs exhibit enhanced PTT performance compared to unchelated MNPs, along with a measurable decrease in MRI contrast following treatment.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Metal ion-chelated natural melanin nanoparticles exhibit strong potential for theranostic applications.
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Key Findings & Data:Melanin nanoparticles (MNPs) and metal ion-chelated MNPs are synthesized, characterized, and evaluated for cytotoxicity in PC-3 cells.
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Conclusion & Impact:Both computational and experimental results show that Fe³+-chelated MNPs exhibit enhanced PTT performance compared to unchelated MNPs, along with a measurable decrease in MRI contrast following treatment.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:doğal melanin nanoparçacıkları · fototermal terapi · kanser terapileri

Abstract

Metal ion-chelated natural melanin nanoparticles exhibit strong potential for theranostic applications. However, cross-effects resulting from modifications, whether introduced externally or occurring spontaneously during application, such as the influence of metal ion chelation on photothermal therapy (PTT) efficiency and the effect of PTT-induced temperature changes on MRI contrast, remain poorly understood. This study investigates these interrelated effects through computational modelling and in vitro experiments. Melanin nanoparticles (MNPs) and metal ion-chelated MNPs are synthesized, characterized, and evaluated for cytotoxicity in PC-3 cells. The PTT performance of the less cytotoxic iron-chelated MNPs is compared to that of the unchelated form using COMSOL simulations and in vitro models. MRI contrast is also assessed before and after PTT. Both computational and experimental results show that Fe³+-chelated MNPs exhibit enhanced PTT performance compared to unchelated MNPs, along with a measurable decrease in MRI contrast following treatment. These findings suggest that Fe3+-chelated MNPs are promising theranostic agents capable of delivering effective PTT while enabling MRI-based dosimetric monitoring during cancer therapy.

Özet

Doğal melanin nanoparçacıklarının (MNP) metal iyonu ile şelatlanmış formları, teranostik uygulamalar için güçlü bir potansiyel sergilemektedir. Bununla birlikte gerek dışarıdan uygulanan modifikasyonların, gerekse uygulama sırasında kendiliğinden ortaya çıkan değişimlerin yol açtığı çapraz etkiler (metal iyon şelatlamasının fototermal terapi (PTT) verimliliği üzerindeki etkisi veya PTT kaynaklı sıcaklık değişimlerinin manyetik rezonans görüntü (MRG) kontrastına etkisi gibi) yeterince anlaşılamamıştır. Bu çalışma, söz konusu karşılıklı etkileri hesaplamalı modelleme ve in vitro deneylerle incelemektedir. Bu çalışmada MNP'ler ve metal iyonu ile şelatlanmış MNP'ler sentezlenmiş, PC-3 hücrelerinde karakterize edilmiş ve sitotoksisiteleri değerlendirilmiştir. Düşük sitotoksisite gösteren demir-şelatlanmış MNP'lerin PTT performansı, şelatlanmamış form ile COMSOL simülasyonları ve in vitro modeller kullanılarak karşılaştırılmıştır. Ayrıca PTT öncesi ve sonrasında MRG kontrastı değerlendirilmiştir. Hesaplamalı ve deneysel bulguların her ikisi de Fe3+-şelatlanmış MNP'lerin şelatlanmamış MNP'lere kıyasla daha yüksek PTT performansı sergilediğini ve tedavi sonrasında MRG kontrastında ölçülebilir bir azalma meydana geldiğini göstermiştir. Bu bulgular, Fe3+-şelatlanmış MNP'lerin etkili bir PTT sağlarken aynı zamanda kanser tedavisi sırasında MRG tabanlı dozimetrik izleme olanağı sunan umut verici teranostik ajanlar olduğunu düşündürmektedir.

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Cite This Article

Demirtaş, M., Yektar, Ş., Can, G. K., Özcan, İ. A., Baysoy, E., Ruhi, M. K. (2025). In Vitro and In Silico Evaluation of Metal Ion Chelated Natural Melanin Nanoparticles on Photothermal Therapy and Magnetic Resonance Performance. Mehmet Akif Ersoy Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Cilt: 16 Sayı: 2, 90–99. https://doi.org/10.29048/makufebed.1798224

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