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In Vitro Effects of Hypericum pseudolaeve N. Robson Aqueous Extract on the Expression of Wound Healing-Related Genes in L929 Fibroblast Cells

Hypericum pseudolaeve N. Robson Sulu Ekstresinin L929 Fibroblast Hücrelerinde Yara İyileşmesi ile İlgili Genlerin Ekspresyonu Üzerine In Vitro Etkileri

Bahar Kaptaner İğci(ANKARA ÜNİVERSİTESİ)0000-0001-7102-6337
Zeki Aytaç(GAZİ ÜNİVERSİTESİ)0000-0003-3244-3183
Pages
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Publication Date
2026-10-22
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Overall, the results indicate that Hypericum pseudolaeve aqueous extract can promote the expression of key mediators involved in extracellular matrix formation, growth factor signaling, and angiogenesis, which may play role its wound-healing potential.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Wound healing is a complex process involving extracellular matrix remodeling, angiogenesis, and tissue regeneration.
📊
Key Findings & Data:This study investigated the effects of the water-macerated extract of Hypericum pseudolaeve N.
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Conclusion & Impact:Overall, the results indicate that Hypericum pseudolaeve aqueous extract can promote the expression of key mediators involved in extracellular matrix formation, growth factor signaling, and angiogenesis, which may play role its wound-healing potential.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Hypericum pseudolaeve · yara iyileşmesi · L929 fibroblastları

Abstract

Wound healing is a complex process involving extracellular matrix remodeling, angiogenesis, and tissue regeneration. Although medicinal plants are widely recognized for their therapeutic potential, the molecular basis of their wound-healing activity remains incompletely understood. This study investigated the effects of the water-macerated extract of Hypericum pseudolaeve N. Robson (HWM) on the expression of wound healing–related genes in L929 fibroblast cells. Cells were treated with HWM extract (62 µg/mL) for 24 h and the expression of collagen, FGF7, TGF-β1, and VEGF was evaluated using qRT-PCR and ELISA methods. HWM treatment increased the expression of collagen, FGF7, TGF-β1, and VEGF by 2.0, 3.8, 47.9, and 3.6-fold, respectively, compared with untreated controls. ELISA analyses supported the qRT-PCR findings by demonstrating elevated secreted protein levels of the investigated markers. Among the analyzed genes, TGF-β1 exhibited the most pronounced statistically significant response in both methods. Overall, the results indicate that Hypericum pseudolaeve aqueous extract can promote the expression of key mediators involved in extracellular matrix formation, growth factor signaling, and angiogenesis, which may play role its wound-healing potential.

Özet

Yara iyileşmesi; ekstraselüler matriksin yeniden düzenlenmesi, anjiyogenez ve doku rejenerasyonunu kapsayan karmaşık bir biyolojik süreçtir. Tıbbi bitkiler terapötik potansiyelleri nedeniyle yaygın olarak kullanılmaktadır. Ancak bu bitkilerin yara iyileşmesi üzerindeki etkilerinin moleküler mekanizmaları henüz tam olarak açıklanamamıştır. Bu çalışmada, Hypericum pseudolaeve N. Robson'un su maserasyonu yöntemiyle hazırlanan ekstraktının (HWM), L929 fibroblast hücrelerinde yara iyileşmesiyle ilişkili genlerin ifadesi üzerindeki etkileri araştırılmıştır. L929 fibroblast hücreleri, 62 µg/mL konsantrasyondaki HWM ekstraktı ile 24 saat boyunca inkübe edilmiştir. Kolajen, fibroblast büyüme faktörü 7 (FGF7), dönüştürücü büyüme faktörü-β1 (TGF-β1) ve vasküler endotelyal büyüme faktörü (VEGF) genlerinin ekspresyon düzeyleri qRT-PCR ve ELISA yöntemleri kullanılarak belirlenmiştir. HWM uygulaması sonucunda kolajen, FGF7, TGF-β1 ve VEGF genlerinin ifade düzeyleri kontrol grubuna göre sırasıyla 2,0; 3,8; 47,9 ve 3,6 kat artmıştır. ELISA analizleri, incelenen belirteçlerin protein düzeylerindeki artışı ortaya koymuş ve qRT-PCR sonuçlarını desteklemiştir. İncelenen genler arasında en yüksek artış TGF-β1 ifadesinde belirlenmiştir. Elde edilen bulgular, HWM ekstraktının ekstraselüler matriks oluşumu, büyüme faktörü sinyal iletimi ve anjiyogenez süreçlerinde görev alan temel aracı moleküllerin ekspresyonunu artırdığını göstermektedir. Sonuç olarak, Hypericum pseudolaeve'nin yara iyileşmesini destekleyebilecek önemli bir biyolojik potansiyele sahip olduğu değerlendirilmiştir.

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Cite This Article

İğci, B. K., Aytaç, Z. (2026). In Vitro Effects of Hypericum pseudolaeve N. Robson Aqueous Extract on the Expression of Wound Healing-Related Genes in L929 Fibroblast Cells. Kommagene Biyoloji Dergisi, Cilt: 10 Sayı: 1. https://doi.org/10.31594/commagene.1965622

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