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Journals / Kommagene Biyoloji Dergisi / 2026 / Cilt: 10 Sayı: 1

The First Record of Dina sivricensis (Hirudinea: Erpobdellidae) from Duhok Province, Iraq, Based on COI Barcoding

COI Barkodlama Temelinde Dina sivricensis (Hirudinea: Erpobdellidae)'in Irak'ın Duhok İlinden İlk Kaydı

Hamid Kachel(Zakho)0000-0002-7231-8267
Imad Omer(Zakho)0009-0006-8114-6635
Barwar Sadeeq(Zakho)0009-0009-7279-8182
Farhad Ali(zakho)0000-0002-8645-6564
Naim Saglam(FIRAT UNIVERSITY)0000-0002-3163-8432
Pages
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Publication Date
2026-07-14
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This study has recorded D. sivricensis from Iraq, thereby extending its known geographic range and contributing to the recorded diversity of erpobdellids in Iraq.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Erpobdellid leeches are important freshwater predators that frequently pose major taxonomic problems due to their high morphological similarity.
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Key Findings & Data:Dina Blanchard, 1892, is a genus distributed exclusively throughout Eurasia and has been differentiated from related genera by its mid-body annulation.
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Conclusion & Impact:This study has recorded D. sivricensis from Iraq, thereby extending its known geographic range and contributing to the recorded diversity of erpobdellids in Iraq.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Sülük · moleküler tanımlama · filogenetik analiz

Abstract

Erpobdellid leeches are important freshwater predators that frequently pose major taxonomic problems due to their high morphological similarity. Dina Blanchard, 1892, is a genus distributed exclusively throughout Eurasia and has been differentiated from related genera by its mid-body annulation. This study presents the first molecular evidence for Dina sivricensis from Duhok Province, Iraq. Recently recorded as a new species from Türkiye, D. sivricensis is distinguished by its small cylindrical body and unique reproductive organs, including a small spherical atrium and an ellipsoidal atrial cornea. Species identification was verified using cytochrome c oxidase subunit I (COI) barcoding. The sequence obtained in this study (PZ144795) showed 95%–97% similarity to reference sequences of D. sivricensis, which were deposited under accession numbers MH013409 and MG949122 in GenBank. This study has recorded D. sivricensis from Iraq, thereby extending its known geographic range and contributing to the recorded diversity of erpobdellids in Iraq.

Özet

Erpobdellid sülükleri, yüksek morfolojik benzerlikleri nedeniyle sıklıkla büyük taksonomik problemlere yol açan önemli tatlı su yırtıcılarıdır. Sadece Avrasya genelinde yayılış gösteren Dina Blanchard 1892 cinsi, vücut ortasındaki halka yapısı (anülasyon) ile akraba cinslerden ayırt edilmiştir. Bu çalışma, Irak'ın Duhok Valiliği'nden Dina sivricensis türüne ait ilk moleküler kanıtları sunmaktadır. Yakın zamanda Türkiye'den yeni bir tür olarak kaydedilen D. Sivricensis cinsinden küçük silindirik vücudu, küçük küresel atrium ve elipsoidal atrial korneayı içeren benzersiz üreme organları ile ayırt edilir. Tür teşhisi, sitokrom c oksidaz alt birim I (COI) barkodlaması kullanılarak doğrulanmıştır. Bu çalışmada elde edilen dizi (PZ144795), GenBank'ta MH013409 ve MG949122 erişim numaralarıyla kayıtlı D. sivricensis referans dizileri ile %95-%97 oranında benzerlik göstermiştir. Bu çalışma D. sivricensis'i Irak'tan kaydederek türün bilinen coğrafî dağılımını genişletmiş ve Irak'taki erpobdellid çeşitliliğine katkıda bulunmuştur.

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Cite This Article

Kachel, H., Omer, I., Sadeeq, B., Ali, F., Saglam, N. (2026). The First Record of Dina sivricensis (Hirudinea: Erpobdellidae) from Duhok Province, Iraq, Based on COI Barcoding. Kommagene Biyoloji Dergisi, Cilt: 10 Sayı: 1. https://doi.org/10.31594/commagene.1964163

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References (17)

  1. Ali, L.A., & Jawair, H.J. (2013). First Record of Dina Lineata (Hirudinea: Erpobdellidae) in Greater Zab River Kurdistan Region-Iraq. Science Journal of University of Zakho, 1(1), 207–209.
  2. Bilal, S., Ali, L., Abdullah, L., Khailany, R., Dhahir, S., & Abdullah, S. (2017). First record of leech Dina punctata (Annelida: Erpobdellidae) from Lesser Zab River in northern Iraq: Morphological and molecular investigation. Jordan Journal of Biological Science, 10(2), 69–72.
  3. Folmer, O., Black, M., Hoeh, W., Lutz, R., & Vrijenhoek, R. (1994). DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3(5), 294–299.
  4. Kimura, M. (1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16(2), 111–120. https://doi.org/10.1007/bf01731581
  5. Kutschera, U. (2003). The feeding strategies of the leech Erpobdella octoculata (L.): A laboratory study. International Review of Hydrobiology, 88(1), 94–101.
  6. Nei, M., & Kumar, S. (2000). Molecular evolution and phylogenetics. Oxford university press.
  7. Nesemann, H., & Neubert, E. (1994). New data to the leeches of the subfamily Trochetinae (Hirudinea, Erpobdellidae). Miscellanea Zoologica Hungarica, 9, 19–28.
  8. Nesemann, H., & Neubert, E. (1999). Annelida, Clitellata: Branchiobdellida, Acanthobdellea, Hirudinea. In J. Schwoerbel & P. Zwick (Eds.), Süßwasserfauna von Mitteleuropa (Vol. 6/2, pp. 1–178). Spektrum Akademischer Verlag.
  9. Pešić, V., & Grosser, C. (2022). Dina serbica, a new species of leeches (Annelida: Hirudinea: Erpobdellidae) from Serbia, based on morphological and molecular evidence. Ecologica Montenegrina, 51, 1–14.
  10. Saglam, N., Kutschera, U., Saunders, R., Saidel, W.M., Balombini, K.L.W., & Shain, D.H. (2018). Phylogenetic and morphological resolution of the Helobdella stagnalis species-complex (Annelida: Clitellata: Hirudinea). Zootaxa, 4403(1), 61–86. https://doi.org/10.11646/zootaxa.4403.1.3
  11. Saglam, N., Saunders, R., & Shain, D.H. (2025). Species delimitation recovers undescribed Erpobdellidae (Annelida: Hirudinea) from USA and Türkiye. Zootaxa, 5627(3), 455–479. https://doi.org/10.11646/zootaxa.5627.3.3
  12. Siddall, M.E. (2002). Phylogeny of the leech family Erpobdellidae (Hirudinida: Oligochaeta). Invertebrate Systematics, 16, 1–6.
  13. Sket, B., & Trontelj, P. (2008). Global diversity of leeches (Hirudinea) in freshwater. Hydrobiologia, 595, 129–137. https://doi.org/10.1007/s10750-007-9010-8
  14. Stucky, B.J. (2012). SeqTrace: a graphical tool for rapidly processing DNA sequencing chromatograms. Journal of biomolecular techniques, 23(3), 90–93. https://doi.org/10.7171/jbt.12-2303-004
  15. Tamura, K. (1992). Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G+C-content biases. Molecular Biology and Evolution, 9(4), 678–687. https://doi.org/10.1093/oxfordjournals.molbev.a040752
  16. Tamura, K., Stecher, G., & Kumar, S. (2021). MEGA11: Molecular Evolutionary Genetics Analysis Version 11. Molecular Biology and Evolution, 38(7), 3022–3027. https://doi.org/10.1093/molbev/msab120
  17. Zewayee, F.A., & Ali, L.A. (2024). The first record of Dina prokletijaca (Hirudinida: Erpobdellidae) from Bekhal stream, Erbil, Iraq. Journal of Wildlife and Biodiversity, 8(1), 54–64.