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Journals / Kommagene Biyoloji Dergisi / 2026 / Cilt: 7 Sayı: 1

Effects of 3-Benzoyl-7-Hydroxy Coumarin Compound on TNF-α and Caspase-3 Immunreactivity in Kidneys of Rats Exposed to Aluminum

Alüminyuma Maruz Kalan Sıçanların Böbreklerinde 3-Benzoil-7-Hidroksi Kumarin Bileşiğinin TNF-α ve Kaspaz-3 İmmün Reaktivitesi Üzerine Etkileri

Ahmet Türk(ADIYAMAN UNIVERSITY)0000-0003-0903-3522
Tuncay Kuloğlu(FIRAT ÜNİVERSİTESİ)0000-0001-9874-3838
Büşra Zencirci(Adıyaman Üniversitesi)0000-0002-6015-3047
Ali Gürel(FIRAT ÜNİVERSİTESİ)0000-0001-8087-8814
Abdullah Karadağ(ADIYAMAN ÜNİVERSİTESİ)0000-0003-3620-9589
Yusuf Özay(ADIYAMAN ÜNİVERSİTESİ)0000-0003-3855-6197
Serhat Hançer(FIRAT ÜNİVERSİTESİ)0000-0003-3237-3672
Muhammed Emre Yüzer(ADIYAMAN ÜNİVERSİTESİ)0000-0002-0115-9473
Kenan Türkan(ADIYAMAN ÜNİVERSİTESİ)0000-0002-7379-8995
Ahmet Özkaya(ADIYAMAN ÜNİVERSİTESİ)0000-0002-0173-3084
Pages
73–78
Publication Date
2023-06-30
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

In according to our data, Alu administration caused increased Caspase-3 TNF-α and MDA levels in kidney tissue, and 3-benzoyl-7-hydroxy coumarin was found to significantly reverse these changes.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, we aimed to investigate the effects of 3-benzoyl-7-hydroxy coumarin compound on apoptotic markers in the kidney tissues of rats treated with Aluminum(Alu). 28 Wistar albino male rats were divided into four groups (n = 7) as: Control Group: 1 ml/kg 0.9% NaCl was administered by oral gavage every other day during the experiment.
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Key Findings & Data:Aluminium (Alu) Group: Alu was prepared in water and given by intraperitoneal(i.p) for 30 days at an excess of 8.3 mg/kg/day.
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Conclusion & Impact:In according to our data, Alu administration caused increased Caspase-3 TNF-α and MDA levels in kidney tissue, and 3-benzoyl-7-hydroxy coumarin was found to significantly reverse these changes.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Oksidatif stres · Apoptoz · Proinflamatuar sitokin

Abstract

In this study, we aimed to investigate the effects of 3-benzoyl-7-hydroxy coumarin compound on apoptotic markers in the kidney tissues of rats treated with Aluminum(Alu). 28 Wistar albino male rats were divided into four groups (n = 7) as: Control Group: 1 ml/kg 0.9% NaCl was administered by oral gavage every other day during the experiment. Aluminium (Alu) Group: Alu was prepared in water and given by intraperitoneal(i.p) for 30 days at an excess of 8.3 mg/kg/day. Treatment Group: 10 mg/kg 3-Benzoyl-7-Hydroxy Coumarin was administered to rats by oral gavage every other day for 30 days. Alu + Treatment Group: 8.3 mg/kg Alu was prepared in water and administered i.p for 30 days every other day, and then 10 mg/kg 3-Benzoyl-7-Hydroxy Coumarin was administered to rats by oral gavage every other day for 30 days. At the end of the 30-day experiment, the kidney tissue of the animals were removed under anesthesia and stored under appropriate conditions. In according to our data, Alu administration caused increased Caspase-3 TNF-α and MDA levels in kidney tissue, and 3-benzoyl-7-hydroxy coumarin was found to significantly reverse these changes.

Özet

Bu çalışmada, Alüminyum (Alu) ile tedavi edilen sıçanların böbrek dokularında 3-benzoil-7-hidroksi kumarin bileşiğinin apoptotik belirteçler üzerindeki etkilerini araştırmayı amaçladık. Deney için 28 adet Wistar albino erkek ratlar 4 gruba (n=7) ayrıldı; Kontrol Grubu: Deney süresince sıçanlara gün aşırı 1 ml/kg %0.9 NaCl oral gavaj yolu ile verildi. Alüminyum (Alu) Grubu: Suda çözünmüş, Alu bileşiği intraperitonal(i.p.) yol ile 30 gün boyunca 8.3 mg/kg/gün aşırı uygulandı. Tedavi Grubu: Sıçanlara gün aşırı 10 mg/kg 3-Benzoyl-7-Hydroxy kumarin oral gavaj yolu ile 30 gün süreyle uygulandı. Alu + Tedavi Grubu: 8.3 mg/kg Alu su içinde hazırlandı ve i.p. deney süresi boyunca gün aşırı 10 mg/kg 3-Benzoyl-7-Hydroxy kumarin oral gavaj ile uygulandı. 30 günlük deney sonunda anestezi altında hayvanların böbrek dokusu alınarak uygun koşullarda saklandı. Elde edilen verilere göre Alu uygulaması böbrek dokusunda Kaspaz-3, TNF-α ve MDA düzeylerinin artmasına neden olmuş ve 3-benzoil-7-hidroksi kumarin bileşiğinin bu değişiklikleri önemli ölçüde tersine çevirdiği görüldü.

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Cite This Article

Türk, A., Kuloğlu, T., Zencirci, B., Gürel, A., Karadağ, A., Özay, Y., Hançer, S., Yüzer, M. E., Türkan, K., Özkaya, A. (2023). Effects of 3-Benzoyl-7-Hydroxy Coumarin Compound on TNF-α and Caspase-3 Immunreactivity in Kidneys of Rats Exposed to Aluminum. Kommagene Biyoloji Dergisi, Cilt: 7 Sayı: 1, 73–78. https://doi.org/10.31594/commagene.1217426

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