Journals / Kommagene Biyoloji Dergisi / 2021 / Cilt: 5 Sayı: 2

Düzeltme: Molecular Characterization and Determination of Some Biochemical Properties of Endemic Serratula olygocephala Growing in Adıyaman

Düzeltme: Düzeltme: Adıyaman'da Yetişen Endemik Serratula olygocephala’nın Moleküler Karakterizasyonu ve Bazı Biyokimyasal Özelliklerini Belirlenmesi

Yener Tekeli(ADIYAMAN ÜNİVERSİTESİ, ECZACILIK FAKÜLTESİ)0000-0003-1524-457X
Ahmet Özkaya(ADIYAMAN ÜNİVERSİTESİ)
Ahmet Zafer Tel(ADIYAMAN ÜNİVERSİTESİ)
Görkem Deniz Sönmez(ADIYAMAN ÜNİVERSİTESİ)
Süleyman Akocak(ADIYAMAN ÜNİVERSİTESİ)
Pages
222–222
Publication Date
2021-12-31
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The percentage of the saturated, monounsaturated, and polyunsaturated fatty acids was calculated.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, molecular characterization of endemic Serratula olygocephala grown in Adiyaman was determined.
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Key Findings & Data:DNA isolation was performed using the Plant DNeasy (Qiagen Inc.) kit, following the kit protocol.
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Conclusion & Impact:The percentage of the saturated, monounsaturated, and polyunsaturated fatty acids was calculated.
🔬Design:Düzeltme
📍Scope:Comprehensive Sample
⚙️Variables:Serratula oligocephala · moleküler karakterizasyon · antioksidan

Abstract

In this study, molecular characterization of endemic Serratula olygocephala grown in Adiyaman was determined. DNA isolation was performed using the Plant DNeasy (Qiagen Inc.) kit, following the kit protocol. In addition, the antioxidant activity and fatty acid composition of the plant were also determined. In terms of the antioxidant activity, total phenolic substance concentration of the plant was determined by applying the folin method and free radical scavenging capacity was determined by DPPH radical scavenging test. Fatty acid profile of Serratula oligocephala was determined by gas chromatography. The percentage of the saturated, monounsaturated, and polyunsaturated fatty acids was calculated.

Özet

Bu çalışmada Adıyaman'da Yetişen Endemik Serratula olygocephala’nın moleküler karakterizasyonu yapıldı. DNA izolasyonu Plant DNeasy (Qiagen Inc.) kiti kullanılarak, kit protokolü takip edilerek yapıldı. Bunun yanında bitkinin antioksidan aktivitesi ve yağ asidi kompozisyonu da belirlenmiştir. Antioksidan etkinliği bakımından, bitkinin toplam fenolik madde konsantrasyonu folin yöntemiyle belirlendi ve serbest radikal süpürme kapasitesini ise invitro olarak DPPH radikal süpürme testi ile tayin edildi. Serratula olygocephala’nın yağ asidi kompozisyonu ise gaz kromatografi yöntemiyle tayin edildi. Doymuş, tekli doymamış ve çoklu doymamış yağ asitleri yüzdesi belirlenmiştir.

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Cite This Article

Tekeli, Y., Özkaya, A., Tel, A. Z., Sönmez, G. D., Akocak, S. (2021). Düzeltme: Molecular Characterization and Determination of Some Biochemical Properties of Endemic Serratula olygocephala Growing in Adıyaman. Kommagene Biyoloji Dergisi, Cilt: 5 Sayı: 2, 222–222. https://doi.org/10.31594/commagene.1033243

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References (4)

  1. Sang, T., Crawford, D.J., & Stuessy, T.F. (1995). Documentation of reticulate evolution in peonies (Paeonia) using internal transcribed spacer sequences of nuclear ribosomal DNA: Implications for biogeography and concerted evolution. Proceedings of the National Academy of Sciences of the United States of America, 92, 6813-6817. https://doi.org/10.1073/pnas.92.15.6813
  2. Taberlet, P., Gielly, L., Pautou, G., & Bouvet, J. (1991). Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Molecular Biology, 17, 1105–1109.
  3. Tekeli, Y., Özkaya, A., Tel, A.Z., Deniz Sönmez, G., & Akocak, S. (2019). Molecular Characterization and Determination of Some Biochemical Properties of Endemic Serratula olygocephala Growing in Adıyaman. Commagene Journal of Biology, 3 (1), 32-36. https://doi.org/10.31594/commagene.789682
  4. White T. J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, In: M Innis, D Gelfald, J Sninsky, T White, (eds.) PCR Protocols: A Guide to Methods and Applications. pp. 315-322. San Francisco: Academic Press. https://doi.org/10.1590/0102-33062015abb0267